AB16697

Anti-Calcitonin antibody [SP17]

Name: Anti-Calcitonin antibody [SP17] (ab16697) | Abcam
Brand: Abcam Limited
SKU: AB16697
Price: 895 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Anti-Calcitonin antibody [SP17] (ab16697) is a rabbit monoclonal antibody detecting Calcitonin in IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2005

View Alternative Names

CALC1, CALCA, Calcitonin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (AB16697)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (AB16697)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

This image was generated using the hybridoma version of the product.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (AB16697)

Formalin-fixed, paraffin-embedded human thyroid medullary carcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

This image was generated using the hybridoma version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin antibody [SP17] (AB16697)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin antibody [SP17] (AB16697)

Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Calcitonin with purified ab16697 at 1/50 (2.4 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated using the hybridoma version of the product.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (AB16697)

Formalin-fixed, paraffin-embedded human pituitary tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

This image was generated using the hybridoma version of the product.

Carrier free

Anti-Calcitonin antibody [SP17] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Calcitonin antibody [SP17]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Calcitonin antibody [SP17]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Calcitonin antibody [SP17]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Calcitonin antibody [SP17]
578 PE

PE Anti-Calcitonin antibody [SP17]
660 APC

APC Anti-Calcitonin antibody [SP17]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Calcitonin antibody [SP17]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP17

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Calcitonin antibody [SP17] (ab16697) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Collaboration
Anti-Calcitonin [SP17] (ab16697) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products
We have a range of other formats of antibody clone [SP17] also available for your convenience: ab16697, ab238807, Carrier free - ab240922, Alexa Fluor® 488 - ab320486, Alexa Fluor® 647 - ab320487, Alexa Fluor® 555 - ab320488, Alexa Fluor® 594 - ab320489, PE - ab320490, APC - ab320491, Alexa Fluor® 750 - ab321154

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcitonin also known as thyrocalcitonin is a peptide hormone with a molecular mass of about 3.4 kDa. It consists of 32 amino acids and is primarily synthesized in parafollicular cells or C cells of the thyroid gland. This hormone regulates calcium and phosphate homeostasis in the body. It reduces blood calcium levels by inhibiting osteoclast activity in the bones and decreasing reabsorption in the kidneys leading to more calcium excretion. Calcitonin also interacts with its receptor a G protein-coupled receptor found in tissues like bone kidney and brain.

Biological function summary

This hormone plays a significant role in maintaining calcium levels by balancing the actions of parathyroid hormone (PTH). Calcitonin works independently but interacts with the broader system managing bone remodeling through an intricate network. The presence of calcitonin in specialized receptor complexes allows it to exert its effects efficiently in target tissues making it an important player in calcium and phosphorus metabolism.

Pathways

Calcitonin acts within the calcium signaling pathway and the bone resorption pathway. Through these pathways calcitonin works alongside other proteins such as parathyroid hormone (PTH) and calcitonin gene-related peptide (CGRP) maintaining skeletal integrity. It modulates the activity of osteoclasts ensuring that bone resorption activities are counterbalanced by bone formation and influences intracellular calcium concentration by binding to its receptor on target cells.

Calcitonin is linked to conditions such as osteoporosis and medullary thyroid carcinoma. Osteoporosis characterized by decreased bone density and increased fracture risk involves dysregulated calcium and phosphate balance. Proteins like estrogen and growth factors along with calcitonin influence bone health. Medullary thyroid carcinoma a type of thyroid cancer arising from C cells affects calcitonin levels with this hormone serving as a biomarker for diagnosing and monitoring treatment effectiveness. Calcitonin's connection with these diseases highlights its diagnostic and therapeutic potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed : 35324283).. Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.

See full target information CALCA

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 25:193 PubMed39516766

2024

Sex differences in expression of CGRP family of receptors and ligands in the rat trigeminal system.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Maddahi,Jacob C A Edvinsson,Lars Edvinsson

Nature communications 15:5555 PubMed39030177

2024

The neurotransmitter calcitonin gene-related peptide shapes an immunosuppressive microenvironment in medullary thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yingtong Hou,Bo Lin,Tianyi Xu,Juan Jiang,Shuli Luo,Wanna Chen,Xinwen Chen,Yuanqi Wang,Guanrui Liao,Jianping Wang,Jiayuan Zhang,Xuyang Li,Xiao Xiang,Yubin Xie,Ji Wang,Sui Peng,Weiming Lv,Yihao Liu,Haipeng Xiao

Journal of extracellular vesicles 13:e12413 PubMed38353485

2024

Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk.

Applications

Unspecified application

Species

Unspecified reactive species

Miira M Klemetti,Ante B V Pettersson,Aafaque Ahmad Khan,Leonardo Ermini,Tyler R Porter,Michael L Litvack,Sruthi Alahari,Stacy Zamudio,Nicholas P Illsley,Hannes Röst,Martin Post,Isabella Caniggia

Endocrine journal 67:1007-1017 PubMed32522910

2020

False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Takeshima,Hiroyuki Ariyasu,Shinsuke Uraki,Shuhei Morita,Yasushi Furukawa,Hidefumi Inaba,Hiroshi Iwakura,Asako Doi,Kenji Warigaya,Shin-Ichi Murata,Keisuke Enomoto,Muneki Hotomi,Takashi Akamizu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calcitonin antibody [SP17]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (AB16697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (AB16697)

Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin antibody [SP17] (AB16697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (AB16697)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise