AB273881

Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Cacna2, Cacna2d1, Voltage-dependent calcium channel subunit alpha-2/delta-1, Voltage-gated calcium channel subunit alpha-2/delta-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse skeletal muscle (PMID : 18063658). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat skeletal muscle (PMID : 18063658). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat cerebrum (PMID : 8955374). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat pancreatic islet (PMID : 28115397). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).

Unconjugated

Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23267-8

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab273881 is the carrier-free version of ab253190.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Calcium channel L type DHPR alpha 2 subunit also known as CACNA2D1 or alpha-2-delta plays a significant role in cellular calcium regulation. This subunit typically part of the voltage-gated calcium channel complex allows calcium ions to enter the cells following electrical stimuli. With a mass of about 150 kDa CACNA2D1 expresses predominantly in skeletal and cardiac muscle tissues and the central nervous system. Its presence across different tissues suggests various physiological roles linking its mechanical function to electrical signaling and muscle contraction.

Biological function summary

CACNA2D1 aids in modulating neurotransmitter release and excitation-contraction coupling. This protein often integrates into the larger voltage-gated calcium channel complex working in concert with other subunits to optimize calcium flow. This particular function supports critical processes in muscle cells and neurons integrating signals in response to electrical impulses. The alpha-2-delta ligand poses significance here as well often used to modify the channel's activity and study its role in cellular communication.

Pathways

CACNA2D1 actively participates in electrical signaling and muscle contraction pathways. It contributes to the excitation-contraction coupling pathway in muscle cells aligning closely with proteins like the ryanodine receptor ensuring efficient calcium-induced calcium release. Its action also extends to the neurotransmitter release pathway where it associates with synaptic proteins regulating neurotransmission. The function within these pathways underlines its importance in both muscle and neural activities.

CACNA2D1 associates with conditions such as epilepsy and neuropathic pain. The dysfunction in its channel activity can lead to altered calcium flow contributing to these disorders. The alpha-2-delta ligand such as the gabapentin chemical structure plays a role here often targeting the channel to mitigate symptoms. Gabapentin's influence highlights the therapeutic potential of targeting CACNA2D1 in disease management providing critical insights into neuromodulation treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel (By similarity). Plays an important role in excitation-contraction coupling (By similarity).

See full target information Cacna2d1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (AB273881)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise