Anti-Calnexin antibody - ER Marker (ab22595) is a rabbit polyclonal antibody detecting Calnexin in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 480 publications
- Trusted since 2006
Images
Publications
Filter by
- Advances in clinical and experimental medicine : official organ Wroclaw Medical University 33:499-5102024Human umbilical cord mesenchymal stem cell-derived exosomal miR-214-3p regulates the progression of gallbladder cancer by regulating ACLY/GLUT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuyao Liu,Wang Xiao,Zhulin Yang,Qunwei Wang,Jianing YiPubMed 37747441
- Journal of translational medicine 21:9272023Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofang Zhou,Mu Liu,Lijuan Sun,Yumei Cao,Shanmei Tan,Guangxia Luo,Tingting Liu,Ying Yao,Wangli Xiao,Ziqing Wan,Jie TangPubMed 38129848
- Journal of orthopaedic surgery and research 18:9372023Exosomal miR-182 derived from bone marrow mesenchymal stem cells drives carfilzomib resistance of multiple myeloma cells by targeting SOX6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShifeng Long,Shengping Long,Honglei He,Liang Luo,Mei Liu,Ting DingPubMed 38062424
- Biomolecules & biomedicine :2023Mechanism of adipose tissue-derived stromal cell-extracellular vesicles in treating oral submucous fibrosis by blocking the TGF-β1/Smad3 pathway via the miR-760-3p/IGF1R axisApplications:Unspecified applicationReactive species:Unspecified reactive speciesFengcong Wang,Li Jiang,Ping Liu,Yanjun JiangPubMed 38059910
- Journal of bioenergetics and biomembranes 56:31-442023Chondroprotective effects of bone marrow mesenchymal stem cell-derived exosomes in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi Cheng,Xiangning Xu,Ren Wang,Weijie Chen,Kunhan Qin,Jinglong YanPubMed 38012335
- Scientific reports 13:204672023ALS/FTD-associated mutation in cyclin F inhibits ER-Golgi trafficking, inducing ER stress, ERAD and Golgi fragmentation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAudrey M G Ragagnin,Vinod Sundaramoorthy,Fabiha Farzana,Shashi Gautam,Sayanthooran Saravanabavan,Zeinab Takalloo,Prachi Mehta,Dzung Do-Ha,Sonam Parakh,Sina Shadfar,Julie Hunter,Marta Vidal,Cyril J Jagaraj,Mariana Brocardo,Anna Konopka,Shu Yang,Stephanie L Rayner,Kelly L Williams,Ian P Blair,Roger S Chung,Albert Lee,Lezanne Ooi,Julie D AtkinPubMed 37993492
- Journal of extracellular vesicles 12:e123782023RTP801 mediates transneuronal toxicity in culture via extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJúlia Solana-Balaguer,Núria Martín-Flores,Pol Garcia-Segura,Genís Campoy-Campos,Leticia Pérez-Sisqués,Almudena Chicote-González,Joaquín Fernández-Irigoyen,Enrique Santamaría,Esther Pérez-Navarro,Jordi Alberch,Cristina MalageladaPubMed 37932242
- Journal of nanobiotechnology 21:3982023PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Xie,Yihe Hu,Weiping Su,Sijie Chen,Jiahao Wang,Shuailong Liang,Mingyu Chen,Haoyi Wang,Tianliang MaPubMed 37904168
- International journal of molecular sciences 24:2023The β Isoform of Human ATP-Binding Cassette B5 Transporter, ABCB5β, Localizes to the Endoplasmic Reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdriana María Díaz-Anaya,Louise Gerard,Martine Albert,Jean-François Gaussin,Marielle Boonen,Jean-Pierre GilletPubMed 37958830
- Frontiers in cell and developmental biology 11:12385462023Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Jie Jiang,Song-Hee Lee,Geun Heo,Hak Jae Chung,Eun Seok Cho,Soo Jin Sa,Shinichi Hochi,Xiang-Shun CuiPubMed 37965572
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Common marmoset | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Common marmoset | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/250 | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Common marmoset | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Common marmoset | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Alternative names
Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX
Recommended products
Anti-Calnexin antibody - ER Marker (ab22595) is a rabbit polyclonal antibody detecting Calnexin in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 480 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Recognizes ER membrane, mitochondria and cis-Golgi
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Anti-Calnexin antibody - ER Marker (ab22595) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IP, WB in human samples.
Anti-Calnexin antibody - ER Marker (ab22595) has been cited over 482 times in peer reviewed journals, has been used for calnexin staining and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Calnexin antibody - ER Marker (ab22595) has high sensitivity and specificity.
The specificity of Anti-Calnexin antibody - ER Marker (ab22595) has been confirmed by testing in knockout samples.
Anti-Calnexin antibody - ER Marker (ab22595) has 28 independent reviews from customers.
