Rabbit Polyclonal Caldesmon/CDM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Predicted | Predicted | Predicted |
Chimpanzee | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Rabbit | Predicted | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Rabbit, Cow, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Rabbit, Cow, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Rabbit, Cow, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
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Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
CAD, CDM, CALD1, Caldesmon, CDM
Rabbit Polyclonal Caldesmon/CDM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Full details and terms and conditions can be found here:
Terms & Conditions.
The band seen at 78 kDa is consistent with the banding pattern observed for other commercially available antibodies to Caldesmon/CDM.
All lanes: Western blot - Anti-Caldesmon/CDM antibody (ab68878) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: SK N SH (Human neuroblastoma) Whole Cell Lysate at 10 µg
Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 78 kDa
ICC/IF image of ab68878 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68878, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2, Hek293 and MCF7 cells at 5µg/ml.
IHC image of Caldesmon/CDM staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68878 at 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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