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Rabbit Recombinant Monoclonal Calcitonin receptor/CT-R antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Transfected cell lysate - Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336), expandable thumbnail
  • Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336), expandable thumbnail
  • Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (AB313336), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow CytIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Rat
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Transfected cell lysate - Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse, Transfected cell lysate - Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

G protein-coupled receptor activated by ligand peptides amylin (IAPP), calcitonin (CT/CALCA) and calcitonin gene-related peptide type 1 (CGRP1/CALCA). CALCR interacts with receptor-activity-modifying proteins RAMP1, 2 and 3 to form receptor complexes AMYR1, 2 and 3, respectively. IAPP, CT and CGRP1 activate CALCR and AMYRs with distinct modes of receptor activation resulting in specific phenotypes. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway.

Alternative names

Calcitonin receptor, CT-R, C1A/C1B, Calcr

Recommended products

Rabbit Recombinant Monoclonal Calcitonin receptor/CT-R antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Transfected cell lysate - Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27455-47
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Calcitonin receptor (CT-R) is a protein that mechanically binds calcitonin a hormone involved in regulating calcium levels. Another known name is Salmon Calcitonin Receptor. The receptor has a mass approximately 70 kDa and is expressed in various tissues including kidney osteoclasts and the central nervous system. The receptor is a critical part of calcium-phosphate homeostasis facilitating the actions of calcitonin across different physiological systems in the body.

Biological function summary

CT-R serves a significant role in bone metabolism and mineral homeostasis. It participates in the inhibition of osteoclast-mediated bone resorption which is an important process in maintaining bone density. Although CT-R functions independently interactions with other hormones like parathyroid hormone and vitamin D highlight its importance in a larger network of bone health. The receptor contributes to reducing blood calcium levels by decreasing calcium release from bones and enhancing renal calcium excretion.

Pathways

CT-R is actively involved in the cyclic adenosine monophosphate (cAMP) and phosphoinositide signaling pathways essential for mediating cellular responses. Through these pathways CT-R closely associates with the related protein Calcitonin Gene-Related Peptide (CGRP) which shares some signaling components and effects on targeted cells. These pathways highlight the mechanistic basis for CT-R's roles in regulating calcium and phosphate metabolism.

Associated diseases and disorders

CT-R has connections to osteoporosis and paget's disease. These conditions involve impaired bone metabolism where CT-R activity—or lack of activity—contributes to pathogenesis. The receptor's relationship with the proteins parathyroid hormone and CGRP suggests its indirect involvement in disease progression since these proteins also modulate bone health and are potential therapeutic targets for treating such bone disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336)

    Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling Calcitonin receptor/CT-R with Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat hypothalamus. (A) Low-powered (magnification, x20) and (B) high-powered (magnification, x200) microscopic images. The section was incubated with Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336), expandable thumbnail

    Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336)

    This data was developed using Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with rat Calcitonin receptor-like receptor.
    Samples are non-boiled as boiling may cause protein aggregation.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Exposure time: 6 seconds

    All lanes: Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 2: 293T transfected with a rat Calcr (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 3: 293T transfected with a rat Calcrl (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 55-70 kDa

    Exposure time: 6s

  • Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336), expandable thumbnail

    Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336)

    This data was developed using Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: small intestine, thymus (PMID: 12730726).
    The expression profile observed is consistent with what has been described in the literature (PMID: 8395656).
    The identity of the higher MW bands at approximately 110 kDa and 120 kDa are unknown.
    Samples are non-boiled as boiling may cause protein aggregation.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 114 seconds

    All lanes: Western blot - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] (Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335) at 1/1000 dilution

    Lane 1: Rat E15 brain tissue lysate at 20 µg

    Lane 2: Rat hypothalamus tissue lysate at 20 µg

    Lane 3: Rat small intestine tissue lysate at 20 µg

    Lane 4: Rat thymus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55-70 kDa

    Exposure time: 114s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336)

    This data was developed using Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat thymus tissue labeling Calcitonin receptor/CT-R with Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat thymus. The section was incubated with Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] - BSA and Azide free (ab313336)

    This data was developed using Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Calcitonin receptor/CT-R with Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat cardiac muscle. The section was incubated with Anti-Calcitonin receptor/CT-R antibody [EPR27455-47] ab313335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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