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AB3529

Anti-Calcium Pump PMCA2 ATPase antibody

5

(3 Reviews)

|

(15 Publications)

Rabbit Polyclonal Calcium Pump PMCA2 ATPase antibody. Suitable for ICC, IP, ELISA, WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Pig, Chicken, Primates, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ATP2B2 aa 1-50.

View Alternative Names

PMCA2, ATP2B2, Plasma membrane calcium-transporting ATPase 2, Plasma membrane calcium ATPase isoform 2, Plasma membrane calcium pump isoform 2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (AB3529)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (AB3529)

Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (AB3529)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (AB3529)

Human cerebellum (Centre panel) and lung (Negative control, right panel) stained for PMCA2 ATPase using ab3529 at 1/20 dilution in immunohistochemical analysis. The left hand panel is a secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (AB3529)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (AB3529)

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 20 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Primates, Chicken, Mouse, Pig, Rat

Applications

ICC, IP, WB, ELISA, IHC-P, ICC/IF, IHC-Fr, IHC-FoFr

applications

Immunogen

Synthetic Peptide within Human ATP2B2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q01814

Specificity

Detects PMCA 2a and 2b ATPase.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Calcium Pump PMCA2 ATPase also known as PMCA2 or Ca-ATPase pump plays an important role in the active transport of calcium ions from the cytoplasm across the plasma membrane. It functions by hydrolyzing ATP to provide the energy needed for this process. PMCA2 belongs to the family of P-type ATPases and has a molecular mass of approximately 135 kDa. This pump is prominently expressed in the brain and inner ear particularly in the cerebellum and cochlea highlighting its importance in neuronal and auditory functions.
Biological function summary

PMCA2 regulates intracellular calcium concentration which is critical for cellular signaling and homeostasis. It does not form part of a larger complex but works independently to balance calcium levels. In neuronal cells efficient calcium clearance is necessary for synaptic activity and PMCA2 helps in maintaining the rapid calcium ion flux during neuronal firing. The presence of PMCA2 in auditory hair cells signifies its role in cochlear function and hearing sensitivity by aiding the processing of sound signals through calcium modulation.

Pathways

PMCA2 integrates into calcium signaling pathways and interacts with calmodulin which enhances its activity. Its function in the calmodulin-calcium signaling pathway is essential for processes such as neurotransmitter release and muscle contraction. In association with proteins like calmodulin PMCA2 ensures precise calcium dynamics safeguarding against cytotoxicity triggered by excessive calcium accumulation. The coordination with calmodulin formulates a pivotal connection within various signaling cascades.

PMCA2 mutations or dysfunctions have associations with neurological disorders and hearing impairments. Research links PMCA2 dysregulation to conditions such as spinocerebellar ataxia and hereditary hearing loss. In these scenarios PMCA2 interacts with molecular components that when impaired disrupt the normal calcium homeostasis. Such interruptions can lead to neurodegeneration or compromised auditory function underlining the significance of PMCA2 in maintaining health and preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels in specialized cells of cerebellar circuit and vestibular and cochlear systems (PubMed : 15829536, PubMed : 17234811). Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment (PubMed : 15829536, PubMed : 17234811). Has fast activation and Ca(2+) clearance rate suited to control fast neuronal Ca(2+) dynamics. At parallel fiber to Purkinje neuron synapse, mediates presynaptic Ca(2+) efflux in response to climbing fiber-induced Ca(2+) rise. Provides for fast return of Ca(2+) concentrations back to their resting levels, ultimately contributing to long-term depression induction and motor learning (By similarity). Plays an essential role in hearing and balance (PubMed : 15829536, PubMed : 17234811). In cochlear hair cells, shuttles Ca(2+) ions from stereocilia to the endolymph and dissipates Ca(2+) transients generated by the opening of the mechanoelectrical transduction channels. Regulates Ca(2+) levels in the vestibular system, where it contributes to the formation of otoconia (PubMed : 15829536, PubMed : 17234811). In non-excitable cells, regulates Ca(2+) signaling through spatial control of Ca(2+) ions extrusion and dissipation of Ca(2+) transients generated by store-operated channels (PubMed : 25690014). In lactating mammary gland, allows for the high content of Ca(2+) ions in the milk (By similarity).
See full target information ATP2B2
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40227213

