Anti-Calcitonin antibody [16B5]

• Flow Cyt

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Flow Cytometry - Anti-Calcitonin antibody [16B5] (AB11493)

Overlay histogram showing SH-SY5Y cells stained with ab11493 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11493, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366<, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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• WB

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Western blot - Anti-Calcitonin antibody [16B5] (AB11493)

Immunodetection of human recominbinant procalcitonin in WB by a range of monoclonal antibodies available which detect different fragments of the protein, N-terminal, calcitonin or catacalcin, (see immunogen section and related figure).

Anti- N-terminal antibodies available; 1) ab14816 [6F10], 2) ab14813 [27A3], 3) ab11498 [38F11], 4) ab14817 [44D9], 5) ab11494 [42],

Anti- calcitonin antibodies available; 6) ab11484 [13B9], 7) ab14818 [13F2], 8) ab14819 [13G11], 9) ab11496 [14A2], 10) ab11493 [16B5], 11) ab11497 [24B2],

Anti- catacalcin antibodies available; 12) ab11487 [14C12], 13) ab11490 [18B7], 14) ab11491 [22A11].

Immunodetection of human recominbinant procalcitonin in WB by a range of monoclonal antibodies available which detect different fragments of the protein, N-terminal, calcitonin or catacalcin, (see immunogen section and related figure).

Anti- N-terminal antibodies available; 1) ab14816 [6F10], 2) ab14813 [27A3], 3) ab1149

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Calcitonin antibody [16B5] (ab11493)

Predicted band size: 15 kDa

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• sELISA

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Sandwich ELISA - Anti-Calcitonin antibody [16B5] (AB11493)

Calibration curves of several procalcitonin sandwich ELISAs using a range of monclonal antibodies available agaisn't procalcitonin, calcitonin and catacalcin fragments. Capture antibodies used at 1 μg/well and detection antibodies at 0.1 μg/well.

Dark blue square = ab11493 [16B5] (calcitonin fragment) and ab11494 [42] (procalcitonin fragment). Light grey circle = ab11497 [24B2] (calcitonin fragment) and ab11484 [13B9] (calcitonin fragment). Grey triangle = ab11487 [14C12] (catacalcin fragment) and ab11496 [14A2] (calcitonin fragment). Dark grey circle = ab14813 [27A3] (procalcitonin fragment) and ab11491 [22A11] (catacalcin fragment).

This image was generated using the ascites version of the product.

• sELISA

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Sandwich ELISA - Anti-Calcitonin antibody [16B5] (AB11493)

Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml

This image was generated using the ascites version of the product.

• Schematic Diagram

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Schematic Diagram - Anti-Calcitonin antibody [16B5] (AB11493)

Amino acid sequence and schematic diagram of human procalcitonin and the N-terminal, calcitonin, and katacalcin (catacalcin) fragments.

This image was generated using the ascites version of the product.