Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] KO Tested (ab313763)

Knockout Tested Rabbit Recombinant Monoclonal Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (AB313763), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	IP	Flow Cyt
Human	Tested	Tested	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500 - 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500 - 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500 - 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Cacna2d1

Function

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel (By similarity). Plays an important role in excitation-contraction coupling (By similarity).

Alternative names

Cacna2, Cacna2d1, Voltage-dependent calcium channel subunit alpha-2/delta-1, Voltage-gated calcium channel subunit alpha-2/delta-1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23267-59
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Calcium channel L type DHPR alpha 2 subunit also known as CACNA2D1 or alpha-2-delta plays a significant role in cellular calcium regulation. This subunit typically part of the voltage-gated calcium channel complex allows calcium ions to enter the cells following electrical stimuli. With a mass of about 150 kDa CACNA2D1 expresses predominantly in skeletal and cardiac muscle tissues and the central nervous system. Its presence across different tissues suggests various physiological roles linking its mechanical function to electrical signaling and muscle contraction.

Biological function summary

CACNA2D1 aids in modulating neurotransmitter release and excitation-contraction coupling. This protein often integrates into the larger voltage-gated calcium channel complex working in concert with other subunits to optimize calcium flow. This particular function supports critical processes in muscle cells and neurons integrating signals in response to electrical impulses. The alpha-2-delta ligand poses significance here as well often used to modify the channel's activity and study its role in cellular communication.

Pathways

CACNA2D1 actively participates in electrical signaling and muscle contraction pathways. It contributes to the excitation-contraction coupling pathway in muscle cells aligning closely with proteins like the ryanodine receptor ensuring efficient calcium-induced calcium release. Its action also extends to the neurotransmitter release pathway where it associates with synaptic proteins regulating neurotransmission. The function within these pathways underlines its importance in both muscle and neural activities.

Associated diseases and disorders

CACNA2D1 associates with conditions such as epilepsy and neuropathic pain. The dysfunction in its channel activity can lead to altered calcium flow contributing to these disorders. The alpha-2-delta ligand such as the gabapentin chemical structure plays a role here often targeting the channel to mitigate symptoms. Gabapentin's influence highlights the therapeutic potential of targeting CACNA2D1 in disease management providing critical insights into neuromodulation treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: NR8383
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: GH3(rat pituitary epithelial cell) whole cell lysate at 20 µg
Lane 3: NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa
Exposure time: 15s
Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: lung (PMID:8955374)
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa
Exposure time: 15s
Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: lung (PMID:8955374)
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa
Exposure time: 15s
Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: THP-1

Low expression: Neuro-2a(PMID: 30404013).

Performed under reducing conditions.

In Western blot, ab313763 was shown to bind specifically to CACNA2D1.

Target of interest was observed at 150 kDa in wild-type HeLa cell lysates (lane 1, wild-type cell line ab255928) with no signal observed at this size in CACNA2D1 knockout cell line (lane 2, knockout cell line Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell line ab265080/ knockout cell lysate Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate ab257374).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 37 seconds
All lanes: Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: CACNA2D1 knockout HeLa whole cell lysate at 20 µg
Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 6: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Lane 7: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 8: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 150 kDa
Exposure time: 37s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Immunohistochemical analysis of paraffin-embedded (A) Wild-type Hela ( tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab313763 at 1/500 (1.046 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) Wild-type Hela cell pellet; no staining on (B) CACNA2D1 knockout HeLa cell pellet and (C) THP-1 cell pellet. The section was incubated with ab313763 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab313763 at 1/4000 (0.131 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat skeletal muscle. The section was incubated with ab313763 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab313763 at 1/4000 (0.131 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse skeletal muscle (PMID: 18063658). The section was incubated with ab313763 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab313763 at 1/4000 (0.131 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cardiac muscle. The section was incubated with ab313763 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-59] (ab313763)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab313763 at 1/4000 (0.131 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skeletal muscle. The section was incubated with ab313763 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins