AB241154

Anti-Calnexin antibody [EPR21240] - ER Membrane Marker

20ul selling size
KO Validated
RabMAb
Recombinant
Lab Essentials
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(3 Publications)

Rabbit Recombinant Monoclonal Calnexin antibody. Endoplasmic Reticulum Membrane marker. Suitable for WB and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

1 Images

Western blot - Anti-Calnexin antibody [EPR21240] - ER Membrane Marker (AB241154)

WB

Lab

Western blot - Anti-Calnexin antibody [EPR21240] - ER Membrane Marker (AB241154)

ab241154 was shown to specifically react with CANX (Calnexin) in wild type HAP1 cells. No band was observed when CANX (calnexin) knockout samples were used. Wild-type and CANX (calnexin) knockout samples were subjected to SDS-PAGE. ab241154 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/ml dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Calnexin antibody [EPR21240] - ER Membrane Marker (ab241154)

Lane 1:

HAP1 whole cell lysate at 20 µg

Lane 2:

HAP1 CANX KO whole cell lysate at 20 µg

Lane 3:

NIH3T3 whole cell lysate at 20 µg

Lane 4:

MEF1 whole cell lysate at 20 µg

Predicted band size: 68 kDa

Observed band size: 80 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21240

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends a 5% milk block for this product.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends a 5% milk block for this product.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental pharmacology & physiology 52:e70079 PubMed41022671

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Kisspeptin-10 Preserves the Blood-Brain Barrier's Integrity Post-Stroke by Augmenting Claudin-10 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Li,Yunlong Guo,Kaijie Zhao

Oxidative medicine and cellular longevity 2022:8825784 PubMed35281474

2022

BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP Delivery of miR-877-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Dapeng Feng,Zhengwei Li,Liang Yang,Haidong Liang,Hongtao He,Lin Liu,Wei Zhang

Cancer cell international 21:485 PubMed34521413

2021

Engineered exosomes loaded with miR-449a selectively inhibit the growth of homologous non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Mingming Xu,Zhipeng Wang,Mingjun Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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