Anti-Calnexin antibody - ER Marker (ab22595) specifically detects Calnexin (UniProt ID: P27824; Molecular weight: 65kDa) and is sold in 100 µg selling sizes.
Calnexin, an endoplasmic reticulum (ER) chaperone protein, plays a crucial role in oncology by influencing the immune response to tumors and cancer cell behavior. It can impair antitumor immunity by inhibiting T-cell infiltration and enhancing PD-1 expression on T cells, which suppresses their activity. Additionally, aberrant O-glycosylation of calnexin is linked to increased cancer cell migration and adhesion.
Explore our range of organelle-specific antibodies and dyes and discover why our reagents are deeply trusted by cell biologists.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.
- Biological function summary
Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.
- Pathways
Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.
- Associated diseases and disorders
Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (ab22595)
ab22595 staining Calnexin in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab22595 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1 μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120).
Nuclear DNA was labeled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunoprecipitation - Anti-Calnexin antibody - ER Marker (ab22595)
Calnexin was immunoprecipitated using 0.5 mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract, 5 μg of Rabbit polyclonal to Calnexin - ER membrane marker and 50 μl of protein G magnetic beads (+).
No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10 minutes, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
Proteins were eluted by addition of 40 μl SDS loading buffer and incubated for 10 minutes at 70oC; 10 μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22595.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 80kDa: Calnexin - ER membrane marker.All lanes: Immunoprecipitation - Anti-Calnexin antibody - ER Marker (ab22595)
Predicted band size: 68 kDa
Dombert et al PLoS One. 2014 Oct 22;9(10):e110846. doi: 10.1371/journal.pone.0110846. eCollection 2014. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
Subcellular distribution of Smn and hnRNP R in isolated mouse embryonic motoneurons.Lentiviral knockdown of hnRNP R led to a dose-dependent reduction of hnRNP R levels. Calnexin and Smn protein were not altered significantly.
Primary motoneurons or E18 spinal cord tissue, respectively, were lysed with cytosolic and nuclear fractionation buffer, solubilized in Laemmli buffer and boiled for 10 minutes at 99°C. Proteins were then subjected to SDS-PAGE, blotted onto PVDF membrane, incubated with the corresponding antibodies, including ab22595.
All lanes: Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
Predicted band size: 68 kDa
Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: empty lane
Lane 3: CANX knockout HAP1 whole cell lysate (20 μg)
Lane 4: empty lane
Lanes 1 - 4: Merged signal (red and green). Green - ab22595 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab22595 was shown to specifically react with CANX (Calnexin) in wildtype cells as signal was lost in CANX (Calnexin) knockout cells. Wild-type and eCANX (Calnexin) knockout samples were subjected to SDS-PAGE. ab22595 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 dilution and 1/10,000 dilution respectively.Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
Predicted band size: 68 kDa
Observed band size: 80 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (ab22595)
ab22595 staining Calnexin in wild-type HAP1 cells (top panel) and CANX knockout HAP1 cells (bottom panel).
The cells were fixed with 4% formaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab22595 at 1 μg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green).
Nuclear DNA was labeled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Calnexin knockout HAP1 cell lysate (20 μg)Lanes 1 - 2: Merged signal (red and green). Green - ab22595 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
This western blot image is a comparison between ab22595 and a competitor's top cited rabbit polyclonal antibody.
All lanes: Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
Predicted band size: 68 kDa
Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
All lanes: Western blot - Anti-Calnexin antibody - ER Marker (ab22595) at 1/250 dilution
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate (ab27253) at 10 µg
Lane 4: Liver (Mouse) Tissue Lysate (ab7935) at 10 µg
Lane 5: Heart (Mouse) Tissue Lysate (ab27255) at 10 µg
Lane 6: Kidney (Mouse) Tissue Lysate (ab27254) at 10 µg
Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 8: Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 10 µg
Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg
Lane 10: Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg
Lane 11: Ovary (Mouse) Tissue Lysate (ab35808) at 10 µg
Lane 12: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) at 10 µg
Lane 13: Brain (Rat) Tissue Lysate (ab7942) at 10 µg
Lane 14: Liver (Rat) Tissue Lysate (ab27256) at 10 µg
Lane 15: Heart (Rat) Tissue Lysate (ab7940) at 10 µg
Lane 16: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa
Western blot - Anti-Calnexin antibody - ER Marker (ab22595)
Recent batches of ab22595 (AP217379 and AP151845) detect a band of ~ 75 kDa in HeLa, U-2 OS and MCF7 lysates. This band is completely blocked by the immunizing peptide so we believe this represents Calnexin. Moreoever, a band of the same size is detected by other Calnexin antibodies tested.
All lanes: Western blot - Anti-Calnexin antibody - ER Marker (ab22595) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Lane 2: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg
Lane 3: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4: HeLa whole cell lysate at 20 µg
Lane 5: U-2 OS whole cell lysate at 20 µg
Lane 6: MCF7 whole cell lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com