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Mouse Monoclonal Calcium Pump PMCA4 ATPase antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human, Cow samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ATP2B4.


Images

Flow Cytometry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (AB2783), expandable thumbnail
  • Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (AB2783), expandable thumbnail
  • Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (AB2783), expandable thumbnail
  • Immunocytochemistry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (AB2783), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (AB2783), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human ATP2B4. Database link P23634

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCFlow CytWB
Human
Tested
Tested
Tested
Tested
Cow
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

By Western blot, this antibody detects an ~129 kDa and an ~133 kDa protein representing PMCA4b and PMCA4a ATPase, respectively.

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell (PubMed:8530416). By regulating sperm cell calcium homeostasis, may play a role in sperm motility (By similarity).

Alternative names

ATP2B2, MXRA1, ATP2B4, Plasma membrane calcium-transporting ATPase 4, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4

Recommended products

Mouse Monoclonal Calcium Pump PMCA4 ATPase antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human, Cow samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ATP2B4.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Human ATP2B4. Database link P23634
Clone number
JA9
Purification technique
Affinity purification
Specificity

This antibody is specific to the PMCA4 isoform.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Calcium Pump PMCA4 ATPase also known as ATP2B4 is a plasma membrane calcium ATPase responsible for the extrusion of Ca2+ ions from the cell. It plays an essential role in maintaining calcium homeostasis within eukaryotic cells. PMCA4 weighs approximately 134 kDa and is expressed in various tissues including the heart brain and kidney. The protein actively transports Ca2+ out of the cell using energy obtained from ATP hydrolysis.

Biological function summary

PMCA4 regulates intracellular calcium levels impacting processes such as muscle contraction neurotransmitter release and cell signaling. PMCA4 does not function as part of a larger protein complex but interacts with calmodulin which modulates its activity. Calmodulin binding activates PMCA4 increasing its capability to efflux Ca2+ and maintain cellular calcium balance.

Pathways

The calcium pump PMCA4 ATPase plays a critical role in the calcium signaling pathway and the regulation of cardiac rhythm. It helps control Ca2+ concentration within the cell affecting proteins like calcineurin that respond to calcium fluctuations. PMCA4 also interrelates with the sodium-calcium exchanger integrally involved in cardiac muscle contraction and relaxation.

Associated diseases and disorders

PMCA4 has been linked to conditions such as heart failure and certain neurological disorders. Abnormal expression or function of PMCA4 can dysregulate calcium levels contributing to cardiac arrhythmias. Additionally altered PMCA4 activity can affect synaptic function connecting it indirectly to conditions like schizophrenia. Its interaction with proteins like alpha-synuclein implicated in neurological disorders further highlights PMCA4's relevance in disease states.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Flow Cytometry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783), expandable thumbnail

    Flow Cytometry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

    Overlay histogram showing HeLa cells stained with ab2783 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2783, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783), expandable thumbnail

    Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

    Lanes 1-3: Merged signal (red and green). Green - ab2783 observed at 140 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.

    ab2783 Anti-Calcium Pump PMCA4 ATPase antibody [JA9] was shown to specifically react with Calcium Pump PMCA4 ATPase in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ATP2B4 (Calcium Pump PMCA4 ATPase) knockout HeLa cell line ab264720 (knockout cell lysate Human ATP2B4 (Calcium Pump PMCA4 ATPase) knockout HeLa cell lysate ab258321) was used. Wild-type and Calcium Pump PMCA4 ATPase knockout samples were subjected to SDS-PAGE. ab2783 and Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783) at 1/500 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ATP2B4 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human ATP2B4 (Calcium Pump PMCA4 ATPase) knockout HeLa cell line (Human ATP2B4 (Calcium Pump PMCA4 ATPase) knockout HeLa cell line ab264720)

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 138 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783), expandable thumbnail

    Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

    Lanes 1-3: Merged signal (red and green). Green - ab2783 observed at 140 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.

    ab2783 Anti-Calcium Pump PMCA4 ATPase antibody [JA9] was shown to specifically react with Calcium Pump PMCA4 ATPase in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ATP2B4 (Calcium Pump PMCA4 ATPase) knockout HeLa cell line ab264720 (knockout cell lysate Human ATP2B4 (Calcium Pump PMCA4 ATPase) knockout HeLa cell lysate ab258321) was used. Wild-type and Calcium Pump PMCA4 ATPase knockout samples were subjected to SDS-PAGE. ab2783 and Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783) at 1/500 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ATP2B4 knockout HeLa cell lysate at 20 µg

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 138 kDa

    Observed band size: 140 kDa

  • Immunocytochemistry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783), expandable thumbnail

    Immunocytochemistry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

    Immunolocalization of Calcium Pump PMCA4 ATPase in human corneal epithelium.

    (A) Immunolocalization of PMCA4 (green) using ab2783 on cell membranes of all cells in each layer of human corneal epithelium.

    (B) Immunolocalization of Calcium Sensing Receptor (red) using Anti-CaSR antibody [5C10, ADD] ab19347 on cell membranes of human corneal epithelium.

    (C) Merged image.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

    IHC image of ab2783 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2783, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783), expandable thumbnail

    Immunocytochemistry - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

    Immunolocalization of Calcium Pump PMCA4 and Calcium Sensing Receptor in bovine corneal epithelium (bCE).

    (A) Immunolocalization of Calcium Pump PMCA4 (green) using ab2783 on cell membranes of all cells in each layer of bCE.

    (B) Immunolocalization of Calcium Sensing Receptor (red) using Anti-CaSR antibody [5C10, ADD] ab19347 on cell membranes of bCE mostly in wing- and squamous cell layers.

    (C) Merged image.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody [JA9] (ab2783)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing PMCA4 ATPase ab2783 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

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