Anti-Caldesmon/CDM antibody [E89]
- BOND RX™ Validated
- RabMAb
- Recombinant
- KO Validated
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(33 Publications)
Rabbit Recombinant Monoclonal Caldesmon/CDM antibody. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 33 publications.
View Alternative Names
CAD, CDM, CALD1, Caldesmon
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human leiomyoma tissue sections labeling Caldesmon/CDM with purified ab32330 at 1/100 dilution (1.21 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling Caldesmon/CDM with purified ab32330 at 1/100 dilution (1.21 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Caldesmon/CDM with purified ab32330 at 1/100 dilution (1.21 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling Caldesmon/CDM with purified ab32330 at 1/50 dilution (2.4 μg/mL). Cells were fixed in 100% Methanol and permeabilized with 0.1% TritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labelling Caldesmon/CDM with purified ab32330 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
- IP
Lab
Immunoprecipitation - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Purified ab32330 at 1/20 dilution (0.6μg) immunoprecipitating Caldesmon/CDM in NIH/3T3 whole cell lysate.
Lane 1 (input) : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate.
Lane 2 (+) : ab32330 + NIH/3T3 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32330 in NIH/3T3 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-Caldesmon/CDM antibody [E89] (ab32330)
Predicted band size: 93 kDa
Observed band size: 70 kDa
false
- WB
Lab
Western blot - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Lanes 1-4 : Merged signal (red and green). Green - ab32330 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab32330 Anti-Caldesmon/CDM antibody [E89] was shown to specifically react with Caldesmon/CDM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265026 (knockout cell lysate ab257375) was used. Wild-type and Caldesmon/CDM knockout samples were subjected to SDS-PAGE. ab32330 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Caldesmon/CDM antibody [E89] (ab32330) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CALD1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CALD1 (Caldesmon/CDM) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cald1-caldesmon-cdm-knockout-hela-cell-line-ab265026'>ab265026</a>)
Lane 3:
NIH/3T3 cell lysate at 20 µg
Lane 4:
HEK-293 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 75 kDa
false
- WB
Unknown
Western blot - Anti-Caldesmon/CDM antibody [E89] (AB32330)
All lanes:
Western blot - Anti-Caldesmon/CDM antibody [E89] (ab32330) at 1/10000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2:
NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 3:
Rat liver lysate at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 93 kDa
false
- WB
CiteAb
Western blot - Anti-Caldesmon/CDM antibody [E89] (AB32330)
Caldesmon/CDM western blot using anti-Caldesmon/CDM antibody [E89] ab32330. Publication image and figure legend from Ferrán, B., Martí-Pàmies, I., et al., 2016, Sci Rep, PubMed 27181368.
ab32330 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32330 please see the product overview.
Lentiviral NOR-1 over-expression induces phenotypic changes in human VSMC and up-regulates SMPX.VSMC were transduced with lentiviral vectors to over-express NOR-1 (pLVX/NOR-1; pNOR-1), a FLAG-tagged derivative form of NOR-1 (pLVX/NOR-1-FLAG; pNOR-1F) or with control lentiviral vectors (pLVX or the EGFP expression vector [pLVX/EGFP; pGFP]). (a) Immunofluorescence microscopy analysis showing FLAG-tagged NOR-1 (green), filamentous actin (F-actin stained with phalloidin, red) and nuclei (Hoechst stain, blue). (b) Expression levels (analyzed by real-time PCR) of a panel of genes related to cell shape/morphology encoding for cytoskeleton and cytoskeletal-associated proteins in control cells (white bars) and cells transduced with pNOR-1F (black bars) : ACTA2 (smooth muscle α-2 actin), MYH10 (myosin heavy chain 10), TPM1 (tropomyosin 1), TAGLN (transgelin), CALD1(1) (caldesmon transcript specific for smooth muscle cells), CALD1(2) (caldesmon transcript ubiquitously expressed, encoding for a light form of the protein [l-CaD]), ACTB (β-actin), PXN (paxillin) and SMPX (small muscle protein, X-linked) (n = 6). *p < 0.0001 vs. cells transduced with pGFP. (c) CALD1(2) and SMPX mRNA levels in VSMC transduced with pNOR-1 or with control lentiviral vectors. Data were normalized as in (b) (n = 6). *p < 0.0001 vs. cells transduced with pLVX. (d) Representative Western blot showing protein levels of NOR-1, SMPX, l-CaD, MYH10 and β-actin in VSMC over-expressing NOR-1 and control cells (n = 4).
false
Related conjugates and formulations (4)
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Caldesmon/CDM antibody [E89]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Caldesmon/CDM antibody [E89]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Caldesmon/CDM antibody [E89]
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Anti-Caldesmon/CDM antibody [E89] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Caldesmon interacts with actin and myosin to regulate actomyosin contractility. This protein plays a critical role in controlling the contraction and relaxation processes in smooth muscle cells. Caldesmon forms part of a complex that includes calmodulin and tropomyosin enhancing its ability to stabilize actin filaments. It functions by inhibiting the ATPase activity of myosin therefore influencing cellular motility and shape change mechanisms. Researchers continually study caldesmon to comprehend its interactome and its significance within the larger cellular structure.
Pathways
Caldesmon participates in the regulation of the cytoskeletal dynamics vital for cell motility and structural integrity. In particular it is an important component of the contraction-relaxation cycle pathway in smooth muscle tissues. This protein has connections with pathways involving RhoA-Rho kinase where caldesmon modulates the phosphorylation levels influencing muscle contraction. Additionally proteins like tropomyosin and calmodulin modulate its activity especially under calcium-calmodulin-dependent pathways which further elucidates its regulatory importance.
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Target data
Publications (33)
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Journal of translational medicine 21:766 PubMed37904179
2023
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International immunopharmacology 115:109702 PubMed37724952
2023
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Frontiers in cell and developmental biology 11:993741 PubMed37077418
2023
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Nature communications 13:6032 PubMed36229430
2022
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Biology of reproduction 107:1540-1550 PubMed36094838
2022
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International journal of molecular sciences 23: PubMed35806460
2022
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Journal of cellular physiology 237:2503-2515 PubMed35224740
2022
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Annals of translational medicine 9:1441 PubMed34733993
2021
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Cancer cell international 21:283 PubMed34051818
2021
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FEBS open bio 11:207-225 PubMed33135334
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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