Anti-Calnexin antibody [CANX/1543]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [CANX/1543] (AB238078)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for Calnexin using ab238078 at 2 μg/ml in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Recombinant Anti-ETS2 antibody [EPR22419] ab219948 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 53 kDa in Wild-type THP-1 Nuclear cell lysates with no signal observed at this size in ETS2 knockout THP-1 Nuclear cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ETS2 antibody [EPR22419] (<a href='/en-us/products/primary-antibodies/ets2-antibody-epr22419-ab219948'>ab219948</a>) at 1/1000 dilution

Lane 1:

Wild-type THP-1 Nuclear at 40 µg

Lane 2:

Western blot - Human ETS2 knockout THP1 cell line (<a href='/en-us/products/cell-lines/human-ets2-knockout-thp1-cell-line-ab290239'>ab290239</a>) at 40 µg

Lane 3:

K-562 Nuclear at 20 µg

Lane 4:

T-47D Nuclear at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-RET antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 150 kDa in Wild-type MCF7 UNBOILED cell lysates with no signal observed at this size in RET knockout MCF7 UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Ret (E1N8X) XP Rabbit mAb at 1/1000 dilution

Lane 1:

Wild-type MCF7 UNBOILED at 20 µg

Lane 2:

Western blot - Human RET knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-ret-knockout-mcf7-cell-line-ab286279'>ab286279</a>) at 20 µg

Lane 3:

THP-1 UNBOILED at 20 µg

Lane 4:

HepG2 UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 150 kDa,175 kDa

Observed band size: 150 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-FAM38A/PIEZO1 antibody [EPR23826-103] ab259949 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 240-350 kDa in Wild-type A549 not boiled cell lysates with no signal observed at this size in PIEZO1 knockout A549 not boiled cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (<a href='/en-us/products/primary-antibodies/fam38a-piezo1-antibody-epr23826-103-ab259949'>ab259949</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 not boiled at 20 µg

Lane 2:

PIEZO1 knockout A549 not boiled at 20 µg

Lane 3:

Wild-type HeLa not boiled at 20 µg

Lane 4:

PIEZO knockout HeLa not boiled at 20 µg

Lane 5:

A431 not boiled at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 240-350 kDa

Observed band size: 240-350 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Recombinant Anti-ETS2 antibody [EPR22419] ab219948 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 53 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ETS2 antibody [EPR22419] (<a href='/en-us/products/primary-antibodies/ets2-antibody-epr22419-ab219948'>ab219948</a>) at 1/1000 dilution

Lane 1:

Wild-type THP-1 Nuclear Fraction at 40 µg

Lane 2:

Western blot - Human ETS2 knockout THP1 cell line (<a href='/en-us/products/cell-lines/human-ets2-knockout-thp1-cell-line-ab290239'>ab290239</a>) at 40 kDa

Lane 3:

K562 Nuclear Fraction at 20 µg

Lane 4:

T-47D Nuclear Fraction at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : anti-FANCM antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 190 kDa in Wild-type A549 cell lysates with no signal observed at this size in FANCM knockout A549 cell line (ab326031). We are unable to identify the non-specific band at 71kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot at 1/1000 dilution

Lane 1:

Wild-type HCT 116 at 20 µg

Lane 2:

Western blot - Human FANCM knockout A549 cell line (<a href='/en-us/products/cell-lines/human-fancm-knockout-a549-cell-line-ab326031'>ab326031</a>) at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

Jurkat Cytoplasmic at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Polyclonal to CDON / Cdo ab227056 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 170 kDa in Wild-type A549 UNBOILED cell lysates with no signal observed at this size in CDON knockout A549 UNBOILED cell line (ab326028).

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CDON / Cdo antibody - N-terminal (<a href='/en-us/products/primary-antibodies/cdon-cdo-antibody-n-terminal-ab227056'>ab227056</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 UNBOILED at 20 µg

Lane 2:

Western blot - Human CDON knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cdon-knockout-a549-cell-line-ab326028'>ab326028</a>) at 20 µg

Lane 3:

HEK-293T UNBOILED at 20 µg

Lane 4:

HepG2 UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 170 kDa

Observed band size: 170 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit monoclonal [EPR23442-43] to BRCA2 ab239375 staining at 1/500 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 270, 400 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in BRCA2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 2:

Western blot - Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/brca2-antibody-epr23442-43-bsa-and-azide-free-ab273157'>ab273157</a>) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-BRCA2 antibody [EPR23442-43] (<a href='/en-us/products/primary-antibodies/brca2-antibody-epr23442-43-ab239375'>ab239375</a>) at 1/500 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

BRCA2 knockout MCF7

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 270 kDa,400 kDa

Observed band size: 270 kDa,400 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[EPR23110-46] to Fibronectin ab268020 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 238-268 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in FN1 knockout MCF7 cell line (ab326019). To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Fibronectin antibody [EPR23110-46] (<a href='/en-us/products/primary-antibodies/fibronectin-antibody-epr23110-46-ab268020'>ab268020</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human FN1 knockout MCF7 cell line (ab286324) at 20 µg

Lane 2:

Western blot - Human FN1 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-fn1-knockout-mcf7-cell-line-ab326019'>ab326019</a>) at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 272 kDa

Observed band size: 238 kDa,268 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[F14] to Fibronectin ab45688 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 238-268 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in FN1 knockout MCF7 cell line (ab326019). To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Fibronectin antibody [F14] (<a href='/en-us/products/primary-antibodies/fibronectin-antibody-f14-ab45688'>ab45688</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human FN1 knockout MCF7 cell line (ab286324) at 20 µg

Lane 2:

Western blot - Human FN1 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-fn1-knockout-mcf7-cell-line-ab326019'>ab326019</a>) at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 272 kDa

Observed band size: 238-268 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit monoclonal [EPR24303-10] to C5a ab281923 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 180 kDa in Wild-type A549 cell lysates with no signal observed at this size in C5 knockout A549 cell line (ab326016). To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-C5a antibody [EPR24303-10] (<a href='/en-us/products/primary-antibodies/c5a-antibody-epr24303-10-ab281923'>ab281923</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human C5 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-c5-knockout-a549-cell-line-ab326016'>ab326016</a>) at 20 µg

Lane 2:

C5 knockout A549 at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 188 kDa

Observed band size: 180 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[UDD3 30(12)] to LRRK2 ab133518 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 268 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in LRRK2 knockout U-87 MG cell line (ab306722). To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LRRK2 antibody [UDD3 30(12)] (<a href='/en-us/products/primary-antibodies/lrrk2-antibody-udd3-3012-ab133518'>ab133518</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human LRRK2 knockout U-87 MG cell line (<a href='/en-us/products/cell-lines/human-lrrk2-knockout-u-87-mg-cell-line-ab306722'>ab306722</a>) at 20 µg

Lane 3:

A549 at 20 µg

Lane 4:

HT-29 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 286 kDa

Observed band size: 268 kDa,80 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-EIF4A2 antibody [EPR27347-74] (ab307728) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab307728 was shown to bind specifically to EIF4A2. A band was observed at 48 kDa in wild-type A549 cell lysates with no signal observed at this size in EIF4A2 knockout cell line (ab326029). To generate this image, wild-type and EIF4A2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-eIF4A2 antibody [EPR27347-74] (<a href='/en-us/products/primary-antibodies/eif4a2-antibody-epr27347-74-ab307728'>ab307728</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human EIF4A2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-eif4a2-knockout-a549-cell-line-ab326029'>ab326029</a>) at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

MCF7 Membrane Prep cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 48 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Polyclonal to CDON / Cdo ab227056 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 170 kDa in Wild-type A549 UNBOILED cell lysates with no signal observed at this size in CDON knockout A549 UNBOILED cell line (ab326028).

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 100pc LICOR in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CDON / Cdo antibody - N-terminal (<a href='/en-us/products/primary-antibodies/cdon-cdo-antibody-n-terminal-ab227056'>ab227056</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 UNBOILED at 20 µg

Lane 2:

Western blot - Human CDON knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cdon-knockout-a549-cell-line-ab326028'>ab326028</a>) at 20 µg

Lane 3:

HEK-293T UNBOILED at 20 µg

Lane 4:

HepG2 UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 140 kDa

Observed band size: 170 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-CTGF antibody [EPR20728] ab209780 staining at 1/200 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 37 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CTGF antibody [EPR20728] (<a href='/en-us/products/primary-antibodies/ctgf-antibody-epr20728-ab209780'>ab209780</a>) at 1/200 dilution

Lane 1:

Wild-type A549 BFA (5 g/mL, 6 h) at 20 µg

Lane 2:

Wild-type A549 BFA (0 g/mL, 6 h) at 20 µg

Lane 3:

CCN2 knockout A549 BFA (5 g/mL, 6 h) at 20 µg

Lane 4:

CCN2 knockout A549 BFA (0 g/mL, 6 h) at 20 µg

Lane 5:

U-2 OS at 20 µg

Lane 6:

HepG2 at 20 µg

Lane 7:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-PHLPP1 antibody [EPR27151-55] ab305295 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 160-200 kDa in Wild-type A549 cell lysates with no signal observed at this size in PHLPP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (<a href='/en-us/products/primary-antibodies/phlpp1-antibody-epr27151-55-ab305295'>ab305295</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 40 µg

Lane 2:

Western blot - Human PHLPP1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-phlpp1-knockout-a549-cell-line-ab287700'>ab287700</a>) at 40 µg

Lane 3:

HEK-293 at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 160-200 kDa

Observed band size: 160-200 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-CTGF antibody [RM1199] ab318148 staining at 1/2000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 37 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CTGF antibody [RM1199] (<a href='/en-us/products/primary-antibodies/ctgf-antibody-rm1199-ab318148'>ab318148</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 BFA (5 g/mL, 6 h) at 20 µg

Lane 2:

Wild-type A549 BFA (0 g/mL, 6 h) at 20 µg

Lane 3:

CCN2 knockout A549 BFA (5 g/mL, 6 h) at 20 µg

Lane 4:

CCN2 knockout A549 BFA (0 g/mL, 6 h) at 20 µg

Lane 5:

U-2 OS at 20 µg

Lane 6:

HepG2 at 20 µg

Lane 7:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-GTPase HRAS antibody [Y132] ab32417 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 22 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in HRAS knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-GTPase HRAS antibody [Y132] (<a href='/en-us/products/primary-antibodies/gtpase-hras-antibody-y132-ab32417'>ab32417</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 whole cell lysate at 20 µg

Lane 2:

HRAS knockout MCF7 whole cell lysate at 20 µg

Lane 3:

HEK-293 whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-TRF1 antibody [EPR28580-71] ab323380 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in TERF1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TRF1 antibody [EPR28580-71] (<a href='/en-us/products/primary-antibodies/trf1-antibody-epr28580-71-ab323380'>ab323380</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 whole cell lysate at 20 µg

Lane 2:

TERF1 knockout A549 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

HEK-293 whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 55 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-SIRT2 antibody [EP1668Y] ab51023 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 23, 36, 40, 45 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in SIRT2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SIRT2 antibody [EP1668Y] (<a href='/en-us/products/primary-antibodies/sirt2-antibody-ep1668y-ab51023'>ab51023</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 whole cell lysate at 20 µg

Lane 2:

Western blot - Human SIRT2 knockout MCF7 cell line (ab289308) at 20 µg

Lane 3:

THP-1 whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Lane 5:

U-2 OS whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 23 kDa,36 kDa,40 kDa,45 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-p53 antibody [E26] ab32389 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 50 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in TP53 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-p53 antibody [E26] (<a href='/en-us/products/primary-antibodies/p53-antibody-e26-ab32389'>ab32389</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 whole cell lysate at 20 µg

Lane 2:

Western blot - Human TP53 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-tp53-knockout-mcf7-cell-line-ab286440'>ab286440</a>) at 20 µg

Lane 3:

Wild-type A549 whole cell lysate at 20 µg

Lane 4:

TP53 knockout A549 whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-p53 antibody [EPR17343] ab179477 staining at 1/2000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 50 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in TP53 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-p53 antibody [EPR17343] (<a href='/en-us/products/primary-antibodies/p53-antibody-epr17343-ab179477'>ab179477</a>) at 1/2000 dilution

Lane 1:

Wild-type MCF7 whole cell lysate at 20 µg

Lane 2:

Western blot - Human TP53 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-tp53-knockout-mcf7-cell-line-ab286440'>ab286440</a>) at 20 µg

Lane 3:

Wild-type A549 whole cell lysate at 20 µg

Lane 4:

TP53 knockout A549 whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-TSC2 antibody [EP1107Y] (ab52936) staining at 1/20000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52936 detected a band observed at 200 kDa in wild-type MCF7 cell lysates with no change observed in the TSC2 knockout cell line ab286525. To generate this image, wild-type and TSC2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Tuberin antibody [EP1107Y] (<a href='/en-us/products/primary-antibodies/tuberin-antibody-ep1107y-ab52936'>ab52936</a>) at 1/20000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human TSC2 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-tsc2-knockout-mcf7-cell-line-ab286525'>ab286525</a>) at 20 µg

Lane 2:

TSC2 knockout MCF7 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

TSC2 knockout HAP1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 201 kDa

Observed band size: 200 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-CP antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 140-150 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Ceruloplasmin (D7Q5W) Rabbit mAb at 1/1000 dilution

Lane 1:

Wild-type A549 Treated BFA (5ug/mL, 6h) at 20 µg

Lane 2:

Wild-type A549 Vehicle Control BFA (0ug/mL, 6h) at 20 µg

Lanes 3 - 4:

