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Rabbit Recombinant Monoclonal Calcium-independent Phospholipase A2/PLA2G6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (AB259950), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (AB259950), expandable thumbnail
  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (AB259950), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (AB259950), expandable thumbnail
  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (AB259950), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFFlow CytWBIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles (PubMed:10092647, PubMed:10336645, PubMed:20886109, PubMed:9417066). Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position (PubMed:10092647, PubMed:10336645, PubMed:20886109). Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity) (PubMed:20886109). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content (PubMed:23533611). Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species (By similarity). Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs) (By similarity). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle (PubMed:20886109). Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin (By similarity). Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis (PubMed:18208975). Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types (By similarity). Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid (By similarity). In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion (By similarity). Isoform Ankyrin-iPLA2-1. Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. Isoform Ankyrin-iPLA2-2. Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms.

Alternative names

PLPLA9, PLA2G6, 85/88 kDa calcium-independent phospholipase A2, CaI-PLA2, 2-lysophosphatidylcholine acylhydrolase, Group VI phospholipase A2, Intracellular membrane-associated calcium-independent phospholipase A2 beta, Palmitoyl-CoA hydrolase, Patatin-like phospholipase domain-containing protein 9, GVI PLA2, iPLA2-beta, PNPLA9

Recommended products

Rabbit Recombinant Monoclonal Calcium-independent Phospholipase A2/PLA2G6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23994-103
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein Calcium-independent Phospholipase A2 also known as iPLA2 or PLA2G6 is an important enzyme involved in phospholipid metabolism. This enzyme exhibits a catalytic function that does not require calcium for activation which distinguishes it from other members of the phospholipase A2 family. It possesses a molecular weight of approximately 85 kDa. This protein mainly expresses in cytosolic compartments within several tissues including the heart brain and pancreas playing a diverse role in cellular activities.

Biological function summary

The protein catalyzes the hydrolysis of ester bonds within phospholipids releasing free fatty acids and lysophospholipids. This enzyme does not operate as part of a larger complex but functions independently within the cell membrane and metabolic pathways. By regulating phospholipid turnover and remodeling the protein plays a vital role in membrane homeostasis and signaling. This remodeling contributes to cell survival and apoptosis depending on cellular needs and conditions.

Pathways

This enzyme interacts with lipid signaling and metabolism in central biological processes. It plays a significant role in the glycerophospholipid and arachidonic acid pathways. In these pathways iPLA2 is closely related to cyclooxygenase and lipoxygenase enzymes which convert arachidonic acid into prostaglandins and leukotrienes leading to the mediation of inflammation and other signaling pathways. This interaction emphasizes the enzyme's importance in both normal physiology and pathophysiological conditions.

Associated diseases and disorders

Mutations or altered activities of iPLA2 have associations with neurodegenerative disorders such as Parkinson’s disease and infantile neuroaxonal dystrophy. In these disorders mitochondrial dysfunction often linked with proteins like alpha-synuclein becomes prevalent demonstrating the enzyme's influence on apoptosis and oxidative stress. Furthermore iPLA2 mutations may lead to cardiomyopathy where its function overlaps with creatine kinase and enzymes involved in energy metabolism indicating a broader impact on cardiac health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 29109765)

    Low expression: brain, lung (PMID:10336645)

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Mouse lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa

    Observed band size: 36 kDa, 88 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:29109765)

    Low expression: brain, lung (PMID:10336645)

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1: Rat testis tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Lane 3: Rat lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa

    Observed band size: 36 kDa, 88 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on rat testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa

    Observed band size: 88 kDa

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    Exposure time: 5.5 seconds.

    Negative control: human liver and human colon.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/20, 0000 dilution.

    All lanes: Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 40 µg

    Lane 2: Human liver tissue lysate at 40 µg

    Lane 3: Human colon tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 89 kDa

    Observed band size: 89 kDa

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    Negative control: PC-3.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/20, 0000 dilution.

    All lanes: Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1: LNCaP (human prostate carcinoma epithelial cell) while cell lysate at 20 µg

    Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) while cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 89 kDa

    Observed band size: 89 kDa

    Exposure time: 15s

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950), expandable thumbnail

    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Calcium-independent Phospholipase A2/PLA2G6 Western blot staining using rabbit Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody

    Western blot: Rabbit Monoclonal[EPR23994-103] to Calcium-independent Phospholipase A2/PLA2G6 ab259950 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 76 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PLA2G6 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG at 20 µg

    Lane 2: Western blot - Human PLA2G6 knockout U-87 MG cell line (Human PLA2G6 knockout U-87 MG cell line ab306752) at 20 µg

    Lane 3: LNCaP at 20 µg

    Lane 4: Human Brain at 20 µg

    Lane 5: PC-3 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 90 kDa

    Observed band size: 76 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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