AB227310

Anti-Calnexin antibody

Name: Anti-Calnexin antibody (ab227310) | Abcam
Brand: Abcam Limited
SKU: AB227310
Price: 525 USD
Availability: InStock

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(17 Publications)

Rabbit Polyclonal Calnexin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CANX.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB227310)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB227310)

Paraffin-embedded human breast carcinoma tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB227310)

Paraffin-embedded SW480 (human) xenograft tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody (AB227310)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody (AB227310)

Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calnexin (green) using ab227310 at 1/200 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-Calnexin antibody (AB227310)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Calnexin antibody (ab227310) at 1/10000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg

Lanes 2 - 3:

Calnexin shRNA transfected HEK-293T whole cell extract at 15 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-Calnexin antibody (AB227310)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Calnexin antibody (ab227310) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-Calnexin antibody (AB227310)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Calnexin antibody (ab227310) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell extract at 30 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-Calnexin antibody (AB227310)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Calnexin antibody (ab227310) at 1/1000 dilution

All lanes:

Mouse liver lysate at 50 µg

Predicted band size: 68 kDa

false

CiteAb

Western blot - Anti-Calnexin antibody (AB227310)

Calnexin western blot using anti-Calnexin antibody ab227310. Publication image and figure legend from Yan, W., Yang, H., et al., 2024, BMC Cancer, PubMed 39039505.

ab227310 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab227310 please see the product overview.

Characterization of hBMSCs-derived exosomes. A The morphology of hBMSCs by inverted microscope. magnification × 400. B The morphology of hBMSCs-Exo by a TEM. scale bar = 200 nm. C Western blot analysis of Exo-surface markers in hBMSCs-Exo and hBMSCs

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CANX. The exact immunogen used to generate this antibody is proprietary information.

P27824

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 23:214 PubMed40660178

2025

Organic cation transporter 3 on neuronal mitochondria mediates MPP-induced mitochondrial dysfunction and neurotoxicity in a TIMM22-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ao Guan,Sida Han,Suzhen Liang,Weiwei Shen,Min Guo,Mei Cui

Frontiers in immunology 16:1586174 PubMed40519909

2025

Small extracellular vesicles from human umbilical cord mesenchymal stem cells delivering miR-202-5p alleviate renal ischemia-reperfusion injury by targeting the GOLIM4/PI3K/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Peng,Wei Shi,Haitao Yu,Zhenwei Feng,Zongjie Wei,Weiyang He,Xin Gou,Yongpeng Xie

STAR protocols 6:103890 PubMed40516044

2025

Protocol for the visualization and identification of S-palmitoylated proteins in HCT-116 cells using metabolic labeling via click chemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Merz,Sabine Grösch

Molecular neurobiology 62:11099-11111 PubMed40261603

2025

Plasma-Derived Small Extracellular Vesicles miR- 182 - 5p Is a Potential Biomarker for Diagnosing Major Depressive Disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Lin Zhu,Lian-Di Li,Xuan-Yu Lin,Jian Hu,Chun Wang,Yi-Jun Wang,Qi-Gang Zhou,Jing Zhang

Journal of nanobiotechnology 22:493 PubMed39160590

2024

Advancing osteoarthritis therapy with GMOCS hydrogel-loaded BMSCs-exos.

Applications

Unspecified application

Species

Unspecified reactive species

Renyi Zhou,Jiarong Guo,Zhe Jin

BMC cancer 24:883 PubMed39039505

2024

Bone marrow mesenchymal stem cells-derived exosomal miR-145-5p reduced non-small cell lung cancer cell progression by targeting SOX9.

Applications

Species

Human

Wu Yan,Haiyu Yang,Dekun Duan,Yufeng Wu,Youhu Liu,Jianping Mao,Yong Zhao,Junsong Ye

Bioactive materials 34:138-149 PubMed38223538

2024

Oral TNF-α siRNA delivery via milk-derived exosomes for effective treatment of inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Geonhee Han,Hyosuk Kim,Hochung Jang,Eun Sun Kim,Sun Hwa Kim,Yoosoo Yang

Medicine 102:e36582 PubMed38065867

2023

Bone Marrow Stromal Cells inhibited the growth and metastasis of human U87 cells through delivering exosomal miR-506.

Applications

Unspecified application

Species

Unspecified reactive species

Liexiang Zhang,Yu Ding,Wei Zhou,Xiaohong Xu,Jing Zheng

Regenerative therapy 25:35-48 PubMed38058606

2023

Epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs attenuates spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Xu,Zhenxiao Ren,Tianzuo Niu,Siyuan Li

Organogenesis 19:2285836 PubMed38031805

2023

Regulation of Interferon-β-Modified Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes in Proliferation and Apoptosis of Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haichao Yuan,Senmao Li,Zhengping Zhao,Yan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Calnexin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB227310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB227310)

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody (AB227310)

Western blot - Anti-Calnexin antibody (AB227310)

Western blot - Anti-Calnexin antibody (AB227310)

Western blot - Anti-Calnexin antibody (AB227310)

Western blot - Anti-Calnexin antibody (AB227310)

Western blot - Anti-Calnexin antibody (AB227310)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise