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AB276058

Anti-CALML5 antibody [158] - BSA and Azide free

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Rabbit Recombinant Monoclonal CALML5 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CALML5 aa 1 to C-terminus.

View Alternative Names

CLSP, CALML5, Calmodulin-like protein 5, Calmodulin-like skin protein

2 Images
Flow Cytometry (Intracellular) - Anti-CALML5 antibody [158] - BSA and Azide free (AB276058)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CALML5 antibody [158] - BSA and Azide free (AB276058)

Intracellular flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cells labeling CALML5 with ab276058, followed byFITC-conjugated secondary antibody (black line) compared with a negative control (grey line). The cells were treated according to manufacturer's manual. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-CALML5 antibody [158] - BSA and Azide free (AB276058)
  • ICC

Unknown

Immunocytochemistry - Anti-CALML5 antibody [158] - BSA and Azide free (AB276058)

Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed A431 (human epidermoid carcinoma cell line) cells labeling CALML5 with ab276058 at 1/60 dilution, at 4°C overnight, followed by Goat Anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibody (green) and counterstained with DAPI (blue). Cells were blocked with 10% serum prior incubation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

158

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human CALML5 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZT1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CALML5 also known as calmodulin-like protein 5 is a small calcium-binding protein with a molecular weight of approximately 15 kDa. It shares structural similarities with calmodulin but has a distinct expression pattern. This protein is strongly expressed in keratinocytes particularly in the epidermis and is involved in the calcium-mediated signaling processes that regulate skin cell function and differentiation.
Biological function summary

CALML5 modulates processes involved in epidermal homeostasis and repair. It plays an important role in the regulation of keratinocyte differentiation an essential process for proper skin barrier function. CALML5 does not form part of a large molecular complex but interacts with other calcium-binding proteins to influence epidermal physiology.

Pathways

CALML5 is integrated into the calcium signaling pathway which is critical for calcium-dependent regulation of keratinocyte activity. It relates closely to other proteins within this pathway such as calcium-binding proteins like calmodulin. Its activity is important in relaying calcium signals that influence cellular processes like cell proliferation and differentiation.

CALML5 has links to skin conditions such as psoriasis and certain types of dermatitis where epidermal differentiation is disrupted. CALML5 may interact with proteins such as keratin to influence these disease processes highlighting its potential role in the maintenance of epidermal health and integrity. Understanding its functions could lead to new therapeutic strategies for managing skin-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds calcium. May be involved in terminal differentiation of keratinocytes.
See full target information CALML5
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Product promise

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