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AB300724

Anti-Calcineurin B/PPP3R1+Calcineurin BII/PPP3R2 antibody [EPR24992-14] -BSA & Azide free (Detector)

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Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1, CBLP, PPP3RL, PPP3R2, Calcineurin subunit B type 2, Calcineurin B-like protein, Calcineurin BII, PPP3R1-like, Protein phosphatase 2B regulatory subunit 2, Protein phosphatase 3 regulatory subunit B beta isoform, CNBII

2 Images
Sandwich ELISA - Anti-Calcineurin B/PPP3R1+Calcineurin BII/PPP3R2 antibody [EPR24992-14] -BSA & Azide free (Detector) (AB300724)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Calcineurin B/PPP3R1+Calcineurin BII/PPP3R2 antibody [EPR24992-14] -BSA & Azide free (Detector) (AB300724)

Sandwich ELISA of ab300722 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

ELISA - Anti-Calcineurin B/PPP3R1+Calcineurin BII/PPP3R2 antibody [EPR24992-14] -BSA & Azide free (Detector) (AB300724)
  • ELISA

Lab

ELISA - Anti-Calcineurin B/PPP3R1+Calcineurin BII/PPP3R2 antibody [EPR24992-14] -BSA & Azide free (Detector) (AB300724)

Direct ELISA showed that ab300724 binds to human Calcineurin B/PPP3R1 and Calcineurin BII/PPP3R2.

  • Carrier free

    Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free

  • Unconjugated

    Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24992-14

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB282104

Anti-Calcineurin A antibody [EPR24997-22]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

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Primary Antibodies

AB45689

Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal (AB45689)

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Primary Antibodies

AB2796

Anti-NFAT2 antibody [7A6]

BOND RX™ Validated|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-NFAT2 antibody [7A6] (AB2796)

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Primary Antibodies

AB24373

Anti-FKBP12 antibody

KO Validated

Western blot - Anti-FKBP12 antibody (AB24373)

  • 5
  • 4
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Reactivity data

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Product details

ab300724 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human Calcineurin B/CNB. The recommended pair for sandwich ELISA is:
Capture: ab200723, human Calcineurin B/CNB Capture Antibody (unconjugated)
Detector: ab300724, human Calcineurin B/CNB Detector Antibody (unconjugated)
The reference range value is 0.16-20 ng/mL.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcineurin B is a regulatory subunit of the enzyme calcineurin known alternatively as PPP3R1 for Calcineurin BI and PPP3R2 for Calcineurin BII. This protein weighs approximately 19 kDa. Calcineurin B is expressed in various tissues including brain muscle and immune cells. Its primary function is to bind calcium ions facilitating conformational changes that enable the activation of the catalytic subunit of calcineurin. Through these changes it maintains a connection between calcium signaling and other cellular processes.
Biological function summary

Calcineurin B plays an essential role in intracellular signaling by being part of the calcineurin complex which also includes the catalytic subunit calcineurin A. This complex is vital for the dephosphorylation of the nuclear factor of activated T-cells (NFAT) which then translocates to the nucleus to influence the expression of various genes. By controlling gene transcription calcineurin B contributes significantly to immune response muscle development and brain function.

Pathways

Calcineurin B facilitates various cellular reactions as an integral component of calcium signaling and immune response. It is significantly involved in the T-cell receptor signaling pathway where its activity helps regulate T-cell activation and differentiation. Calcineurin B also plays a role in the synaptic plasticity pathway important for learning and memory functions in the brain. It interacts closely with proteins like NFATc and calmodulin that further extend its influence across other signaling pathways.

Calcineurin B is implicated in conditions such as autoimmune diseases and cardiac hypertrophy. In autoimmune diseases disrupted calcineurin function can lead to improper T-cell activity contributing to diseases like rheumatoid arthritis. In cardiac hypertrophy altered calcineurin signaling can affect heart muscle growth and function. Calcineurin B is connected to the protein FKBP12 which is part of the immunosuppressive drug complex influencing its role in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
See full target information PPP3R1

Additional targets

PPP3R2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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