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AB247990

Anti-Calmodulin 1/2/3 antibody [EPR5028] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CALM1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CALM, CAM, CAM1, CALM1, Calmodulin-1

2 Images
Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] - BSA and Azide free (AB247990)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] - BSA and Azide free (AB247990)

This data was developed using ab124742, the same antibody clone in a different buffer formulation.Overlay histogram showing SHSY-5Y cells stained with ab124742 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124742, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] - BSA and Azide free (AB247990)
  • WB

Unknown

Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] - BSA and Azide free (AB247990)

This data was developed using ab124742, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (<a href='/en-us/products/primary-antibodies/calmodulin-1-2-3-antibody-epr5028-ab124742'>ab124742</a>) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

HCT116 cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5028

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab247990 is the carrier-free version of ab124742.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calmodulin also known as calmoduline is a multifunctional intermediate calcium-binding protein expressed widely in eukaryotic cells. This small protein has a molecular weight of approximately 16.7 kDa. Calmodulin 1 2 and 3 serve as the key isoforms facilitating a variety of calcium-mediated processes. You can find it in cellular contexts where calcium signaling plays a regulatory role. Due to its ubiquity it acts as a mediator in numerous cellular responses.
Biological function summary

Calmodulin modulates intracellular activities by binding to calcium ions and interacting with a wide array of target proteins. It is not part of a molecular complex but can partner with proteins like kinases and phosphatases leading to downstream effects on diverse cellular processes. Calmodulin function extends to muscle contraction cell division and signal transduction impacting both basal and specialized cellular functions.

Pathways

Calmodulin operates as a functional link in calcium signaling and the MAPK signaling pathways. In these pathways calmodulin influences the activity of proteins such as myosin light-chain kinase and calmodulin-dependent protein kinase. These interactions showcase calmodulin's pivotal role in translating calcium signaling into cellular responses connecting several signaling cascades.

Calmodulin has associations with certain cardiovascular diseases and neurodegenerative conditions. Its relationship with ion channel dysfunction links it to disorders like long QT syndrome. Additionally calmodulin interacts with proteins like sodium-calcium exchangers or other ion-handling channels reflecting its involvement in maintaining cellular ionic balance and consequently its implication in pathological states when dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Calcium-binding is required for the activation of calmodulin (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases, such as myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), and phosphatases (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed : 16760425). Is a regulator of voltage-dependent L-type calcium channels (PubMed : 31454269). Mediates calcium-dependent inactivation of CACNA1C (PubMed : 26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed : 27165696). Forms a potassium channel complex with KCNQ1 and regulates electrophysiological activity of the channel via calcium-binding (PubMed : 25441029). Acts as a sensor to modulate the endoplasmic reticulum contacts with other organelles mediated by VMP1 : ATP2A2 (PubMed : 28890335).. (Microbial infection) Required for Legionella pneumophila SidJ glutamylase activity.. (Microbial infection) Required for C.violaceum CopC and S.flexneri OspC3 arginine ADP-riboxanase activity.
See full target information CALM1

Additional targets

,CALM3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 77:100019 PubMed35397366

2022

IRF4 suppresses osteogenic differentiation of BM-MSCs by transcriptionally activating miR-636/DOCK9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuepu Zhang,Yue Zhang,Limin Yang,Yuexin Wu,Xiaohu Ma,Gang Tong,Zhaoliang Ban,Haosen Zhao
View all publications
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