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AB137608

Anti-CalDAG-GEFI antibody

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(4 Publications)

Rabbit Polyclonal CalDAG-GEFI antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus.

View Alternative Names

CDC25L, MCG7, RASGRP2, RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein, CalDAG-GEFI, hCDC25L

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CalDAG-GEFI antibody (AB137608)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CalDAG-GEFI antibody (AB137608)

Immunohistochemical analysis of paraffin-embedded hepatoma tissue stained for RASGRP2 protein at cytosol with ab137608 at a 1/200 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

Immunoprecipitation - Anti-CalDAG-GEFI antibody (AB137608)
  • IP

Supplier Data

Immunoprecipitation - Anti-CalDAG-GEFI antibody (AB137608)

Immunoprecipitation of RASGRP2 protein from Raji whole cell extracts using 5 µg of ab137608. Western blot analysis was performed using ab137608.

All lanes:

Immunoprecipitation - Anti-CalDAG-GEFI antibody (ab137608) at 5 µg

Lane 1:

Raji whole cell extracts with control IgG

Lane 2:

Raji whole cell extracts

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 69 kDa

false

Western blot - Anti-CalDAG-GEFI antibody (AB137608)
  • WB

Supplier Data

Western blot - Anti-CalDAG-GEFI antibody (AB137608)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CalDAG-GEFI antibody (ab137608) at 1/1000 dilution

Lane 1:

Raji whole cell lysates at 30 µg

Lane 2:

NT2D1 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 68 kDa

false

Western blot - Anti-CalDAG-GEFI antibody (AB137608)
  • WB

Unknown

Western blot - Anti-CalDAG-GEFI antibody (AB137608)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CalDAG-GEFI antibody (ab137608) at 1/1000 dilution

All lanes:

Mouse liver lysate at 50 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7LDG7

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Complementary Products

Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB96293

Anti-RASGRP 4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRP 4 antibody (AB96293)

  • 0
  • 0
Primary Antibodies

AB96085

Anti-VBP-1 antibody

Western blot - Anti-VBP-1 antibody (AB96085)

  • 0
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Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CalDAG-GEFI also known as RasGRP2 is a guanine nucleotide exchange factor that plays a critical role in cellular signaling by activating small GTPases like Rap1. The protein consists of 546 amino acids giving it a molecular mass of 61 kDa. It is widely expressed in platelets leukocytes and the brain. The signaling pathways that involve CalDAG-GEFI are essential for the regulation of calcium (Ca2+) levels within cells impacting many cellular functions.
Biological function summary

CalDAG-GEFI influences cell adhesion and migration by activating Rap1 a member of the Ras family of GTPases. This protein does not work alone and often forms part of signaling complexes that help relay external signals to intracellular responses. Through its regulatory effects on Rap1 CalDAG-GEFI affects platelet aggregation and leukocyte trafficking which are important for normal immune function and hemostasis.

Pathways

CalDAG-GEFI plays significant roles in the Ras and Rap1 signaling pathways. It operates with other proteins like PLC (phospholipase C) to mediate calcium-dependent activation of Rap1. This activity links CalDAG-GEFI to the regulation of integrins affecting cellular interactions with the extracellular matrix. This linkage is critical for various cellular activities including immune surveillance and tissue repair processes.

CalDAG-GEFI relates mainly to bleeding disorders and immune system dysfunctions. Its role in platelet aggregation makes it significant in conditions like Glanzmann thrombasthenia where platelet plugs fail to form properly. Furthermore improper regulation of CalDAG-GEFI can contribute to autoimmune diseases due to dysregulated leukocyte migration. This protein's activity is closely associated with integrins which also play roles in these conditions underlining its importance in maintaining normal hemostatic balance and immune response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
See full target information RASGRP2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadn5417 PubMed39576856

2024

CalDAG-GEFI acts as a guanine nucleotide exchange factor for LRRK2 to regulate LRRK2 function and neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Qinfang Liu,Bingxu Huang,Noah Guy Lewis Guiberson,Shifan Chen,Dong Zhu,Gang Ma,Xin-Ming Ma,Jill R Crittenden,Jianzhong Yu,Ann M Graybiel,Ted M Dawson,Valina L Dawson,Yulan Xiong

The Journal of clinical investigation 128:4359-4371 PubMed30024857

2018

JAK2-V617F promotes venous thrombosis through β1/β2 integrin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Bärbel Edelmann,Nibedita Gupta,Tina M Schnoeder,Anja M Oelschlegel,Khurrum Shahzad,Jürgen Goldschmidt,Lars Philipsen,Soenke Weinert,Aniket Ghosh,Felix C Saalfeld,Subbaiah Chary Nimmagadda,Peter Müller,Rüdiger Braun-Dullaeus,Juliane Mohr,Denise Wolleschak,Stefanie Kliche,Holger Amthauer,Florian H Heidel,Burkhart Schraven,Berend Isermann,Andreas J Müller,Thomas Fischer

Scientific reports 8:4244 PubMed29523808

2018

Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kanika Vanshylla,Caren Bartsch,Christoffer Hitzing,Laura Krümpelmann,Jürgen Wienands,Niklas Engels

Nature communications 5:4657 PubMed25118589

2014

RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase.

Applications

WB

Species

Mouse

Songqing Tang,Taoyong Chen,Zhou Yu,Xuhui Zhu,Mingjin Yang,Bin Xie,Nan Li,Xuetao Cao,Jianli Wang
View all publications
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Product promise

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