Rabbit Polyclonal CALHM1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CALHM1.
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- Frontiers in physiology 12:7147852021Upregulation of Calcium Homeostasis Modulators in Contractile-To-Proliferative Phenotypical Transition of Pulmonary Arterial Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisela Rodriguez,Jiyuan Chen,Pritesh P Jain,Aleksandra Babicheva,Mingmei Xiong,Jifeng Li,Ning Lai,Tengteng Zhao,Moises Hernandez,Angela Balistrieri,Sophia Parmisano,Tatum Simonson,Ellen Breen,Daniela Valdez-Jasso,Patricia A Thistlethwaite,John Y-J Shyy,Jian Wang,Joe G N Garcia,Ayako Makino,Jason X-J YuanPubMed 34408668
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CALHM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IU99
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Rat | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 5 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 20 µg/mL | Notes - |
Target data
Function
Pore-forming subunit of gustatory voltage-gated ion channels required for sensory perception of sweet, bitter and umami tastes (By similarity). With CALHM3 forms a fast-activating voltage-gated ATP-release channel in type II taste bud cells, ATP acting as a neurotransmitter to activate afferent neural gustatory pathways (By similarity) (PubMed:23467090). Acts both as a voltage-gated and calcium-activated ion channel: mediates neuronal excitability in response to membrane depolarization and low extracellular Ca(2+) concentration (PubMed:22711817, PubMed:23300080). Has poor ion selectivity and forms a wide pore (around 14 Angstroms) that mediates permeation of small ions including Ca(2+), Na(+), K(+) and Cl(-), as well as larger ions such as ATP(4-) (PubMed:22711817, PubMed:23300080, PubMed:32832629, PubMed:37380652). Mediates Ca(2+) influx and downstream activation of the ERK1 and ERK2 cascade in neurons (PubMed:23345406). Triggers endoplasmic reticulum stress by reducing the Ca(2+) content of the endoplasmic reticulum (PubMed:21574960). May indirectly control amyloid precursor protein (APP) proteolysis and aggregated amyloid-beta (Abeta) peptides levels in a Ca(2+)-dependent manner (PubMed:18585350).
Alternative names
FAM26C, CALHM1, Calcium homeostasis modulator protein 1, Protein FAM26C
Recommended products
Rabbit Polyclonal CALHM1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CALHM1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CALHM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IU99
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is predicted to not crossreact with CALHM2 or CALHM3.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CALHM1 also known as Calcium Homeostasis Modulator 1 functions as a channel involved in calcium regulation across cell membranes. It has a mass of approximately 30 kDa. Researchers find CALHM1 predominantly expressed in the nervous system particularly in neurons of the central nervous system. It plays an important role in mediating Ca2+ influx which is essential for various cellular activities.
- Biological function summary
CALHM1 modulates key signaling pathways by forming a large pore channel complex. This complex allows not only calcium ions but also ATP to pass through facilitating extracellular communication and signal transduction. Through these activities CALHM1 impacts synaptic transmission and neuronal excitability influencing overall neuronal communication.
- Pathways
The modulation of calcium signaling by CALHM1 connects closely to the purinergic signaling pathway and the amyloid precursor protein (APP) metabolism pathway. It exhibits interaction with proteins related to these pathways such as APP itself and P2X receptors which are involved in purinergic signaling. CALHM1 therefore bridges critical cellular processes that govern calcium homeostasis and energy coupling.
- Associated diseases and disorders
Studies associate CALHM1 with neurodegenerative conditions such as Alzheimer's disease. Altered function of CALHM1 can impact APP processing linked to Alzheimer's pathology. CALHM1's interaction with APP suggests that dysregulation of its channel activity could contribute to the pathogenesis of the disease. Understanding the relation between CALHM1 and these proteins may offer insights into potential therapeutic targets for neurological disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-CALHM1 antibody (ab106561)
Lane 1: Western blot - Anti-CALHM1 antibody (ab106561) at 1 µg/mL
Lane 2: Western blot - Anti-CALHM1 antibody (ab106561) at 2 µg/mL
All lanes: Rat liver tissue lysate at 15 µg
Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALHM1 antibody (ab106561)
Immunohistochemical analysis of CALHM1 in paraffin embedded Rat liver tissue, using ab106561 at 5 µg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-CALHM1 antibody (ab106561)
Immunofluorescence of CALHM1 in Rat Liver cells using ab106561 at 20 ug/ml.
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