Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335) is a rabbit monoclonal antibody that is used to detect Calcitonin receptor/CT-R in Western Blot, IHC-P. Suitable for Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417751.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335)")
![Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--western-blot-img417730.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335)")
![Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--western-blot-img417729.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417752.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417753.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (AB313335)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Rat | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Transfected cell lysate - Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate - Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse, Transfected cell lysate - Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
G protein-coupled receptor activated by ligand peptides amylin (IAPP), calcitonin (CT/CALCA) and calcitonin gene-related peptide type 1 (CGRP1/CALCA). CALCR interacts with receptor-activity-modifying proteins RAMP1, 2 and 3 to form receptor complexes AMYR1, 2 and 3, respectively. IAPP, CT and CGRP1 activate CALCR and AMYRs with distinct modes of receptor activation resulting in specific phenotypes. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway.
Alternative names
Calcitonin receptor, CT-R, C1A/C1B, Calcr
Recommended products
Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335) is a rabbit monoclonal antibody that is used to detect Calcitonin receptor/CT-R in Western Blot, IHC-P. Suitable for Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27455-47
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Rat samples.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR27455-47] also available for your convenience:
ab313335, Carrier free - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free ab313336
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcitonin receptor (CT-R) is a protein that mechanically binds calcitonin a hormone involved in regulating calcium levels. Another known name is Salmon Calcitonin Receptor. The receptor has a mass approximately 70 kDa and is expressed in various tissues including kidney osteoclasts and the central nervous system. The receptor is a critical part of calcium-phosphate homeostasis facilitating the actions of calcitonin across different physiological systems in the body.
- Biological function summary
CT-R serves a significant role in bone metabolism and mineral homeostasis. It participates in the inhibition of osteoclast-mediated bone resorption which is an important process in maintaining bone density. Although CT-R functions independently interactions with other hormones like parathyroid hormone and vitamin D highlight its importance in a larger network of bone health. The receptor contributes to reducing blood calcium levels by decreasing calcium release from bones and enhancing renal calcium excretion.
- Pathways
CT-R is actively involved in the cyclic adenosine monophosphate (cAMP) and phosphoinositide signaling pathways essential for mediating cellular responses. Through these pathways CT-R closely associates with the related protein Calcitonin Gene-Related Peptide (CGRP) which shares some signaling components and effects on targeted cells. These pathways highlight the mechanistic basis for CT-R's roles in regulating calcium and phosphate metabolism.
- Associated diseases and disorders
CT-R has connections to osteoporosis and paget's disease. These conditions involve impaired bone metabolism where CT-R activity—or lack of activity—contributes to pathogenesis. The receptor's relationship with the proteins parathyroid hormone and CGRP suggests its indirect involvement in disease progression since these proteins also modulate bone health and are potential therapeutic targets for treating such bone disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417751.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)
Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling Calcitonin receptor/CT-R with ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat hypothalamus. (A) Low-powered (magnification, x20) and (B) high-powered (magnification, x200) microscopic images. The section was incubated with ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--western-blot-img417730.jpg "Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)")
Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with rat Calcitonin receptor-like receptor.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Exposure time: 6 secondsAll lanes: Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 2: 293T transfected with a rat Calcr (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 3: 293T transfected with a rat Calcrl (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55-70 kDa
Exposure time: 6s
![Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--western-blot-img417729.jpg "Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)")
Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: small intestine, thymus (PMID: 12730726).
The expression profile observed is consistent with what has been described in the literature (PMID: 8395656).
The identity of the higher MW bands at approximately 110 kDa and 120 kDa are unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 114 secondsAll lanes: Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335) at 1/1000 dilution
Lane 1: Rat E15 brain tissue lysate at 20 µg
Lane 2: Rat hypothalamus tissue lysate at 20 µg
Lane 3: Rat small intestine tissue lysate at 20 µg
Lane 4: Rat thymus tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 55-70 kDa
Exposure time: 114s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417752.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)
Immunohistochemical analysis of paraffin-embedded Rat thymus tissue labeling Calcitonin receptor/CT-R with ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat thymus. The section was incubated with ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335), expandable thumbnail](https://content.abcam.com/products/images/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417753.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (ab313335)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Calcitonin receptor/CT-R with ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat cardiac muscle. The section was incubated with ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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