2025

Large Fibrous Connective Tissue Reduces Oxidative Stress to Form a Living Cell Scaffold in Adipose Grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Yue,Zilong Cao,Tiran Zhang,Ningbei Yin,Liqiang Liu

BMC biology 20:67 PubMed35296311

2022

Identification and characterisation of spontaneous mutations causing deafness from a targeted knockout programme.

Applications

Unspecified application

Species

Unspecified reactive species

Morag A Lewis,Neil J Ingham,Jing Chen,Selina Pearson,Francesca Di Domenico,Sohinder Rekhi,Rochelle Allen,Matthew Drake,Annelore Willaert,Victoria Rook,Johanna Pass,Thomas Keane,David J Adams,Abigail S Tucker,Jacqueline K White,Karen P Steel

PLoS genetics 18:e1009937 PubMed35100259

2022

Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing.

Applications

Unspecified application

Species

Unspecified reactive species

Sherylanne Newton,Fanbo Kong,Adam J Carlton,Carlos Aguilar,Andrew Parker,Gemma F Codner,Lydia Teboul,Sara Wells,Steve D M Brown,Walter Marcotti,Michael R Bowl

International journal of molecular sciences 22: PubMed34948386

2021

Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Katarina Ilic,Xiao Lin,Ayse Malci,Mario Stojanović,Borna Puljko,Marko Rožman,Željka Vukelić,Marija Heffer,Dirk Montag,Ronald L Schnaar,Svjetlana Kalanj-Bognar,Rodrigo Herrera-Molina,Kristina Mlinac-Jerkovic

Life science alliance 5: PubMed34815294

2021

MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Kuu Ikäheimo,Anni Herranen,Vilma Iivanainen,Tuuli Lankinen,Antti A Aarnisalo,Ville Sivonen,Kashyap A Patel,Korcan Demir,Mart Saarma,Maria Lindahl,Ulla Pirvola

Neuron 107:126-143.e8 PubMed32343945

2020

TMIE Defines Pore and Gating Properties of the Mechanotransduction Channel of Mammalian Cochlear Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher L Cunningham,Xufeng Qiu,Zizhen Wu,Bo Zhao,Guihong Peng,Ye-Hyun Kim,Amanda Lauer,Ulrich Müller

Neuron 96:827-838.e9 PubMed29056295

2017

Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca-ATPases and Key Regulators of Ca Clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Schmidt,Astrid Kollewe,Cristina E Constantin,Sebastian Henrich,Andreas Ritzau-Jost,Wolfgang Bildl,Anja Saalbach,Stefan Hallermann,Akos Kulik,Bernd Fakler,Uwe Schulte

Frontiers in physiology 8:336 PubMed28588505

2017

Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells.

Applications

WB

Species

Unspecified reactive species

Sarah Y T Robertson,Xin Wen,Kaifeng Yin,Junjun Chen,Charles E Smith,Michael L Paine

Journal of animal science and biotechnology 7:41 PubMed27471592

2016

1,25-Dihydroxyvitamin D3 modulates calcium transport in goat mammary epithelial cells in a dose- and energy-dependent manner.

Applications

WB

Species

Goat

Feifei Sun,Yangchun Cao,Chao Yu,Xiaoshi Wei,Junhu Yao

Clinical proteomics 12:27 PubMed26604869

2015

Label-free mass spectrometric analysis reveals complex changes in the brain proteome from the mdx-4cv mouse model of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Murphy,Margit Zweyer,Michael Henry,Paula Meleady,Rustam R Mundegar,Dieter Swandulla,Kay Ohlendieck
View all publications
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