AB230500

Anti-Calcitonin receptor/CT-R antibody

Name: Anti-Calcitonin receptor/CT-R antibody (ab230500) | Abcam
Brand: Abcam Limited
SKU: AB230500
Price: 595 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal Calcitonin receptor/CT-R antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CALCR aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Calcitonin receptor, CT-R, CALCR

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin receptor/CT-R antibody (AB230500)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin receptor/CT-R antibody (AB230500)

MCF7 (human breast adenocarcinoma cell line) cells labelling calcitonin using ab230500 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) in ICC/IF.

Nuclear counterstain : DAPI (blue).

Flow Cytometry (Intracellular) - Anti-Calcitonin receptor/CT-R antibody (AB230500)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calcitonin receptor/CT-R antibody (AB230500)

Intracellular flow cytometric analysis of calcitonin in HL-60 (human promyelocytic leukemia cell line) cells using ab230500 at 1/10 dilution (right histogram) compared to the negetive contolr (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody (AB230500)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody (AB230500)

Formalin-fixed, paraffin-embedded human colon (vessels) tissue stained for calcitonin using ab230500 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Calcitonin receptor/CT-R antibody (AB230500)

All lanes:

Western blot - Anti-Calcitonin receptor/CT-R antibody (ab230500) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) cell lysate without peptide (-) at 35 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) cell lysate with peptide (+) at 35 µg

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate without peptide (-) at 35 µg

Lane 4:

A431 (human epidermoid carcinoma cell line) cell lysate with peptide (+) at 35 µg

Secondary

All lanes:

goat anti-rabbit(HRP) at 1/5000 dilution

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CALCR aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P30988

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcitonin receptor (CT-R) is a protein that mechanically binds calcitonin a hormone involved in regulating calcium levels. Another known name is Salmon Calcitonin Receptor. The receptor has a mass approximately 70 kDa and is expressed in various tissues including kidney osteoclasts and the central nervous system. The receptor is a critical part of calcium-phosphate homeostasis facilitating the actions of calcitonin across different physiological systems in the body.

Biological function summary

CT-R serves a significant role in bone metabolism and mineral homeostasis. It participates in the inhibition of osteoclast-mediated bone resorption which is an important process in maintaining bone density. Although CT-R functions independently interactions with other hormones like parathyroid hormone and vitamin D highlight its importance in a larger network of bone health. The receptor contributes to reducing blood calcium levels by decreasing calcium release from bones and enhancing renal calcium excretion.

Pathways

CT-R is actively involved in the cyclic adenosine monophosphate (cAMP) and phosphoinositide signaling pathways essential for mediating cellular responses. Through these pathways CT-R closely associates with the related protein Calcitonin Gene-Related Peptide (CGRP) which shares some signaling components and effects on targeted cells. These pathways highlight the mechanistic basis for CT-R's roles in regulating calcium and phosphate metabolism.

CT-R has connections to osteoporosis and paget's disease. These conditions involve impaired bone metabolism where CT-R activity—or lack of activity—contributes to pathogenesis. The receptor's relationship with the proteins parathyroid hormone and CGRP suggests its indirect involvement in disease progression since these proteins also modulate bone health and are potential therapeutic targets for treating such bone disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

G protein-coupled receptor activated by ligand peptides amylin (IAPP), calcitonin (CT/CALCA) and calcitonin gene-related peptide type 1 (CGRP1/CALCA) (PubMed : 35324283, PubMed : 38603770). CALCR interacts with receptor-activity-modifying proteins RAMP1, 2 and 3 to form receptor complexes AMYR1, 2 and 3, respectively (PubMed : 35324283, PubMed : 38603770). IAPP, CT and CGRP1 activate CALCR and AMYRs with distinct modes of receptor activation resulting in specific phenotypes (PubMed : 35324283, PubMed : 38603770). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway (PubMed : 35324283, PubMed : 7476993).. Isoform 2. Non-functional protein. Unable to couple to G proteins and activate adenylyl cyclase (PubMed : 7476993). Does not undergo receptor internalization following ligand binding (PubMed : 7476993).

See full target information CALCR

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:10765-10783 PubMed38985127

2024

CALCR exacerbates renal cell carcinoma progression via stabilizing CD44.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyang Yan,Zhaohui Xing,Shuai Liu,Peng Gao,Qingli Wang,Guiying Guo

Brain : a journal of neurology 147:2128-2143 PubMed38226680

2024

Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Han,Kui Chen,Hongxiang Yu,Can Cui,Hongxia Li,Yongbo Hu,Bei Zhang,Gang Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calcitonin receptor/CT-R antibody

Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin receptor/CT-R antibody (AB230500)

Flow Cytometry (Intracellular) - Anti-Calcitonin receptor/CT-R antibody (AB230500)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody (AB230500)

Western blot - Anti-Calcitonin receptor/CT-R antibody (AB230500)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise