Rabbit Polyclonal Calnexin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CANX.
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- Medicine 102:e365822023Bone Marrow Stromal Cells inhibited the growth and metastasis of human U87 cells through delivering exosomal miR-506.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiexiang Zhang,Yu Ding,Wei Zhou,Xiaohong Xu,Jing ZhengPubMed 38065867
- Regenerative therapy 25:35-482023Epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs attenuates spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinghui Xu,Zhenxiao Ren,Tianzuo Niu,Siyuan LiPubMed 38058606
- Biomaterials research 27:1242023Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHochung Jang,Hyosuk Kim,Eun Hye Kim,Geonhee Han,Yeongji Jang,Yelee Kim,Jong Won Lee,Sang Chul Shin,Eunice EunKyeong Kim,Sun Hwa Kim,Yoosoo YangPubMed 38031117
- ACS nano 17:7562-75752023Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Hu,Shu Zhang,Yue Yang,Juan Li,Hongxin Kang,Wenfu Tang,Christopher J Lyon,Meihua WanPubMed 37022097
- Nutrients 15:2023Oral Administration of Bovine Milk-Derived Extracellular Vesicles Attenuates Cartilage Degeneration via Modulating Gut Microbiota in DMM-Induced Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiqi Liu,Haining Hao,Jiankun Li,Ting Zheng,Yukun Yao,Xiaoying Tian,Zhe Zhang,Huaxi YiPubMed 36771453
- Theranostics 11:8570-85862021Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingjun Tong,Haining Hao,Zhe Zhang,Youyou Lv,Xi Liang,Qiqi Liu,Tongjie Liu,Pimin Gong,Lanwei Zhang,Fangfang Cao,Giorgia Pastorin,Chuen Neng Lee,Xiaoyuan Chen,Jiong-Wei Wang,Huaxi YiPubMed 34373759
- Frontiers in neuroscience 14:5482782020A Panel of Exosome-Derived miRNAs of Cerebrospinal Fluid for the Diagnosis of Moyamoya Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Wang et. al.PubMed 33100957
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CANX. The exact immunogen used to generate this antibody is proprietary information. Database link P27824
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Alternative names
Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX
Recommended products
Rabbit Polyclonal Calnexin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CANX.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CANX. The exact immunogen used to generate this antibody is proprietary information. Database link P27824
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.
- Biological function summary
Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.
- Pathways
Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.
- Associated diseases and disorders
Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Calnexin antibody (ab227310)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Calnexin antibody (ab227310) at 1/10000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg
Lanes 2 - 3: Calnexin shRNA transfected HEK-293T whole cell extract at 15 µg
Predicted band size: 68 kDa
Western blot - Anti-Calnexin antibody (ab227310)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Calnexin antibody (ab227310) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell extract at 30 µg
Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab227310)
Paraffin-embedded SW480 (human) xenograft tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Calnexin antibody (ab227310)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Calnexin antibody (ab227310) at 1/1000 dilution
All lanes: Mouse liver lysate at 50 µg
Predicted band size: 68 kDa
Western blot - Anti-Calnexin antibody (ab227310)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Calnexin antibody (ab227310) at 1/10000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 68 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody (ab227310)
Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calnexin (green) using ab227310 at 1/200 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab227310)
Paraffin-embedded human breast carcinoma tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Calnexin antibody (ab227310)
Calnexin western blot using anti-Calnexin antibody ab227310. Publication image and figure legend from Yan, W., Yang, H., et al., 2024, BMC Cancer, PubMed 39039505.
ab227310 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab227310 please see the product overview.
Characterization of hBMSCs-derived exosomes. A The morphology of hBMSCs by inverted microscope. magnification × 400. B The morphology of hBMSCs-Exo by a TEM. scale bar = 200 nm. C Western blot analysis of Exo-surface markers in hBMSCs-Exo and hBMSCs
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