Western blot - Human CP Knockout A549 cell line (ab314964) at 20 µg

Lane 5:

SK-OV-3 at 20 µg

Lane 6:

HeLa at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 122 kDa

Observed band size: 140-150 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-NUAK2 antibody [EPR28627-717] ab322265 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 75 kDa in Wild-type A549 cell lysates with no signal observed at this size in NUAK2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-NUAK2 antibody [EPR28627-717] (<a href='/en-us/products/primary-antibodies/nuak2-antibody-epr28627-717-ab322265'>ab322265</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

NUAK2 knockout A549 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 75 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[EPR25194-152] to P2X4 ab303496 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 58, 73 kDa in Wild-type A549 cell lysates with no signal observed at this size in P2RX4 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-P2X4 antibody [EPR25194-152] (<a href='/en-us/products/primary-antibodies/p2x4-antibody-epr25194-152-ab303496'>ab303496</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

P2RX4 knockout A549 at 20 µg

Lane 3:

Wild-type HEK-293T BOILED at 20 µg

Lane 4:

P2RX4 knockout HEK-293T BOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 58 kDa,73 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-PHLPP1 antibody [EPR27151-55] ab305295 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 160-200 kDa in Wild-type A549 cell lysates with no signal observed at this size in PHLPP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (<a href='/en-us/products/primary-antibodies/phlpp1-antibody-epr27151-55-ab305295'>ab305295</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 40 µg

Lane 2:

Western blot - Human PHLPP1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-phlpp1-knockout-a549-cell-line-ab287700'>ab287700</a>) at 40 µg

Lane 3:

HEK-293 at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 185 kDa

Observed band size: 160-200 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-SIRT2 antibody [EPR1667] ab134171 staining at 1/500 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 45 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in SIRT2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SIRT2 antibody [EPR1667] (<a href='/en-us/products/primary-antibodies/sirt2-antibody-epr1667-ab134171'>ab134171</a>) at 1/500 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human SIRT2 knockout MCF7 cell line (ab289308) at 20 µg

Lane 3:

THP-1 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[EPR4033] to BNIP3L/NIX ab109414 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 26-36 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (<a href='/en-us/products/primary-antibodies/bnip3l-nix-antibody-epr4033-ab109414'>ab109414</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 0.1mM CoCl2 24h at 20 µg

Lane 2:

Wild-type A549 Vehicle Control 0mM CoCl2 24h at 20 µg

Lane 3:

BNIP3L knockout A549 0.1mM CoCl2 24h at 20 µg

Lane 4:

BNIP3L knockout A549 Vehicle Control 0mM CoCl2 24h at 20 µg

Lane 5:

HeLa 0.1mM CoCl2 24h at 20 µg

Lane 6:

HeLa Vehicle Control 0mM CoCl2 24h at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 26-36 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-Perilipin 2 antibody ab78920 staining at 1 µg/mL, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in PLIN2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Perilipin 2 antibody (<a href='/en-us/products/primary-antibodies/perilipin-2-antibody-ab78920'>ab78920</a>) at 1 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

PLIN2 knockout A549 at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

SK-OV-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 55 kDa,95 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-MED12 antibody [EPR25211-36] ab300154 staining at 1/2000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 243 kDa in Wild-type HCT116 cell lysates with no signal observed at this size in MED12 knockout HCT116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-MED12 antibody [EPR25211-36] (<a href='/en-us/products/primary-antibodies/med12-antibody-epr25211-36-ab300154'>ab300154</a>) at 1/2000 dilution

Lane 1:

Wild-type HCT116 at 20 µg

Lane 2:

Western blot - Human MED12 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-med12-knockout-hct116-cell-line-ab286465'>ab286465</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

A549 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 243 kDa

Observed band size: 243 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-SIRT2 antibody [EPR20411-105] ab211033 staining at 1/5000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 45 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in SIRT2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SIRT2 antibody [EPR20411-105] (<a href='/en-us/products/primary-antibodies/sirt2-antibody-epr20411-105-ab211033'>ab211033</a>) at 1/5000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human SIRT2 knockout MCF7 cell line (ab289308) at 20 µg

Lane 3:

THP-1 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

All lanes:

Western blot - Anti-C5 / C5b antibody [EPR23940-3] (<a href='/en-us/products/primary-antibodies/c5-c5b-antibody-epr23940-3-ab275931'>ab275931</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 ab288558 at 20 µg

Lane 2:

Western blot - Human C5 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-c5-knockout-a549-cell-line-ab326016'>ab326016</a>) at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 188 kDa

