Rabbit Polyclonal CALHM2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CALHM2 aa 100-200.
Images
Publications
Filter by
- Frontiers in physiology 12:7147852021Upregulation of Calcium Homeostasis Modulators in Contractile-To-Proliferative Phenotypical Transition of Pulmonary Arterial Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisela Rodriguez,Jiyuan Chen,Pritesh P Jain,Aleksandra Babicheva,Mingmei Xiong,Jifeng Li,Ning Lai,Tengteng Zhao,Moises Hernandez,Angela Balistrieri,Sophia Parmisano,Tatum Simonson,Ellen Breen,Daniela Valdez-Jasso,Patricia A Thistlethwaite,John Y-J Shyy,Jian Wang,Joe G N Garcia,Ayako Makino,Jason X-J YuanPubMed 34408668
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CALHM2 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HA72
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
Target data
Function
Pore-forming subunit of Ca(2+) homeostasis modulator channels. Mediates ATP release from astrocytes and ATP-induced Ca(2+) influx in microglia thus regulating neuronal ATP and Ca(2+) homeostasis, synaptic transmission and neuroinflammatory response. May form intercellular gap junctions. The gating mechanism remains unknown.
Alternative names
FAM26B, CALHM2, Calcium homeostasis modulator protein 2, Protein FAM26B
Recommended products
Rabbit Polyclonal CALHM2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CALHM2 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CALHM2 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HA72
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CALHM2 also known as Calcium Homeostasis Modulator 2 functions mechanistically as an ion channel that mediates the permeability of calcium ions across the cell membrane. This protein has an approximate molecular mass of 37 kDa. CALHM2 is widely expressed in various tissues including the brain and heart. It is also part of a family of proteins known for their roles in modulating ion channel activity and maintaining calcium homeostasis within cells.
- Biological function summary
CALHM2 plays a role in the regulation of neuronal excitability and neurotransmitter release. This protein is known to form a complex with other CALHM family members where it influences cellular communication within the nervous system. CALHM2 is critical for maintaining the resting membrane potential and modulating the activity of neurons by impacting the flow of calcium and other ions which affects synaptic function.
- Pathways
CALHM2 participates in the calcium signaling pathway which is essential for various cellular processes. It interacts with other proteins such as CALHM1 a related family member to modulate ion permeability. CALHM2 is also involved in the neuronal signaling pathways where it contributes to synaptic plasticity and signal transduction which are essential for memory and cognitive functions.
- Associated diseases and disorders
CALHM2 has associations with neurological disorders such as Alzheimer's disease as it influences calcium homeostasis and signaling in neurons. Disruptions in its function can lead to altered neuronal activity contributing to neurodegenerative conditions. CALHM2's interaction with proteins like amyloid-beta precursor protein (APP) connects its role to the pathology of Alzheimer's disease indicating a potential target for therapeutic intervention in such conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-CALHM2 antibody (ab121446)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121446 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALHM2 antibody (ab121446)
ab121446, at 1/400 dilution, staining CALHM2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Western blot - Anti-CALHM2 antibody (ab121446)
All lanes: Western blot - Anti-CALHM2 antibody (ab121446) at 1/250 dilution
Lane 1: RT-4 cell lysate.
Lane 2: U-251 MG cell lysate.
Lane 3: Human Plasma
Lane 4: Human liver lysate.
Lane 5: Human tonsil lysate.
Developed using the ECL technique.
Predicted band size: 36 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com