Rabbit Recombinant Monoclonal CALM1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Images
![Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (AB124742), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-epr5028-ab124742--flow-cytometry-intracellular-img114780.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (AB124742)")
![Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (AB124742), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-epr5028-ab124742--western-blot-img111686.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (AB124742)")
Publications
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- Journal of leukocyte biology 111:1021-10292021Flunarizine suppresses Mycobacterium tuberculosis growth via calmodulin-dependent phagosome maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiwei Mo,Xiaoqian Liu,Kehong Zhang,Wenfei Wang,Yi Cai,Qi Ouyang,Chuanzhi Zhu,Dachuan Lin,Haoqiang Wan,Dechang Li,Zhihua Wen,Xinchun ChenPubMed 34533236
- Biochimica et biophysica acta. Molecular cell research 1868:1191192021The heart arrhythmia-linked D130G calmodulin mutation causes premature inhibitory autophosphorylation of CaMKII.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin W Berchtold,Mads Munk,Katarzyna Kulej,Isabel Porth,Lasse Lorentzen,Svetlana Panina,Triantafyllos Zacharias,Martin R Larsen,Jonas M la CourPubMed 34391760
- Circulation research 128:e63-e832020Ca-CaM Dependent Inactivation of RyR2 Underlies Ca Alternans in Intact Heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhong Wei,Jinjing Yao,Darrell Belke,Wenting Guo,Xiaowei Zhong,Bo Sun,Ruiwu Wang,John Paul Estillore,Alexander Vallmitjana,Raul Benitez,Leif Hove-Madsen,Enrique Alvarez-Lacalle,Blas Echebarria,S R Wayne ChenPubMed 33375811
- The FEBS journal 287:2256-22802019Role of cardiac ryanodine receptor calmodulin-binding domains in mediating the action of arrhythmogenic calmodulin N-domain mutation N54I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMads T Søndergaard,Yingjie Liu,Wenting Guo,Jinhong Wei,Ruiwu Wang,Malene Brohus,Michael T Overgaard,S R Wayne ChenPubMed 31763755
- The FEBS journal 286:4554-45782019Diminished inhibition and facilitated activation of RyR2-mediated Ca release is a common defect of arrhythmogenic calmodulin mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMads T Søndergaard,Yingjie Liu,Malene Brohus,Wenting Guo,Alma Nani,Catherine Carvajal,Michael Fill,Michael T Overgaard,S R Wayne ChenPubMed 31230402
- Behavioural brain research 353:32-392018Mechanism of nitric oxide and acid-sensing ion channel 1a modulation of panic-like behaviour in the dorsal periaqueductal grey of the mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Zhou et. al.PubMed 29953907
- Nature communications 9:15042018Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP.
- The Journal of biological chemistry 292:16709-167212017The unique C terminus of the calcineurin isoform CNAβ1 confers non-canonical regulation of enzyme activity by Ca and calmodulin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel Bond,Nina Ly,Martha S CyertPubMed 28842480
- Pflugers Archiv : European journal of physiology 466:1793-8032013A novel calmodulin site in the Cav1.2 N-terminus regulates calcium-dependent inactivation.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Expected | Not recommended | Not recommended |
| Rat | Expected | Expected | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50000 - 1/200000 | Notes - |
Species Mouse | Dilution info 1/50000 - 1/200000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50000 - 1/200000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1/100 - 1/1000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Is a regulator of voltage-dependent L-type calcium channels (PubMed:31454269). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696). Forms a potassium channel complex with KCNQ1 and regulates electrophysiological activity of the channel via calcium-binding (PubMed:25441029). Acts as a sensor to modulate the endomplasmic reticulum contacts with other organelles mediated by VMP1:ATP2A2 (PubMed:28890335).(Microbial infection) Required for Legionella pneumophila SidJ glutamylase activity.
Targets
CALM2, CALM3
Alternative names
Calmodulin-1, CAM1, CAM, CALM, CALM1
Recommended products
Rabbit Recombinant Monoclonal CALM1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR5028
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calmodulin also known as calmoduline is a multifunctional intermediate calcium-binding protein expressed widely in eukaryotic cells. This small protein has a molecular weight of approximately 16.7 kDa. Calmodulin 1 2 and 3 serve as the key isoforms facilitating a variety of calcium-mediated processes. You can find it in cellular contexts where calcium signaling plays a regulatory role. Due to its ubiquity it acts as a mediator in numerous cellular responses.
- Biological function summary
Calmodulin modulates intracellular activities by binding to calcium ions and interacting with a wide array of target proteins. It is not part of a molecular complex but can partner with proteins like kinases and phosphatases leading to downstream effects on diverse cellular processes. Calmodulin function extends to muscle contraction cell division and signal transduction impacting both basal and specialized cellular functions.
- Pathways
Calmodulin operates as a functional link in calcium signaling and the MAPK signaling pathways. In these pathways calmodulin influences the activity of proteins such as myosin light-chain kinase and calmodulin-dependent protein kinase. These interactions showcase calmodulin's pivotal role in translating calcium signaling into cellular responses connecting several signaling cascades.
- Associated diseases and disorders
Calmodulin has associations with certain cardiovascular diseases and neurodegenerative conditions. Its relationship with ion channel dysfunction links it to disorders like long QT syndrome. Additionally calmodulin interacts with proteins like sodium-calcium exchangers or other ion-handling channels reflecting its involvement in maintaining cellular ionic balance and consequently its implication in pathological states when dysregulated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-epr5028-ab124742--flow-cytometry-intracellular-img114780.jpg "Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)")
Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)
Overlay histogram showing SHSY-5Y cells stained with ab124742 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124742, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742), expandable thumbnail](https://content.abcam.com/products/images/calmodulin-1-2-3-antibody-epr5028-ab124742--western-blot-img111686.jpg "Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)")
Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)
All lanes: Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742) at 1/50000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Ramos cell lysate at 10 µg
Lane 3: HCT116 cell lysate at 10 µg
Lane 4: NIH 3T3 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
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