AB219644

Anti-Calnexin antibody - C-terminal

Name: Anti-Calnexin antibody - C-terminal (ab219644) | Abcam
Brand: Abcam Limited
SKU: AB219644
Price: 490 USD
Availability: InStock

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(5 Publications)

Anti-Calnexin antibody - C-terminal (ab219644) is a goat polyclonal antibody detecting Calnexin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - C-terminal (AB219644)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - C-terminal (AB219644)

Immunofluorescent analysis of Hepa1-6 cells (4% of PFA-fixed) labeling Calnexin with ab219644 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Calnexin with ab219644 at 5 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Calnexin with ab219644 at 5 μg/ml.

Supplier Data

Western blot - Anti-Calnexin antibody - C-terminal (AB219644)

All lanes:

Western blot - Anti-Calnexin antibody - C-terminal (ab219644) at 1/500 dilution

Lane 1:

NIH 3T3 cell lysates at 50 µg

Lane 2:

RAW 264.7 cell lysates at 50 µg

Lane 3:

Hepa1-6 cell lysates at 50 µg

Secondary

All lanes:

Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution

Predicted band size: 68 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CANX aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P27824

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Calnexin antibody - C-terminal (ab219644) is a goat polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of Calnexin?
Anti-Calnexin - C-terminal (ab219644) specifically detects a band for Calnexin (UniProt: P27824) at a molecular weight of 68kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4393 PubMed40355429

2025

SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Stella Hartmann,Lisa Radochonski,Chengjin Ye,Luis Martinez-Sobrido,Jueqi Chen

JCI insight 8: PubMed37014698

2023

Polarized localization of phosphatidylserine in the endothelium regulates Kir2.1.

Applications

Unspecified application

Species

Unspecified reactive species

Claire A Ruddiman,Richard Peckham,Melissa A Luse,Yen-Lin Chen,Maniselvan Kuppusamy,Bruce A Corliss,P Jordan Hall,Chien-Jung Lin,Shayn M Peirce,Swapnil K Sonkusare,Robert P Mecham,Jessica E Wagenseil,Brant E Isakson

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2105320 PubMed35748162

2022

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Applications

Unspecified application

Species

Unspecified reactive species

Hak Park,Soo Kyung Seo,Ju-Ri Sim,Su Jin Hwang,Ye Jin Kim,Dong Hoon Shin,Dong Geon Jang,Shin Hye Noh,Pil-Gu Park,Si Hwan Ko,Mi Hwa Shin,Jae Young Choi,Yukishige Ito,Chung-Min Kang,Jae Myun Lee,Min Goo Lee

mBio 13:e0271721 PubMed35038927

2022

Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-An Kung,Huan-Jung Chiang,Mei-Ling Li,Yu-Nong Gong,Hsin-Ping Chiu,Chuan-Tien Hung,Peng-Nien Huang,Sheng-Yu Huang,Pei-Yu Wang,Tsu-An Hsu,Gary Brewer,Shin-Ru Shih

Nature communications 10:2914 PubMed31266968

2019

The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tongde Du,Hongchang Li,Yongsheng Fan,Lin Yuan,Xiaodan Guo,Qiong Zhu,Yuying Yao,Xin Li,Chunlei Liu,Xinhe Yu,Zhaofei Liu,Chun-Ping Cui,Chuanchun Han,Lingqiang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calnexin antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - C-terminal (AB219644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)

Western blot - Anti-Calnexin antibody - C-terminal (AB219644)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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