AB213243

Anti-Calnexin antibody [EPR21205]

Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(15 Publications)

Rabbit Recombinant Monoclonal Calnexin antibody. Suitable for WB and reacts with Human samples. Cited in 15 publications.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

1 Images

Lab

Western blot - Anti-Calnexin antibody [EPR21205] (AB213243)

Lane 1 : NIH3T3 whole cell lysate (10 μg)
Lane 2 : Mouse brain whole tissue lysate (10 μg)
Lane 3 : HeLa whole cell lysate (10 μg)
Lane 4 : Hap1 Wild-type whole cell lysate (10 μg)
Lane 5 : CANX knockout Hap1 whole cell lysate (10 μg)

Lanes 1 - 5 : Merged signal (red and green). Green - ab213243 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab213243 was shown to specifically react with Calnexin in wild-type HAP1 cells as signal was lost in Calnexin (CANX) knockout cells. Wild-type and CANX knockout samples were subjected to SDS-PAGE. Nitrocellulose membranes were blocked in 3% milk in TBST before ab213243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1μg/mL and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)

Predicted band size: 68 kDa

Observed band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21205

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2.5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.05 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.05 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cirugia y cirujanos 93:151-157 PubMed40199318

2025

Evaluation of the effectiveness of human breast milk exosomes in experimental testicular torsion-detorsion injury model.

Applications

Unspecified application

Species

Unspecified reactive species

Ünal T Öztürk,Hatice S Y Cömert,Gül Şalcı,Ahmet Alver,Sevdegül A Mungan,Neslihan Sağlam,Şeniz Erdem,Serdar Karakullukçu,Mustafa İmamoğlu,Haluk Sarıhan

Cell communication and signaling : CCS 23:96 PubMed39966859

2025

REG3A secreted by peritumoral acinar cells enhances pancreatic ductal adenocarcinoma progression via activation of EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Ren,Yunfei Teng,Kunxin Xie,Xiao He,Gang Chen,Kaini Zhang,Qingyi Liao,Jia Zhang,Xiaohang Zhou,Yating Zhu,Wenyu Song,Yuege Lin,Yi Zhang,Zhijian Xu,Noriaki Maeshige,Xiubin Liang,Dongming Su,Peng Sun,Ying Ding

Bioactive materials 36:508-523 PubMed39072285

2024

Macrophage-derived extracellular vesicles regulate skeletal stem/progenitor Cell lineage fate and bone deterioration in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Chen He,Chen Hu,Wen-Zhen He,Yu-Chen Sun,Yangzi Jiang,Ling Liu,Jing Hou,Kai-Xuan Chen,Yu-Rui Jiao,Mei Huang,Min Huang,Mi Yang,Qiong Lu,Jie Wei,Chao Zeng,Guang-Hua Lei,Chang-Jun Li

Journal of extracellular biology 3:e146 PubMed38939414

2024

Characterising the HLA-I immunopeptidome of plasma-derived extracellular vesicles in patients with melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin Boyne,Abigail Coote,Silvia Synowsky,Aaron Naden,Sally Shirran,Simon J Powis

Journal of extracellular vesicles 13:e12465 PubMed38887984

2024

Stress-induced Rab11a-exosomes induce amphiregulin-mediated cetuximab resistance in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

John D Mason,Ewan Marks,Shih-Jung Fan,Kristie McCormick,Clive Wilson,Adrian L Harris,Freddie C Hamdy,Chris Cunningham,Deborah C I Goberdhan

Current issues in molecular biology 46:1593-1606 PubMed38392221

2024

Plastoquinone-Derivative SkQ1 Improved the Biliary Intraepithelial Neoplasia during Liver Fluke Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Oxana Zaparina,Anna Kovner,Viktoria Petrova,Nataliya Kolosova,Viatcheslav Mordvinov,Maria Pakharukova

Frontiers in endocrinology 14:1149168 PubMed37124755

2023

Bone marrow mesenchymal stem cells derived exosomal miRNAs can modulate diabetic bone-fat imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Han,Chao Wang,Peng Cheng,Ting Liu,Wei-Shan Wang

Journal of nanobiotechnology 21:88 PubMed36915132

2023

Mechanical force induces macrophage-derived exosomal UCHL3 promoting bone marrow mesenchymal stem cell osteogenesis by targeting SMAD1.

Applications

Unspecified application

Species

Unspecified reactive species

Panjun Pu,Shengnan Wu,Kejia Zhang,Hao Xu,Jiani Guan,Zhichun Jin,Wen Sun,Hanwen Zhang,Bin Yan

Biochemistry and biophysics reports 31:101299 PubMed35812347

2022

Exosome-mediated transduction of mechanical force regulates prostate cancer migration via microRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiao Liu,Qishu Jin,Taofei Yan,Yinqing Wo,Huiwen Liu,Yue Wang

Immunology 166:249-264 PubMed35318648

2022

Tumour-associated antigenic peptides are present in the HLA class I ligandome of cancer cell line derived extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Pankaj Kumar,Caitlin Boyne,Sydney Brown,Ayesha Qureshi,Peter Thorpe,Silvia A Synowsky,Sally Shirran,Simon J Powis

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calnexin antibody [EPR21205]

Western blot - Anti-Calnexin antibody [EPR21205] (AB213243)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise