AB324471

Anti-Calnexin antibody [EPR28028-114] - BSA and Azide free (Detector)

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal Calnexin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

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Sandwich ELISA - Anti-Calnexin antibody [EPR28028-114] - BSA and Azide free (Detector) (AB324471)

sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-114] - BSA and Azide free (Detector) (AB324471)

Interpolated concentration of native Calnexin was measured at different sample concentrations. Undiluted samples are as follows : HAP1 cell extract (3.1 µg/mL) and HEK293T cell extract (2.5 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-114] - BSA and Azide free (Detector) (AB324471)

Interpolated concentrations of native Calnexin in human control wild type HEK293T (human embryonic kidney epithelial cell) and CANX (Calnexin) knockout HEK293T cell based on 2.5 µg/mL extract loads. The concentrations of Calnexin were measured and interpolated from the Calnexin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The Calnexin concentration was determined to be 6233.8 pg/mL in wild type HEK293T extract and undetectable in CANX (Calnexin) knockout HEK293T extract (Human CANX (Calnexin) knockout HEK293T cell line ab255368).

sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-114] - BSA and Azide free (Detector) (AB324471)

Sandwich ELISA with the capture antibody ab324470 dilution at 2 μg/mL and detector antibody ab324471 dilution at 0.5 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-114] - BSA and Azide free (Detector) (AB324471)

Interpolated concentrations of native Calnexin in human control wild type HAP1 (human near-haploid cell) and CANX (Calnexin) knockout HAP1 cell based on 3.1 μg/mL extract loads. The concentrations of Calnexin were measured in duplicate and interpolated from the Calnexin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Calnexin concentration was determined to be 3829.6 pg/mL in wild type HAP1 extract and undetectable in CANX (Calnexin) knockout HAP1 extract.

sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-114] - BSA and Azide free (Detector) (AB324471)

Interpolated concentration of native Calnexin was measured at different sample concentrations. Undiluted samples are as follows : human serum 100% and human plasma (EDTA) 100%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28028-114

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab324471 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Calnexin. The recommended pair for sandwich ELISA is:
Capture: ab324470, Human Calnexin Capture Antibody (unconjugated)
Detector: ab324471, Human Calnexin Detector Antibody (unconjugated)
The reference range value is 0.312-10 ng/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com