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AB133235

Anti-Calcitonin antibody [EPR68(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal Calcitonin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CALC1, CALCA, Calcitonin

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)

Immunohistochemical staining of Calcitonin in paraffin embedded Human medullary carcinoma of thyroid gland using ab133235 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133235 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)

Immunohistochemical analysis of paraffin embedded Human Prostatic carcinoma tissue using ab133235 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)

Immunohistochemical analysis of paraffin embedded Human Papillary carcinoma of thyroid gland tissue using ab133235 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)

Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab133235 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [EPR68(2)] (AB133235)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133235 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Carrier free

    Anti-Calcitonin antibody [EPR68(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR68(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcitonin also known as thyrocalcitonin is a peptide hormone with a molecular mass of about 3.4 kDa. It consists of 32 amino acids and is primarily synthesized in parafollicular cells or C cells of the thyroid gland. This hormone regulates calcium and phosphate homeostasis in the body. It reduces blood calcium levels by inhibiting osteoclast activity in the bones and decreasing reabsorption in the kidneys leading to more calcium excretion. Calcitonin also interacts with its receptor a G protein-coupled receptor found in tissues like bone kidney and brain.
Biological function summary

This hormone plays a significant role in maintaining calcium levels by balancing the actions of parathyroid hormone (PTH). Calcitonin works independently but interacts with the broader system managing bone remodeling through an intricate network. The presence of calcitonin in specialized receptor complexes allows it to exert its effects efficiently in target tissues making it an important player in calcium and phosphorus metabolism.

Pathways

Calcitonin acts within the calcium signaling pathway and the bone resorption pathway. Through these pathways calcitonin works alongside other proteins such as parathyroid hormone (PTH) and calcitonin gene-related peptide (CGRP) maintaining skeletal integrity. It modulates the activity of osteoclasts ensuring that bone resorption activities are counterbalanced by bone formation and influences intracellular calcium concentration by binding to its receptor on target cells.

Calcitonin is linked to conditions such as osteoporosis and medullary thyroid carcinoma. Osteoporosis characterized by decreased bone density and increased fracture risk involves dysregulated calcium and phosphate balance. Proteins like estrogen and growth factors along with calcitonin influence bone health. Medullary thyroid carcinoma a type of thyroid cancer arising from C cells affects calcitonin levels with this hormone serving as a biomarker for diagnosing and monitoring treatment effectiveness. Calcitonin's connection with these diseases highlights its diagnostic and therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed : 35324283).. Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.
See full target information CALCA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:5422 PubMed35361811

2022

A clinically relevant heterozygous ATR mutation sensitizes colorectal cancer cells to replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Egger,Benoît Bordignon,Arnaud Coquelle
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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