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AB325847

Anti-CALCRL antibody [HL3876] - BSA and Azide free

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Rabbit Recombinant Monoclonal CRLR antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CALCRL.

View Alternative Names

CGRPR, CALCRL, Calcitonin gene-related peptide type 1 receptor, CGRP type 1 receptor, Calcitonin receptor-like receptor, CRLR

3 Images
Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (AB325847)
  • WB

Supplier Data

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (AB325847)

Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325847 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (ab325847) at 1/1000 dilution

Lane 1:

Unboiled HUVEC whole cell extracts at 30 µg

Lane 2:

Unboiled HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (AB325847)
  • WB

Supplier Data

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (AB325847)

Boiled and unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325847 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (ab325847) at 1/1000 dilution

Lane 1:

Boiled HUVEC whole cell extracts at 30 µg

Lane 2:

Boiled HeLa whole cell extracts at 30 µg

Lane 3:

Unboiled HUVEC whole cell extracts at 30 µg

Lane 4:

Unboiled HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (AB325847)
  • WB

Supplier Data

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (AB325847)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325847 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CALCRL antibody [HL3876] - BSA and Azide free (ab325847) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tag CALCRL transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tag CALCRL transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL3876

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human CALCRL.

Q16602

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G protein-coupled receptor which specificity is determined by its interaction with receptor-activity-modifying proteins (RAMPs) (PubMed : 32296767, PubMed : 33602864, PubMed : 8626685). Together with RAMP1, form the receptor complex for calcitonin-gene-related peptides CALCA/CGRP1 and CALCB/CGRP2 (PubMed : 33602864). Together with RAMP2 or RAMP3, function as receptor complexes for adrenomedullin (ADM and ADM2) (PubMed : 32296767, PubMed : 9620797). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway (PubMed : 32296767, PubMed : 8626685).
See full target information CALCRL
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Product promise

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