Rabbit Polyclonal Calcium Pump PMCA3 ATPase antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Plasma membrane calcium-transporting ATPase 3 aa 550-900.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Plasma membrane calcium-transporting ATPase 3 aa 550-900. The exact immunogen used to generate this antibody is proprietary information. Database link Q16720
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels at the presynaptic terminals (PubMed:18029012, PubMed:22912398, PubMed:25953895, PubMed:27035656). Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment (PubMed:25953895, PubMed:27035656). May counter-transport protons, but the mechanism and the stoichiometry of this Ca(2+)/H(+) exchange remains to be established (By similarity).
Alternative names
Plasma membrane calcium-transporting ATPase 3, PMCA3, Plasma membrane calcium ATPase isoform 3, Plasma membrane calcium pump isoform 3, ATP2B3
Recommended products
Rabbit Polyclonal Calcium Pump PMCA3 ATPase antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Plasma membrane calcium-transporting ATPase 3 aa 550-900.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Plasma membrane calcium-transporting ATPase 3 aa 550-900. The exact immunogen used to generate this antibody is proprietary information. Database link Q16720
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Calcium Pump PMCA3 ATPase also known as Plasma Membrane Calcium-Transporting ATPase 3 plays an important role in calcium homeostasis. This enzyme weighing approximately 134 kDa actively transports calcium ions out of cells to maintain low intracellular calcium levels. PMCA3 is mainly expressed in the brain and other neural tissues where it helps regulate neuronal signaling. The pump uses ATP to move calcium against its concentration gradient from the cytosol to the extracellular space a critical function for cellular activities.
- Biological function summary
PMCA3 controls the concentration of cytosolic calcium ions which influences many cellular processes such as muscle contraction neurotransmitter release and gene expression. PMCA3 often functions independently although it can sometimes exist in complexes with other proteins influencing its regulation and activity. The regulation of neuronal calcium levels through PMCA3 is essential for proper synaptic function and plasticity making it a point of interest in neurophysiological research.
- Pathways
PMCA3 plays a role in the calcium signaling pathway affecting signal transduction processes across various cell types. It acts alongside other calcium-handling proteins including Ca2+ channels and buffers in modulating cellular responses to physiological stimuli. Additionally PMCA3 intersects with the MAPK signaling pathway by influencing intracellular calcium which in turn can activate protein kinases and affect transcription regulation.
- Associated diseases and disorders
PMCA3 holds relevance in conditions such as spinocerebellar ataxia and epilepsy. Mutations or dysregulation of PMCA3 can disrupt calcium homeostasis leading to neurodegenerative conditions such as ataxia where coordination and balance are impaired. The dysregulated calcium flow can also influence seizure activity in epilepsy by affecting neurons' excitability. Associations with proteins like calmodulin which regulates PMCA3 activity further elucidate its pathophysiological connections in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab154213)
Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue, labeling Calcium Pump PMCA3 ATPase with ab154213 at 1/500 dilution.
Western blot - Anti-Calcium Pump PMCA3 ATPase antibody (ab154213)
5% SDS PAGE
All lanes: Western blot - Anti-Calcium Pump PMCA3 ATPase antibody (ab154213) at 1/1000 dilution
All lanes: U-87 MG whole cell lysate at 30 µg
Predicted band size: 134 kDa
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