AB249695

Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Calmegin antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Common marmoset, Mouse, Rat samples.

View Alternative Names

Calmegin, CLGN

5 Images

Flow Cytometry (Intracellular) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

This data was developed using ab172477, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

This data was developed using ab172477, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

This data was developed using ab172477, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

This data was developed using ab172477, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (<a href='/en-us/products/primary-antibodies/calmegin-antibody-epr11832-11-ab172477'>ab172477</a>)

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

This data was developed using ab172477, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calmegin antibody [EPR11832-11] (<a href='/en-us/products/primary-antibodies/calmegin-antibody-epr11832-11-ab172477'>ab172477</a>) at 1/10000 dilution

Lane 1:

Human testis tissue lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Mouse testis tissue lysate at 10 µg

Lane 4:

Rat testis tissue lysate at 10 µg

Predicted band size: 70 kDa

false

Unconjugated

Anti-Calmegin antibody [EPR11832-11]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11832-11

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Common marmoset

Applications

IP, WB, Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249695 is the carrier-free version of ab172477.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calmegin also known as CLGN is a protein involved in chaperone activity during spermatogenesis. It has a mass of approximately 65 kDa and is predominantly expressed in the testis. More specifically Calmegin is found in germ cells where it plays a role in the development and maturation of sperm. The protein ensures proper protein folding and stabilization of other proteins during sperm development.

Biological function summary

Calmegin acts as part of a complex necessary for sperm fertility and function. This protein is critical in processing and assembling the molecules required for spermatogenesis. By interacting with other chaperones Calmegin helps to regulate the endoplasmic reticulum's function in germ cells ensuring that proteins reach their target destinations correctly and are folded properly. When functioning as intended these processes facilitate normal sperm development.

Pathways

Calmegin operates within pathways critical to reproductive biology. It is closely tied to the unfolded protein response pathway which maintains protein homeostasis in cells. Additionally it works in concert with proteins like Calnexin in processes that manage protein maturation in the endoplasmic reticulum. All these interactions highlight Calmegin's role in effective protein management during germ cell development affecting male fertility.

Improper function of Calmegin can link to male infertility. Deficiencies or mutations in Calmegin can interfere with spermatogenesis leading to decreased sperm quality or quantity. The protein is also associated with Calreticulin in regulatory functions and abnormalities in these proteins can further impact reproductive health negatively making them critical targets for studies related to infertility treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).

See full target information CLGN

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

Western blot - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (AB249695)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Biochemicals

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise