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Rabbit Recombinant Monoclonal Calmegin antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Common marmoset, Mouse, Rat samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (AB172477), expandable thumbnail
  • Western blot - Anti-Calmegin antibody [EPR11832-11] (AB172477), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (AB172477), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (AB172477), expandable thumbnail
  • Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (AB172477), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected
Chimpanzee
Predicted
Predicted
Predicted
Predicted
Predicted
Common marmoset
Tested
Expected
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Common marmoset
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Common marmoset, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Species
Rat
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Common marmoset
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Common marmoset, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Common marmoset, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).

Alternative names

Calmegin, CLGN

Recommended products

Rabbit Recombinant Monoclonal Calmegin antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Common marmoset, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11832-11
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Calmegin also known as CLGN is a protein involved in chaperone activity during spermatogenesis. It has a mass of approximately 65 kDa and is predominantly expressed in the testis. More specifically Calmegin is found in germ cells where it plays a role in the development and maturation of sperm. The protein ensures proper protein folding and stabilization of other proteins during sperm development.

Biological function summary

Calmegin acts as part of a complex necessary for sperm fertility and function. This protein is critical in processing and assembling the molecules required for spermatogenesis. By interacting with other chaperones Calmegin helps to regulate the endoplasmic reticulum's function in germ cells ensuring that proteins reach their target destinations correctly and are folded properly. When functioning as intended these processes facilitate normal sperm development.

Pathways

Calmegin operates within pathways critical to reproductive biology. It is closely tied to the unfolded protein response pathway which maintains protein homeostasis in cells. Additionally it works in concert with proteins like Calnexin in processes that manage protein maturation in the endoplasmic reticulum. All these interactions highlight Calmegin's role in effective protein management during germ cell development affecting male fertility.

Associated diseases and disorders

Improper function of Calmegin can link to male infertility. Deficiencies or mutations in Calmegin can interfere with spermatogenesis leading to decreased sperm quality or quantity. The protein is also associated with Calreticulin in regulatory functions and abnormalities in these proteins can further impact reproductive health negatively making them critical targets for studies related to infertility treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477), expandable thumbnail
    This image is courtesy of a customer review from Zachary Yu-Ching Lin

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

  • Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477), expandable thumbnail

    Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    All lanes: Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution

    Lane 1: Human testis tissue lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: Mouse testis tissue lysate at 10 µg

    Lane 4: Rat testis tissue lysate at 10 µg

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (ab172477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.

  • Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477), expandable thumbnail

    Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.

    All lanes: Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Predicted band size: 70 kDa

  • Flow Cytometry (Intracellular) - Anti-Calmegin antibody [EPR11832-11] (ab172477), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Intracellular flow cytometric analysis of permeabilized Jurkat cellslabeling Calmegin with ab172477at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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