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Rabbit Polyclonal Caldesmon/CDM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.

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Images

Western blot - Anti-Caldesmon/CDM antibody (AB68878), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Caldesmon/CDM antibody (AB68878), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody (AB68878), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Rabbit, Cow, Chimpanzee, Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Rat, Rabbit, Cow, Chimpanzee, Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Rabbit, Cow, Chimpanzee, Rhesus monkey

Dilution info

-

Notes

-

Associated Products

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Target data

Function

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal Caldesmon/CDM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-Caldesmon/CDM antibody (ab68878), expandable thumbnail

    Western blot - Anti-Caldesmon/CDM antibody (ab68878)

    The band seen at 78 kDa is consistent with the banding pattern observed for other commercially available antibodies to Caldesmon/CDM.

    All lanes: Western blot - Anti-Caldesmon/CDM antibody (ab68878) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: SK N SH (Human neuroblastoma) Whole Cell Lysate at 10 µg

    Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 93 kDa

    Observed band size: 78 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Caldesmon/CDM antibody (ab68878), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Caldesmon/CDM antibody (ab68878)

    ICC/IF image of ab68878 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68878, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2, Hek293 and MCF7 cells at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody (ab68878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody (ab68878)

    IHC image of Caldesmon/CDM staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68878 at 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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