Skip to main content

Rabbit Polyclonal CalDAG-GEFI antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus.

Be the first to review this product! Submit a review

Images

Western blot - Anti-CalDAG-GEFI antibody (AB137608), expandable thumbnail
  • Western blot - Anti-CalDAG-GEFI antibody (AB137608), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7LDG7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Predicted
Cow
Predicted
Pig
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Target data

Function

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.

Alternative names

Recommended products

Rabbit Polyclonal CalDAG-GEFI antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human RASGRP2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7LDG7
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CalDAG-GEFI also known as RasGRP2 is a guanine nucleotide exchange factor that plays a critical role in cellular signaling by activating small GTPases like Rap1. The protein consists of 546 amino acids giving it a molecular mass of 61 kDa. It is widely expressed in platelets leukocytes and the brain. The signaling pathways that involve CalDAG-GEFI are essential for the regulation of calcium (Ca2+) levels within cells impacting many cellular functions.

Biological function summary

CalDAG-GEFI influences cell adhesion and migration by activating Rap1 a member of the Ras family of GTPases. This protein does not work alone and often forms part of signaling complexes that help relay external signals to intracellular responses. Through its regulatory effects on Rap1 CalDAG-GEFI affects platelet aggregation and leukocyte trafficking which are important for normal immune function and hemostasis.

Pathways

CalDAG-GEFI plays significant roles in the Ras and Rap1 signaling pathways. It operates with other proteins like PLC (phospholipase C) to mediate calcium-dependent activation of Rap1. This activity links CalDAG-GEFI to the regulation of integrins affecting cellular interactions with the extracellular matrix. This linkage is critical for various cellular activities including immune surveillance and tissue repair processes.

Associated diseases and disorders

CalDAG-GEFI relates mainly to bleeding disorders and immune system dysfunctions. Its role in platelet aggregation makes it significant in conditions like Glanzmann thrombasthenia where platelet plugs fail to form properly. Furthermore improper regulation of CalDAG-GEFI can contribute to autoimmune diseases due to dysregulated leukocyte migration. This protein's activity is closely associated with integrins which also play roles in these conditions underlining its importance in maintaining normal hemostatic balance and immune response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-CalDAG-GEFI antibody (ab137608), expandable thumbnail

    Western blot - Anti-CalDAG-GEFI antibody (ab137608)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-CalDAG-GEFI antibody (ab137608) at 1/1000 dilution

    All lanes: Raji whole cell lysate at 30 µg

    Predicted band size: 69 kDa

  • Western blot - Anti-CalDAG-GEFI antibody (ab137608), expandable thumbnail

    Western blot - Anti-CalDAG-GEFI antibody (ab137608)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-CalDAG-GEFI antibody (ab137608) at 1/1000 dilution

    All lanes: Mouse liver lysate at 50 µg

    Predicted band size: 69 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com