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AB216011

Anti-Caldesmon/CDM antibody [CALD1/820]

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(1 Publication)

Mouse Monoclonal Caldesmon/CDM antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CALD1.

View Alternative Names

CAD, CDM, CALD1, Caldesmon

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [CALD1/820] (AB216011)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [CALD1/820] (AB216011)

Immunohistochemical analysis of formalin fixed paraffin embedded Human uterus tissue labeling Caldesmon/CDM with ab216011 at 0.5 μg/ml.

Western blot - Anti-Caldesmon/CDM antibody [CALD1/820] (AB216011)
  • WB

Unknown

Western blot - Anti-Caldesmon/CDM antibody [CALD1/820] (AB216011)

All lanes:

Western blot - Anti-Caldesmon/CDM antibody [CALD1/820] (ab216011) at 4 µg/mL

All lanes:

Human ovary tissue

Predicted band size: 93 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CALD1/820

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CALD1.

Q05682

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Alternative Products

Primary Antibodies

AB214629

Alexa Fluor® 555 Anti-Caldesmon/CDM antibody [E89]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Caldesmon/CDM antibody [E89] (AB214629)

  • 0
  • 0
Primary Antibodies

AB32330

Anti-Caldesmon/CDM antibody [E89]

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  • 0
  • 0
Primary Antibodies

AB76106

Anti-Caldesmon/CDM (phospho S759) antibody [EPR2212]

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Western blot - Anti-Caldesmon/CDM (phospho S759) antibody [EPR2212] (AB76106)

  • 0
  • 0
Primary Antibodies

AB183339

Anti-Caldesmon/CDM antibody [SP226]

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  • 0
  • 0
Primary Antibodies

AB215275

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [E89] - BSA and Azide free (AB215275)

  • 0
  • 0
Primary Antibodies

AB238782

Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (AB238782)

  • 0
  • 0

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB8227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "<p>Primary incubation was for 30 minutes at RT.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab216011 was purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caldesmon also known as CDM CALD1 and h-caldesmon is a cytoskeletal protein with a molecular mass of approximately 93 to 120 kDa. This protein expresses itself abundantly in smooth muscle tissues yet it can also be found in non-muscle cells like fibroblasts and endothelial cells. Researchers often use specific caldesmon antibodies in immunohistochemistry allowing them to label various cellular components and providing insights into tissue composition. Due to its presence in muscle tissues caldesmon is essential for understanding muscle physiology and pathology.
Biological function summary

Caldesmon interacts with actin and myosin to regulate actomyosin contractility. This protein plays a critical role in controlling the contraction and relaxation processes in smooth muscle cells. Caldesmon forms part of a complex that includes calmodulin and tropomyosin enhancing its ability to stabilize actin filaments. It functions by inhibiting the ATPase activity of myosin therefore influencing cellular motility and shape change mechanisms. Researchers continually study caldesmon to comprehend its interactome and its significance within the larger cellular structure.

Pathways

Caldesmon participates in the regulation of the cytoskeletal dynamics vital for cell motility and structural integrity. In particular it is an important component of the contraction-relaxation cycle pathway in smooth muscle tissues. This protein has connections with pathways involving RhoA-Rho kinase where caldesmon modulates the phosphorylation levels influencing muscle contraction. Additionally proteins like tropomyosin and calmodulin modulate its activity especially under calcium-calmodulin-dependent pathways which further elucidates its regulatory importance.

Caldesmon has associations with conditions like certain types of cancers and cardiovascular diseases. Its expression levels and distribution provide valuable information in identifying smooth muscle tumors and other pathological conditions. In oncology for example h-caldesmon serves as a marker to distinguish leiomyosarcomas from other tumors. Moreover due to its involvement in smooth muscle contractility caldesmon links with proteins such as calmodulin and tropomyosin in diseases where abnormal contraction and cellular motility play significant roles. Understanding these connections is important for developing targeted treatments and improving diagnostic accuracy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
See full target information CALD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107891 PubMed37766980

2023

Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mick J M van Eijs,José J M Ter Linde,Matthijs J D Baars,Mojtaba Amini,Miangela M Laclé,Eelco C Brand,Eveline M Delemarre,Julia Drylewicz,Stefan Nierkens,Rik J Verheijden,Bas Oldenburg,Yvonne Vercoulen,Karijn P M Suijkerbuijk,Femke van Wijk
View all publications
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Product promise

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