Skip to main content

Rabbit Recombinant Monoclonal Calcium-independent Phospholipase A2/PLA2G6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (AB278086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (AB278086), expandable thumbnail
  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (AB278086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (AB278086), expandable thumbnail
  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (AB278086), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-FrIHC-PIP
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles (PubMed:10092647, PubMed:10336645, PubMed:20886109, PubMed:9417066). Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position (PubMed:10092647, PubMed:10336645, PubMed:20886109). Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity) (PubMed:20886109). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content (PubMed:23533611). Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species (By similarity). Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs) (By similarity). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle (PubMed:20886109). Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin (By similarity). Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis (PubMed:18208975). Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types (By similarity). Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid (By similarity). In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion (By similarity). Isoform Ankyrin-iPLA2-1. Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. Isoform Ankyrin-iPLA2-2. Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms.

Alternative names

PLPLA9, PLA2G6, 85/88 kDa calcium-independent phospholipase A2, CaI-PLA2, 2-lysophosphatidylcholine acylhydrolase, Group VI phospholipase A2, Intracellular membrane-associated calcium-independent phospholipase A2 beta, Palmitoyl-CoA hydrolase, Patatin-like phospholipase domain-containing protein 9, GVI PLA2, iPLA2-beta, PNPLA9

Recommended products

Rabbit Recombinant Monoclonal Calcium-independent Phospholipase A2/PLA2G6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR23994-103
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab278086 is the carrier-free version of Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] ab259950.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein Calcium-independent Phospholipase A2 also known as iPLA2 or PLA2G6 is an important enzyme involved in phospholipid metabolism. This enzyme exhibits a catalytic function that does not require calcium for activation which distinguishes it from other members of the phospholipase A2 family. It possesses a molecular weight of approximately 85 kDa. This protein mainly expresses in cytosolic compartments within several tissues including the heart brain and pancreas playing a diverse role in cellular activities.

Biological function summary

The protein catalyzes the hydrolysis of ester bonds within phospholipids releasing free fatty acids and lysophospholipids. This enzyme does not operate as part of a larger complex but functions independently within the cell membrane and metabolic pathways. By regulating phospholipid turnover and remodeling the protein plays a vital role in membrane homeostasis and signaling. This remodeling contributes to cell survival and apoptosis depending on cellular needs and conditions.

Pathways

This enzyme interacts with lipid signaling and metabolism in central biological processes. It plays a significant role in the glycerophospholipid and arachidonic acid pathways. In these pathways iPLA2 is closely related to cyclooxygenase and lipoxygenase enzymes which convert arachidonic acid into prostaglandins and leukotrienes leading to the mediation of inflammation and other signaling pathways. This interaction emphasizes the enzyme's importance in both normal physiology and pathophysiological conditions.

Associated diseases and disorders

Mutations or altered activities of iPLA2 have associations with neurodegenerative disorders such as Parkinson’s disease and infantile neuroaxonal dystrophy. In these disorders mitochondrial dysfunction often linked with proteins like alpha-synuclein becomes prevalent demonstrating the enzyme's influence on apoptosis and oxidative stress. Furthermore iPLA2 mutations may lead to cardiomyopathy where its function overlaps with creatine kinase and enzymes involved in energy metabolism indicating a broader impact on cardiac health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com