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AB249860

Anti-CALHM3 antibody [EPR12513] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CALHM3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FAM26A, CALHM3, Calcium homeostasis modulator protein 3, Protein A

1 Images
Western blot - Anti-CALHM3 antibody [EPR12513] - BSA and Azide free (AB249860)
  • WB

Supplier Data

Western blot - Anti-CALHM3 antibody [EPR12513] - BSA and Azide free (AB249860)

This data was developed using ab175206, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CALHM3 antibody [EPR12513] (<a href='/en-us/products/primary-antibodies/calhm3-antibody-epr12513-ab175206'>ab175206</a>) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HT-1376 cell lysate at 10 µg

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12513

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249860 is the carrier-free version of ab175206.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CALHM3 also known as calcium homeostasis modulator 3 is a voltage-gated ion channel that plays a critical role in cellular calcium signaling. It has a molecular mass of approximately 47 kDa. This protein forms a channel that facilitates the influx and efflux of calcium ions across the plasma membrane influencing membrane potential and cellular excitability. CALHM3 is predominantly expressed in taste bud cells cochlear cells and neurons. Its function is essential for processes that require ion homeostasis within these cells.
Biological function summary

CALHM3 participates in taste perception by working with other proteins to form a pore complex that allows ATP release. This complex is important for signal transmission in taste receptor cells. The ability of CALHM3 channels to release ATP on taste bud stimulation is important for the communication of taste signals to sensory neurons. Through this interaction CALHM3 significantly influences the sensory response and perception of different tastes.

Pathways

CALHM3 is part of the taste signaling pathway interacting with CALHM1 and other subunits to regulate gustatory signals. This pathway plays an important role in how organisms interpret various taste stimuli. Furthermore CALHM3's involvement in calcium signaling pathways connects it with other proteins such as P2X receptors which also mediate ATP signaling. These interactions demonstrate the protein's integration and contribution to essential sensory processes in the body.

Mutations in CALHM3 have links to taste disorders impacting taste perception and sensitivity known as ageusia or dysgeusia. Additionally its role in calcium signaling suggests potential connections to neurological conditions involving altered communication in neural circuits. Alterations in the CALHM3 structure or function can affect its interaction with CALHM1 impacting physiological processes tied to taste and potential neurodegenerative conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of gustatory voltage-gated ion channels required for sensory perception of sweet, bitter and umami tastes. With CALHM1 forms a fast-activating voltage-gated ATP-release channel in type II taste bud cells, ATP acting as a neurotransmitter to activate afferent neural gustatory pathways. Acts both as a voltage-gated and calcium-activated ion channel : mediates neuronal excitability in response to membrane depolarization and low extracellular Ca(2+) concentration. Has poor ion selectivity and forms a wide pore (around 14 Angstroms) that mediates permeation of small ions including Ca(2+), Na(+), K(+) and Cl(-), as well as larger ions such as ATP(4-).
See full target information CALHM3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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