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AB240578

Anti-Calmegin antibody

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(1 Publication)

Rabbit Polyclonal Calmegin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CLGN aa 1-50.

View Alternative Names

Calmegin, CLGN

2 Images
Immunoprecipitation - Anti-Calmegin antibody (AB240578)
  • IP

Supplier Data

Immunoprecipitation - Anti-Calmegin antibody (AB240578)

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Lane 1 : ab240578 used for IP at 8 μl per reaction. For WB 1/1000 dilution.
Lane 2 : Control IgG.
Chemiluminescence detection : 75 seconds.

All lanes:

Immunoprecipitation - Anti-Calmegin antibody (ab240578)

Predicted band size: 61 kDa,70 kDa

false

Western blot - Anti-Calmegin antibody (AB240578)
  • WB

Supplier Data

Western blot - Anti-Calmegin antibody (AB240578)

All lanes:

Western blot - Anti-Calmegin antibody (ab240578) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 70 kDa

false

Exposure time: 75s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CLGN aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O14967

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calmegin also known as CLGN is a protein involved in chaperone activity during spermatogenesis. It has a mass of approximately 65 kDa and is predominantly expressed in the testis. More specifically Calmegin is found in germ cells where it plays a role in the development and maturation of sperm. The protein ensures proper protein folding and stabilization of other proteins during sperm development.
Biological function summary

Calmegin acts as part of a complex necessary for sperm fertility and function. This protein is critical in processing and assembling the molecules required for spermatogenesis. By interacting with other chaperones Calmegin helps to regulate the endoplasmic reticulum's function in germ cells ensuring that proteins reach their target destinations correctly and are folded properly. When functioning as intended these processes facilitate normal sperm development.

Pathways

Calmegin operates within pathways critical to reproductive biology. It is closely tied to the unfolded protein response pathway which maintains protein homeostasis in cells. Additionally it works in concert with proteins like Calnexin in processes that manage protein maturation in the endoplasmic reticulum. All these interactions highlight Calmegin's role in effective protein management during germ cell development affecting male fertility.

Improper function of Calmegin can link to male infertility. Deficiencies or mutations in Calmegin can interfere with spermatogenesis leading to decreased sperm quality or quantity. The protein is also associated with Calreticulin in regulatory functions and abnormalities in these proteins can further impact reproductive health negatively making them critical targets for studies related to infertility treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).
See full target information CLGN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed39450567

2024

S100A16 stabilizes the ITGA3‑mediated ECM‑receptor interaction pathway to drive the malignant properties of lung adenocarcinoma cells via binding MOV10.

Applications

Unspecified application

Species

Unspecified reactive species

Lianren Yang,Ajuan Shen,Rujun Wang,Zhihui Zheng
View all publications
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Product promise

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