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Rabbit Polyclonal Caldesmon/CDM antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CALD1 aa 500-550.

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Images

Immunoprecipitation - Anti-Caldesmon/CDM antibody (AB183146), expandable thumbnail
  • Western blot - Anti-Caldesmon/CDM antibody (AB183146), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human CALD1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q05682

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Chinese hamster
Predicted
Predicted
Common marmoset
Predicted
Predicted
Dog
Predicted
Predicted
Gorilla
Predicted
Predicted
Guinea pig
Predicted
Predicted
Macaque monkey
Predicted
Predicted
Orangutan
Predicted
Predicted
Rhesus monkey
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mg of lysate

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Guinea pig, Dog, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Guinea pig, Dog, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan

Dilution info

-

Notes

-

Associated Products

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Target data

Function

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal Caldesmon/CDM antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CALD1 aa 500-550.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human CALD1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q05682
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab183146 was affinity purified using an epitope specific to Caldesmon/CDM immobilized on solid support.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Caldesmon also known as CDM CALD1 and h-caldesmon is a cytoskeletal protein with a molecular mass of approximately 93 to 120 kDa. This protein expresses itself abundantly in smooth muscle tissues yet it can also be found in non-muscle cells like fibroblasts and endothelial cells. Researchers often use specific caldesmon antibodies in immunohistochemistry allowing them to label various cellular components and providing insights into tissue composition. Due to its presence in muscle tissues caldesmon is essential for understanding muscle physiology and pathology.

Biological function summary

Caldesmon interacts with actin and myosin to regulate actomyosin contractility. This protein plays a critical role in controlling the contraction and relaxation processes in smooth muscle cells. Caldesmon forms part of a complex that includes calmodulin and tropomyosin enhancing its ability to stabilize actin filaments. It functions by inhibiting the ATPase activity of myosin therefore influencing cellular motility and shape change mechanisms. Researchers continually study caldesmon to comprehend its interactome and its significance within the larger cellular structure.

Pathways

Caldesmon participates in the regulation of the cytoskeletal dynamics vital for cell motility and structural integrity. In particular it is an important component of the contraction-relaxation cycle pathway in smooth muscle tissues. This protein has connections with pathways involving RhoA-Rho kinase where caldesmon modulates the phosphorylation levels influencing muscle contraction. Additionally proteins like tropomyosin and calmodulin modulate its activity especially under calcium-calmodulin-dependent pathways which further elucidates its regulatory importance.

Associated diseases and disorders

Caldesmon has associations with conditions like certain types of cancers and cardiovascular diseases. Its expression levels and distribution provide valuable information in identifying smooth muscle tumors and other pathological conditions. In oncology for example h-caldesmon serves as a marker to distinguish leiomyosarcomas from other tumors. Moreover due to its involvement in smooth muscle contractility caldesmon links with proteins such as calmodulin and tropomyosin in diseases where abnormal contraction and cellular motility play significant roles. Understanding these connections is important for developing targeted treatments and improving diagnostic accuracy.

Product promise

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2 product images

  • Immunoprecipitation - Anti-Caldesmon/CDM antibody (ab183146), expandable thumbnail

    Immunoprecipitation - Anti-Caldesmon/CDM antibody (ab183146)

    Immunoprecipitation of HeLa whole cell lysate using ab183146 at 6 μg/mg of lysate (1 mg/IP; 20% of IP loaded per lane) followed by detection of Caldesmon/CDM with ab183146 at 1 μg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.

    Exposure: 10second

    developed using the ECL technique

    All lanes: Immunoprecipitation - Anti-Caldesmon/CDM antibody (ab183146)

    Developed using the ECL technique.

    Predicted band size: 93 kDa

    Exposure time: 10s

  • Western blot - Anti-Caldesmon/CDM antibody (ab183146), expandable thumbnail

    Western blot - Anti-Caldesmon/CDM antibody (ab183146)

    All lanes: Western blot - Anti-Caldesmon/CDM antibody (ab183146) at 0.1 µg/mL

    Lane 1: HeLa whole cell lysate at 50 µg

    Lane 2: 293T whole cell lysate at 50 µg

    Lane 3: Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 93 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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