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AB3530

Anti-Calcium Pump PMCA3 ATPase antibody

4

(3 Reviews)

|

(5 Publications)

Rabbit Polyclonal Calcium Pump PMCA3 ATPase antibody. Suitable for IHC, IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Plasma membrane calcium-transporting ATPase 3, PMCA3, Plasma membrane calcium ATPase isoform 3, Plasma membrane calcium pump isoform 3, ATP2B3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (AB3530)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (AB3530)

Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry - Anti-Calcium Pump PMCA3 ATPase antibody (AB3530)
  • IHC

Supplier Data

Immunohistochemistry - Anti-Calcium Pump PMCA3 ATPase antibody (AB3530)

Immunohistochemical analysis of liver and liver cancer tissue labelling Calcium Pump PMCA3 ATPase with ab3530. No staining observed in secondary antibody only control, and human tonsil which does not express Calcium Pump PMCA3 ATPase.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (AB3530)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (AB3530)

Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IHC

applications

Specificity

Detects PMCA 3a and 3b ATPase.

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Alternative Products

Primary Antibodies

AB154213

Anti-Calcium Pump PMCA3 ATPase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (AB154213)

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Complementary Products

Primary Antibodies

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Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151)

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Primary Antibodies

AB2783

Anti-Calcium Pump PMCA4 ATPase antibody [JA9]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (AB2783)

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Primary Antibodies

AB190355

Anti-PMCA1 antibody [EPR12029]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (AB190355)

  • 0
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "1/20", "IHC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Calcium Pump PMCA3 ATPase also known as Plasma Membrane Calcium-Transporting ATPase 3 plays an important role in calcium homeostasis. This enzyme weighing approximately 134 kDa actively transports calcium ions out of cells to maintain low intracellular calcium levels. PMCA3 is mainly expressed in the brain and other neural tissues where it helps regulate neuronal signaling. The pump uses ATP to move calcium against its concentration gradient from the cytosol to the extracellular space a critical function for cellular activities.
Biological function summary

PMCA3 controls the concentration of cytosolic calcium ions which influences many cellular processes such as muscle contraction neurotransmitter release and gene expression. PMCA3 often functions independently although it can sometimes exist in complexes with other proteins influencing its regulation and activity. The regulation of neuronal calcium levels through PMCA3 is essential for proper synaptic function and plasticity making it a point of interest in neurophysiological research.

Pathways

PMCA3 plays a role in the calcium signaling pathway affecting signal transduction processes across various cell types. It acts alongside other calcium-handling proteins including Ca2+ channels and buffers in modulating cellular responses to physiological stimuli. Additionally PMCA3 intersects with the MAPK signaling pathway by influencing intracellular calcium which in turn can activate protein kinases and affect transcription regulation.

PMCA3 holds relevance in conditions such as spinocerebellar ataxia and epilepsy. Mutations or dysregulation of PMCA3 can disrupt calcium homeostasis leading to neurodegenerative conditions such as ataxia where coordination and balance are impaired. The dysregulated calcium flow can also influence seizure activity in epilepsy by affecting neurons' excitability. Associations with proteins like calmodulin which regulates PMCA3 activity further elucidate its pathophysiological connections in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels at the presynaptic terminals (PubMed : 18029012, PubMed : 22912398, PubMed : 25953895, PubMed : 27035656). Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment (PubMed : 25953895, PubMed : 27035656). May counter-transport protons, but the mechanism and the stoichiometry of this Ca(2+)/H(+) exchange remains to be established (By similarity).
See full target information Plasma membrane calcium-transporting ATPase 3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 9: PubMed31877883

2019

Priority Strategy of Intracellular Ca Homeostasis in Skeletal Muscle Fibers During the Multiple Stresses of Hibernation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Xiaoyu Li,Fazeela Ismail,Shenhui Xu,Zhe Wang,Xin Peng,Chenxi Yang,Hui Chang,Huiping Wang,Yunfang Gao

Frontiers in physiology 8:336 PubMed28588505

2017

Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells.

Applications

WB

Species

Unspecified reactive species

Sarah Y T Robertson,Xin Wen,Kaifeng Yin,Junjun Chen,Charles E Smith,Michael L Paine

Disease models & mechanisms 9:553-62 PubMed27013529

2016

Spontaneous shaker rat mutant - a new model for X-linked tremor/ataxia.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Karla P Figueroa,Sharan Paul,Tito Calì,Raffaele Lopreiato,Sukanya Karan,Martina Frizzarin,Darren Ames,Ginevra Zanni,Marisa Brini,Warunee Dansithong,Brett Milash,Daniel R Scoles,Ernesto Carafoli,Stefan M Pulst

Biochemical and biophysical research communication 411:235-40 PubMed21740891

2011

Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: implications for catecholamine secretion by PC12 cells.

Applications

WB

Species

Rat

Michalina Kosiorek,Paulina Podszywalow-Bartnicka,Ludmila Zylinska,Krzysztof Zablocki,Slawomir Pikula

The Journal of physiology 579:85-99 PubMed17170045

2006

Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3.

Applications

WB

Species

Rat

Thomas P Jensen,Adelaida G Filoteo,Thomas Knopfel,Ruth M Empson
View all publications
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Product promise

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