Rabbit Recombinant Monoclonal CALM3 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), IP, WB, IHC-Fr and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100467.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793)")
![Immunoprecipitation - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--immunoprecipitation-img6002.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92363.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793)")
![Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--flow-cytometry-intracellular-img56728.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793)")
![Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--flow-cytometry-intracellular-img413923.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (AB214793)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | IP | WB | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected | Tested | Predicted |
| Rat | Expected | Expected | Expected | Tested | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Mouse and rat species reactivity is recommended based on WB results, we do not guarantee IHC for mouse or rat. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Calmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding (PubMed:16760425, PubMed:31454269). Calcium-binding is required for the activation of calmodulin (PubMed:16760425, PubMed:31454269, PubMed:35568036). Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases, such as myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), and phosphatases (PubMed:16760425, PubMed:35568036). Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425).(Microbial infection) Required for C.violaceum CopC and S.flexneri OspC3 arginine ADP-riboxanase activity.
Alternative names
CALML2, CAM3, CAMC, CAMIII, CALM3, CAMIII, CAMC, CAM3, CALML2, Calmodulin-3
Recommended products
Rabbit Recombinant Monoclonal CALM3 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), IP, WB, IHC-Fr and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP799Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab214793 is the carrier-free version of Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calmodulin also known as calmoduline is a multifunctional intermediate calcium-binding protein expressed widely in eukaryotic cells. This small protein has a molecular weight of approximately 16.7 kDa. Calmodulin 1 2 and 3 serve as the key isoforms facilitating a variety of calcium-mediated processes. You can find it in cellular contexts where calcium signaling plays a regulatory role. Due to its ubiquity it acts as a mediator in numerous cellular responses.
- Biological function summary
Calmodulin modulates intracellular activities by binding to calcium ions and interacting with a wide array of target proteins. It is not part of a molecular complex but can partner with proteins like kinases and phosphatases leading to downstream effects on diverse cellular processes. Calmodulin function extends to muscle contraction cell division and signal transduction impacting both basal and specialized cellular functions.
- Pathways
Calmodulin operates as a functional link in calcium signaling and the MAPK signaling pathways. In these pathways calmodulin influences the activity of proteins such as myosin light-chain kinase and calmodulin-dependent protein kinase. These interactions showcase calmodulin's pivotal role in translating calcium signaling into cellular responses connecting several signaling cascades.
- Associated diseases and disorders
Calmodulin has associations with certain cardiovascular diseases and neurodegenerative conditions. Its relationship with ion channel dysfunction links it to disorders like long QT syndrome. Additionally calmodulin interacts with proteins like sodium-calcium exchangers or other ion-handling channels reflecting its involvement in maintaining cellular ionic balance and consequently its implication in pathological states when dysregulated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100467.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)
Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689 staining Calmodulin in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control: PBS in place of primary antibody (inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689).
![Immunoprecipitation - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--immunoprecipitation-img6002.jpg "Immunoprecipitation - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)")
Immunoprecipitation - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)
Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689 immunoprecipitating Calmodulin. 10 μg of cell lysate was incubated with primary antibody at a dilution of 1/20. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: Human skeletal muscle lysate (10 μg)
Lane 2: Human skeletal muscle lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689 in human skeletal muscle lysateThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689).
All lanes: Immunoprecipitation - Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal (Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689)
Observed band size: 16 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92363.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)
Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689 at a 1:250 dilution staining Calmodulin in human urinary bladder carcinoma tissue by immunohistochemistry in paraffin embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689).
![Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--flow-cytometry-intracellular-img56728.jpg "Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)")
Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)
Overlay histogram showing MCF7 (Human breast adenocarcinoma cell line) cells stained with Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689 (red line).
The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C.
Isotype control antibody (black line) was rabbit monoclonal IgG (0.5μg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a decreased signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689).
![Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-ep799y-bsa-and-azide-free-ab214793--flow-cytometry-intracellular-img413923.jpg "Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)")
Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - BSA and Azide free (ab214793)
Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689 staining Calmodulin in NIH/3T3 (Mouse embryo fibroblast cell line) cells by intracellular flow cytometry.
Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabeled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal ab45689).
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