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AB238158

Anti-Calcium Pump PMCA4 ATPase antibody

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(1 Publication)

Rabbit Polyclonal Calcium Pump PMCA4 ATPase antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP2B4 aa 250-350.

View Alternative Names

ATP2B2, MXRA1, ATP2B4, Plasma membrane calcium-transporting ATPase 4, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

Paraffin-embedded human colon cancer tissue stained for Calcium Pump PMCA4 ATPase with ab238158 at a 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

Paraffin-embedded human brain tissue stained for Calcium Pump PMCA4 ATPase with ab238158 at a 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Calcium Pump PMCA4 ATPase (Green) using ab238158 at a 1/33 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ATP2B4 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P23634

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Alternative Products

Primary Antibodies

AB2783

Anti-Calcium Pump PMCA4 ATPase antibody [JA9]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (AB2783)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB3530

Anti-Calcium Pump PMCA3 ATPase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (AB3530)

  • 4
  • 3
Primary Antibodies

AB190355

Anti-PMCA1 antibody [EPR12029]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (AB190355)

  • 0
  • 1
Primary Antibodies

AB319151

Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Calcium Pump PMCA4 ATPase also known as ATP2B4 is a plasma membrane calcium ATPase responsible for the extrusion of Ca2+ ions from the cell. It plays an essential role in maintaining calcium homeostasis within eukaryotic cells. PMCA4 weighs approximately 134 kDa and is expressed in various tissues including the heart brain and kidney. The protein actively transports Ca2+ out of the cell using energy obtained from ATP hydrolysis.
Biological function summary

PMCA4 regulates intracellular calcium levels impacting processes such as muscle contraction neurotransmitter release and cell signaling. PMCA4 does not function as part of a larger protein complex but interacts with calmodulin which modulates its activity. Calmodulin binding activates PMCA4 increasing its capability to efflux Ca2+ and maintain cellular calcium balance.

Pathways

The calcium pump PMCA4 ATPase plays a critical role in the calcium signaling pathway and the regulation of cardiac rhythm. It helps control Ca2+ concentration within the cell affecting proteins like calcineurin that respond to calcium fluctuations. PMCA4 also interrelates with the sodium-calcium exchanger integrally involved in cardiac muscle contraction and relaxation.

PMCA4 has been linked to conditions such as heart failure and certain neurological disorders. Abnormal expression or function of PMCA4 can dysregulate calcium levels contributing to cardiac arrhythmias. Additionally altered PMCA4 activity can affect synaptic function connecting it indirectly to conditions like schizophrenia. Its interaction with proteins like alpha-synuclein implicated in neurological disorders further highlights PMCA4's relevance in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell (PubMed : 8530416). By regulating sperm cell calcium homeostasis, may play a role in sperm motility (By similarity).
See full target information ATP2B4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cancer research and clinical oncology 150:294 PubMed38842683

2024

LncRNA HOXC-AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Fang Hou,Zhenghua Teng,Weiwei Xia,Jie Peng
View all publications
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Product promise

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