Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336)

This data was developed using ab313335, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Calcitonin receptor/CT-R with ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat cardiac muscle. The section was incubated with ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336)

This data was developed using ab313335, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat thymus tissue labeling Calcitonin receptor/CT-R with ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat thymus. The section was incubated with ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336)

Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling Calcitonin receptor/CT-R with ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat hypothalamus. (A) Low-powered (magnification, x20) and (B) high-powered (magnification, x200) microscopic images. The section was incubated with ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336)

This data was developed using ab313335, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : small intestine, thymus (PMID : 12730726). The expression profile observed is consistent with what has been described in the literature (PMID : 8395656). The identity of the higher MW bands at approximately 110 kDa and 120 kDa are unknown. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 114 seconds

All lanes:

Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (<a href='/en-us/products/primary-antibodies/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335'>ab313335</a>) at 1/1000 dilution

Lane 1:

Rat E15 brain tissue lysate at 20 µg

Lane 2:

Rat hypothalamus tissue lysate at 20 µg

Lane 3:

Rat small intestine tissue lysate at 20 µg

Lane 4:

Rat thymus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55-70 kDa

false

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336)

This data was developed using ab313335, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with rat Calcitonin receptor-like receptor. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 6 seconds

All lanes:

Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (<a href='/en-us/products/primary-antibodies/calcitonin-receptor-ct-r-antibody-epr27455-47-ab313335'>ab313335</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with a rat Calcr (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with a rat Calcrl (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55-70 kDa

false

Exposure time: 6s