Rabbit Polyclonal CalDAG-GEFI antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Predicted |
Cow | Predicted |
Pig | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
CDC25L, MCG7, RASGRP2, RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein, CalDAG-GEFI, hCDC25L
Rabbit Polyclonal CalDAG-GEFI antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
CalDAG-GEFI also known as RasGRP2 is a guanine nucleotide exchange factor that plays a critical role in cellular signaling by activating small GTPases like Rap1. The protein consists of 546 amino acids giving it a molecular mass of 61 kDa. It is widely expressed in platelets leukocytes and the brain. The signaling pathways that involve CalDAG-GEFI are essential for the regulation of calcium (Ca2+) levels within cells impacting many cellular functions.
CalDAG-GEFI influences cell adhesion and migration by activating Rap1 a member of the Ras family of GTPases. This protein does not work alone and often forms part of signaling complexes that help relay external signals to intracellular responses. Through its regulatory effects on Rap1 CalDAG-GEFI affects platelet aggregation and leukocyte trafficking which are important for normal immune function and hemostasis.
CalDAG-GEFI plays significant roles in the Ras and Rap1 signaling pathways. It operates with other proteins like PLC (phospholipase C) to mediate calcium-dependent activation of Rap1. This activity links CalDAG-GEFI to the regulation of integrins affecting cellular interactions with the extracellular matrix. This linkage is critical for various cellular activities including immune surveillance and tissue repair processes.
CalDAG-GEFI relates mainly to bleeding disorders and immune system dysfunctions. Its role in platelet aggregation makes it significant in conditions like Glanzmann thrombasthenia where platelet plugs fail to form properly. Furthermore improper regulation of CalDAG-GEFI can contribute to autoimmune diseases due to dysregulated leukocyte migration. This protein's activity is closely associated with integrins which also play roles in these conditions underlining its importance in maintaining normal hemostatic balance and immune response.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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7.5% SDS PAGE
All lanes: Western blot - Anti-CalDAG-GEFI antibody (ab137608) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 69 kDa
7.5% SDS PAGE
All lanes: Western blot - Anti-CalDAG-GEFI antibody (ab137608) at 1/1000 dilution
All lanes: Mouse liver lysate at 50 µg
Predicted band size: 69 kDa
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