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AB250894

Anti-calcyphosine antibody [EPR15631] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal calcyphosine antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Calcyphosin, Calcyphosine, CAPS

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-calcyphosine antibody [EPR15631] - BSA and Azide free (AB250894)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-calcyphosine antibody [EPR15631] - BSA and Azide free (AB250894)

This data was developed using ab186740, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling calcyphosine with ab186740 at 1/4000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-calcyphosine antibody [EPR15631] - BSA and Azide free (AB250894)
  • WB

Supplier Data

Western blot - Anti-calcyphosine antibody [EPR15631] - BSA and Azide free (AB250894)

This data was developed using ab186740, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-calcyphosine antibody [EPR15631] (<a href='/en-us/products/primary-antibodies/calcyphosine-antibody-epr15631-ab186740'>ab186740</a>) at 1/1000 dilution

All lanes:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 20 kDa

true

Western blot - Anti-calcyphosine antibody [EPR15631] - BSA and Azide free (AB250894)
  • WB

Supplier Data

Western blot - Anti-calcyphosine antibody [EPR15631] - BSA and Azide free (AB250894)

This data was developed using ab186740, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-calcyphosine antibody [EPR15631] (<a href='/en-us/products/primary-antibodies/calcyphosine-antibody-epr15631-ab186740'>ab186740</a>) at 1/10000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 20 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15631

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250894 is the carrier-free version of ab186740.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcyphosine also known as CAPS is a calcium-binding protein with a molecular mass of approximately 22 kDa. It plays a role in intracellular calcium signaling. Calcyphosine expression occurs widely in multiple tissues with high levels observed in the thyroid gland and epithelial tissues. It binds calcium ions impacting cellular processes such as signal transduction and cell communication.
Biological function summary

Calcyphosine influences several cellular functions related to calcium homeostasis and signaling. It does not form part of any larger complex but modulates cellular activities by regulating calcium ion concentrations inside cells. This regulation is critical for functions like muscle contraction hormone secretion and neurotransmitter release. Its presence in different tissues suggests diverse roles depending on the cellular environment.

Pathways

Calcyphosine plays a role in calcium-dependent pathways significant for maintaining cellular activities. It intersects with the calcium signaling pathway affecting proteins like calmodulin and calcineurin which further mediate various cellular responses. These interactions alter signal transmission processes vital for cell responses and adaptations.

Studies have noted calcyphosine's involvement in thyroid-related conditions and some cancers. Altered expression levels link the protein to thyroid disorders potentially affecting thyroid hormone production and regulation. Calcyphosine's interaction with proteins like TSH receptor and thyroid transcription factors highlights its role in thyroid function and its potential influence on thyroid pathology and neoplastic conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-binding protein. May play a role in cellular signaling events (Potential).
See full target information Calcyphosin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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