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AB75801

Anti-Calnexin antibody - ER Membrane Marker

5

(1 Review)

|

(87 Publications)

Rabbit Polyclonal Calnexin antibody. Endoplasmic Reticulum Membrane marker. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 87 publications. Immunogen corresponding to Synthetic Peptide within Dog CANX conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Calnexin, pp90, CANX

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Membrane Marker (AB75801)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Membrane Marker (AB75801)

ab75801 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - ER Membrane Marker (AB75801)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - ER Membrane Marker (AB75801)

ab75801 staining Calnexin in juvenile mouse backskin tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Western blot - Anti-Calnexin antibody - ER Membrane Marker (AB75801)
  • WB

Unknown

Western blot - Anti-Calnexin antibody - ER Membrane Marker (AB75801)

All lanes:

Western blot - Anti-Calnexin antibody - ER Membrane Marker (ab75801) at 1/2000 dilution

All lanes:

HeLa cell lysate

Secondary

All lanes:

An HRP-conjugated goat anti-rabbit IgG

Predicted band size: 68 kDa

true

Western blot - Anti-Calnexin antibody - ER Membrane Marker (AB75801)
  • WB

Supplier Data

Western blot - Anti-Calnexin antibody - ER Membrane Marker (AB75801)

All lanes:

Western blot - Anti-Calnexin antibody - ER Membrane Marker (ab75801) at 1/1000 dilution

All lanes:

Rat Tissue lysates at 15 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG: HRP

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Dog CANX conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P24643

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

References regarding specificity

Horner SM et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353

Myhill N et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615

Yoshimura SI et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.
Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse (By similarity).
See full target information CANX
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Publications (87)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:25917 PubMed40676148

2025

Extracellular vesicles from different regions of the female reproductive tract promote spermatozoa motility and support capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Camilla Fasoli,Elisa Giacomini,Valentina Pavone,Lisett Solano Narduche,Viviana Bonzi,Laura Privitera,Paolo Giardina,Ludovica Bartiromo,Matteo Schimberni,Luca Pagliardini,Enrico Papaleo,Massimo Candiani,Andrea Salonia,Riccardo Vago

Journal of extracellular vesicles 14:e70114 PubMed40673783

2025

ADAM Sheddase Activity Promotes the Detachment of Small Extracellular Vesicles From the Plasma Membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Chloé Bizingre,Zaira Arellano-Anaya,Flavien Picard,Mathéa Pietri,Anne Baudry,Florence Roussel,Clara Bianchi,Aurélie Alleaume-Butaux,Hector Ardila-Osorio,Maryse Romao,Grégory Lavieu,Graça Raposo,Benoit Schneider

Materials today. Bio 32:101878 PubMed40520558

2025

A pH-responsive PEG coating strategy for enhancing the enrichment of small extracellular vesicles towards disease regions with acidic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Zhao,Xinyu Niu,Lei Luo,Ji Yuan,Juntao Zhang,Xin Niu,Hengli Tian,Yunlong Yang,Zhifeng Deng,Yang Wang

Journal of extracellular vesicles 14:e70067 PubMed40241173

2025

Use of the Malaria Protein VAR2CSA for the Detection of Small Extracellular Vesicles to Diagnose Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Zhao,Chenlei Wen,Qi Wang,Yue Qing,Serena Tondi,Chiara Reina,Berina Šabanović,Cherry Yin-Yi Chang,Chu-Hu Lai,Huimin Wang,Mette Ø Agerbaek,Thomas M Clausen,Tobias Gustavsson,Thor G Theander,Ali Salanti,Clara Csilla Meny,Baiyong Shen,Alexandra Aicher,Jiajia Tang,Christopher Heeschen

Food & function 16:3211-3226 PubMed40190095

2025

Cocoa-carob blend acute intake modifies miRNAs related to insulin sensitivity in type 2 diabetic subjects: a randomised controlled nutritional trial.

Applications

Unspecified application

Species

Unspecified reactive species

Marisol Villalva,Esther García-Díez,María Del Carmen López de Las Hazas,Oreste Lo Iacono,José Ignacio Vicente-Díez,Sara García-Cabrera,Marta Alonso-Bernáldez,Alberto Dávalos,María Ángeles Martín,Sonia Ramos,Jara Pérez-Jiménez

ACS nano 19:10078-10092 PubMed40059332

2025

Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Yu,Jianmei Gu,Jiahui Zhang,Maoye Wang,Runbi Ji,Chunlai Feng,Hélder A Santos,Hongbo Zhang,Xu Zhang

The Journal of neuroscience : the official journal of the Society for Neuroscience 45: PubMed39653498

2024

Mitochondrial A Adenosine Receptor as a Mechanism for the Protective Effects of AAR Agonists on Chemotherapy-Induced Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy M Doyle,Kali Janes,Wen Hua Xiao,Grant R Kolar,Hans F Luecke,Michael Anne Gratton,Dilip K Tosh,Kenneth A Jacobson,Gary J Bennett,Daniela Salvemini

The Journal of biological chemistry 300:107833 PubMed39343005

2024

Role of protein domains in trafficking and localization of the voltage-gated sodium channel β2 subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Cortada,Ramon Brugada,Marcel Verges

Regenerative biomaterials 11:rbae112 PubMed39323741

2024

Bone-targeting engineered milk-derived extracellular vesicles for MRI-assisted therapy of osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Huang,Yang Jiang,Yang Cao,Yunchuan Ding,Jinghui Cai,Tingqian Yang,Xin Zhou,Qiang Wu,Danyang Li,Qingyu Liu,Fangping Li

Gene therapy 31:445-454 PubMed39069561

2024

Preclinical evaluation of tissue-selective gene therapies for congenital generalised lipodystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Mansi Tiwari,Ahlima Roumane,Nadine Sommer,Weiping Han,Mirela Delibegović,Justin J Rochford,George D Mcilroy
View all publications
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