Rabbit Recombinant Monoclonal CALHM3 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-CALHM3 antibody [EPR12513] - BSA and Azide free (AB249860), expandable thumbnail](https://content.abcam.com/products/images/calhm3-antibody-epr12513-bsa-and-azide-free-ab249860--western-blot-img70905.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CALHM3 antibody [EPR12513] - BSA and Azide free (AB249860)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Pore-forming subunit of gustatory voltage-gated ion channels required for sensory perception of sweet, bitter and umami tastes. With CALHM1 forms a fast-activating voltage-gated ATP-release channel in type II taste bud cells, ATP acting as a neurotransmitter to activate afferent neural gustatory pathways. Acts both as a voltage-gated and calcium-activated ion channel: mediates neuronal excitability in response to membrane depolarization and low extracellular Ca(2+) concentration. Has poor ion selectivity and forms a wide pore (around 14 Angstroms) that mediates permeation of small ions including Ca(2+), Na(+), K(+) and Cl(-), as well as larger ions such as ATP(4-).
Alternative names
FAM26A, CALHM3, Calcium homeostasis modulator protein 3, Protein A
Recommended products
Rabbit Recombinant Monoclonal CALHM3 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12513
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249860 is the carrier-free version of Anti-CALHM3 antibody [EPR12513] ab175206.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CALHM3 also known as calcium homeostasis modulator 3 is a voltage-gated ion channel that plays a critical role in cellular calcium signaling. It has a molecular mass of approximately 47 kDa. This protein forms a channel that facilitates the influx and efflux of calcium ions across the plasma membrane influencing membrane potential and cellular excitability. CALHM3 is predominantly expressed in taste bud cells cochlear cells and neurons. Its function is essential for processes that require ion homeostasis within these cells.
- Biological function summary
CALHM3 participates in taste perception by working with other proteins to form a pore complex that allows ATP release. This complex is important for signal transmission in taste receptor cells. The ability of CALHM3 channels to release ATP on taste bud stimulation is important for the communication of taste signals to sensory neurons. Through this interaction CALHM3 significantly influences the sensory response and perception of different tastes.
- Pathways
CALHM3 is part of the taste signaling pathway interacting with CALHM1 and other subunits to regulate gustatory signals. This pathway plays an important role in how organisms interpret various taste stimuli. Furthermore CALHM3's involvement in calcium signaling pathways connects it with other proteins such as P2X receptors which also mediate ATP signaling. These interactions demonstrate the protein's integration and contribution to essential sensory processes in the body.
- Associated diseases and disorders
Mutations in CALHM3 have links to taste disorders impacting taste perception and sensitivity known as ageusia or dysgeusia. Additionally its role in calcium signaling suggests potential connections to neurological conditions involving altered communication in neural circuits. Alterations in the CALHM3 structure or function can affect its interaction with CALHM1 impacting physiological processes tied to taste and potential neurodegenerative conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-CALHM3 antibody [EPR12513] - BSA and Azide free (ab249860), expandable thumbnail](https://content.abcam.com/products/images/calhm3-antibody-epr12513-bsa-and-azide-free-ab249860--western-blot-img70905.jpg "Western blot - Anti-CALHM3 antibody [EPR12513] - BSA and Azide free (ab249860)")
Western blot - Anti-CALHM3 antibody [EPR12513] - BSA and Azide free (ab249860)
This data was developed using Anti-CALHM3 antibody [EPR12513] ab175206, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CALHM3 antibody [EPR12513] (Anti-CALHM3 antibody [EPR12513] ab175206) at 1/1000 dilution
Lane 1: A673 cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HT-1376 cell lysate at 10 µg
Predicted band size: 39 kDa
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