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AB248891

Anti-Calcipressin 1/RCAN1 antibody [EPR8911] - BSA and Azide free

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Rabbit Recombinant Monoclonal Calcipressin 1/RCAN1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

ADAPT78, CSP1, DSC1, DSCR1, RCAN1, Calcipressin-1, Adapt78, Down syndrome critical region protein 1, Myocyte-enriched calcineurin-interacting protein 1, Regulator of calcineurin 1, MCIP1

1 Images
Western blot - Anti-Calcipressin 1/RCAN1 antibody [EPR8911] - BSA and Azide free (AB248891)
  • WB

Unknown

Western blot - Anti-Calcipressin 1/RCAN1 antibody [EPR8911] - BSA and Azide free (AB248891)

This data was developed using ab140131, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calcipressin 1/RCAN1 antibody [EPR8911] (<a href='/en-us/products/primary-antibodies/calcipressin-1-rcan1-antibody-epr8911-ab140131'>ab140131</a>) at 1/10000 dilution

All lanes:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8911

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248891 is the carrier-free version of ab140131.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcipressin 1 also known as RCAN1 is a protein that plays a central role in regulating calcineurin activity. This protein weighs around 29-31 kDa. It is found in various human tissues especially in the heart brain and skeletal muscle. By binding to calcineurin RCAN1 inhibits its phosphatase activity influencing cellular pathways that rely on calcium signaling.
Biological function summary

Within the cell RCAN1 functions as a modulator of calcineurin-dependent transcriptional activity. It interacts with several proteins including those in the signaling complex linked to the NFAT (nuclear factor of activated T-cells). By managing the activity of calcineurin RCAN1 influences the transcription of genes involved in growth development and immune responses. Therefore its role is critical in maintaining cellular homeostasis and response to changes in calcium levels.

Pathways

RCAN1 impacts the calcineurin-NFAT signaling and MAPK (mitogen-activated protein kinase) pathways. In the calcineurin-NFAT pathway RCAN1 influences T-cell activation and the immune response by modulating NFAT's transcriptional activity. Moreover the MAPK pathway which is important for cell proliferation and differentiation sees regulation through RCAN1's action on calcineurin interfacing with kinases like JNK (c-Jun N-terminal kinase) to adjust signaling outputs.

Alterations in RCAN1 expression or function associate with Down syndrome and Alzheimer's disease. Increased RCAN1 levels often seen in Down syndrome lead to reduced calcineurin function affecting neuronal signaling pathways. In Alzheimer's disease the interaction with proteins like Tau contributes to neurodegenerative processes. Understanding RCAN1's involvement provides insights into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A (PubMed : 12809556). Could play a role during central nervous system development (By similarity).
See full target information RCAN1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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