Observed band size: 180 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-CEACAM5 antibody (ab131070) staining at 0.1 ug/ml, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab131070 was shown to bind specifically to CEACAM5. A band was observed at 170 kDa in wild-type A549 cell lysates with no signal observed at this size in CEACAM5 knockout cell line. To generate this image, wild-type and CEACAM5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CEACAM5 antibody (<a href='/en-us/products/primary-antibodies/carcino-embryonic-antigen-cea-antibody-ab131070'>ab131070</a>) at 0.1 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human CEACAM5 knockout A549 cell line (ab287319)

Lane 2:

CEACAM5 knockout A549 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

PANC-1 cell lysate at 20 µg

Predicted band size: 76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

All lanes:

Western blot - Anti-ATP7b antibody [EPR6794] (<a href='/en-us/products/primary-antibodies/atp7b-antibody-epr6794-ab124973'>ab124973</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 10 µg

Lane 2:

Western blot - Human ATP7B knockout A549 cell line (<a href='/en-us/products/cell-lines/human-atp7b-knockout-a549-cell-line-ab326074'>ab326074</a>) at 10 µg

Lane 3:

HepG2 at 10 µg

Lane 4:

Ramos at 10 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 157 kDa

Observed band size: 153 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : HRP Anti-ALDH5A1/SSADH antibody [EPR7794] (ab314408) staining at 1/1000 dilution, shown in black; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab314408 was shown to bind specifically to ALDH5A1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibody Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 8 minutes exposure time.

All lanes:

Western blot - HRP Anti-ALDH5A1/SSADH antibody [EPR7794] (<a href='/en-us/products/primary-antibodies/hrp-aldh5a1-ssadh-antibody-epr7794-ab314408'>ab314408</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 54 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal [EPR23044-100] to RUNX1 / AML1 ab240639 staining at a 1/200 dilution, shown in black; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 50-54 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in RUNX1 knockout U-87 MG cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 4:

Western blot - Anti-RUNX1 / AML1 antibody [EPR23044-100] (<a href='/en-us/products/primary-antibodies/runx1-aml1-antibody-epr23044-100-ab240639'>ab240639</a>) at 1/200 dilution

Lanes 1 - 4:

Western blot - Anti-RUNX1 / AML1 antibody [EPR23044-100] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/runx1-aml1-antibody-epr23044-100-bsa-and-azide-free-ab264471'>ab264471</a>) at 1/200 dilution

Lane 1:

Wild-type U-87 MG at 40 µg

Lane 2:

Western blot - Human RUNx1 knockout U-87 MG cell line (ab306773) at 40 µg

Lane 3:

Jurkat at 20 µg

Lane 4:

Caco-2 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 50-54 kDa

true

Exposure time: 10s

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab314937) staining at 1/2000 dilution, shown in black; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta.

In Western blot, ab314937 was shown to bind specifically to NKX2-1/TTF-1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit.

Secondary antibodies used were HRP conjugated Streptavidin at 0.1 µg/ml and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Biotin Anti-TTF1/Nkx2-1 antibody [EP1584Y] (<a href='/en-us/products/primary-antibodies/biotin-ttf1-nkx2-1-antibody-ep1584y-ab314937'>ab314937</a>) at 1/2000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

HEK-293 cell lysate at 20 µg

Secondary

Lane 1:

HRP conjugated Streptavidin at 0.1 µg/mL

Lane 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 39 kDa,75 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

False colour image of Western blot : Anti-Chd7 antibody staining at 2 ug/ml, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab117522 was found to be non-specific. A band was observed at 160 kDa in wild-type SH-SY5Y cell lysates with no change observed in the CHD7 knockout cell line ab280067 (knockout cell lysate ab280126). To generate this image, wild-type and CHD7 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Chd7 antibody (<a href='/en-us/products/primary-antibodies/chd7-antibody-ab117522'>ab117522</a>) at 2 µg/mL

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

CHD7 knockout SH-SY5Y cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

DLD-1 cell lysate at 20 µg

Lane 5:

HEK-293 cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lanes 1 - 5:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 336 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

All lanes:

Anti-USP18 antibody at 1/1000 dilution

Lane 1:

Wild-type A549 Vehicle Control IFNa (0 IU/mL, 12 h) cell lysate at 20 µg

Lane 2:

Wild-type A549 Treated IFNa (100 IU/mL, 12 h) cell lysate at 20 µg

Lane 3:

USP18 knockout A549 Vehicle Control IFNa (0 IU/mL, 12 h) ab262530 cell lysate at 20 µg

Lane 4:

USP18 knockout A549 Treated IFNa (100 IU/mL, 12 h) ab262530 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 34-37 kDa

false