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Rabbit Monoclonal Calnexin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Dog, Cat, Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human CANX.

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Images

Western blot - Anti-Calnexin antibody [HL1598] (AB308174), expandable thumbnail
  • Western blot - Anti-Calnexin antibody [HL1598] (AB308174), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (AB308174), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (AB308174), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (AB308174), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human CANX. The exact immunogen used to generate this antibody is proprietary information. Database link P27824

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Expected
Mouse
Tested
Tested
Rat
Tested
Tested
Cat
Tested
Tested
Dog
Tested
Tested

Tested
Tested

Species
Dog
Dilution info
1/500 - 1/3000
Notes

-

Species
Cat
Dilution info
1/500 - 1/3000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Cat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Dog
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Alternative names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

Recommended products

Rabbit Monoclonal Calnexin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Dog, Cat, Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human CANX.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human CANX. The exact immunogen used to generate this antibody is proprietary information. Database link P27824
Carrier free
Yes
Clone number
HL1598
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Associated diseases and disorders

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Western blot - Anti-Calnexin antibody [HL1598] (ab308174)

    Samples were seperated by 7.5% SDS-PAGE.

    All lanes: Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/2000 dilution

    Lane 1: Non-transfected (–) 293T whole cell extracts

    Lane 2: Transfected (+) 293T whole cell extracts

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 67 kDa

  • Western blot - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Western blot - Anti-Calnexin antibody [HL1598] (ab308174)

    Samples were seperated by 7.5% SDS-PAGE.

    All lanes: Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/5000 dilution

    Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg

    Lane 2: Transfected (+) 293T whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 67 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (ab308174)

    Immunohistochemical analysis of paraffin-embedded cat mammary gland tissue labelling Calnexin with ab308174 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (ab308174)

    Immunohistochemical analysis of paraffin-embedded dog pancreas tissue labelling Calnexin with ab308174 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (ab308174)

    Immunohistochemical analysis of paraffin-embedded rat duodenum labeling Calnexin protein with ab308174 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

  • Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (ab308174)

    Immunohistochemical analysis of Paraffin-embedded mouse intestine labeling Calnexin protein with ab308174 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

  • Western blot - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Western blot - Anti-Calnexin antibody [HL1598] (ab308174)

    Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (ab308174) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/1000 dilution

    Lane 1: MDCK cell lysate at 30 µg

    Lane 2: PG-4 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 67.7 kDa

  • Western blot - Anti-Calnexin antibody [HL1598] (ab308174), expandable thumbnail

    Western blot - Anti-Calnexin antibody [HL1598] (ab308174)

    Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (ab308174) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 50 µg

    Lane 2: Rat brain tissue lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 67.7 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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