AB308174

Anti-Calnexin antibody [HL1598]

Recombinant
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Rabbit Recombinant Monoclonal Calnexin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Dog, Cat, Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human CANX.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

8 Images

Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (AB308174)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (AB308174)

Immunohistochemical analysis of Paraffin-embedded mouse intestine labeling Calnexin protein with ab308174 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (AB308174)

Immunohistochemical analysis of paraffin-embedded rat duodenum labeling Calnexin protein with ab308174 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Supplier Data

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/2000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

Transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67 kDa

false

Supplier Data

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67 kDa

false

Supplier Data

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (ab308174) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 50 µg

Lane 2:

Rat brain tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67.7 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (AB308174)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (AB308174)

Immunohistochemical analysis of paraffin-embedded dog pancreas tissue labelling Calnexin with ab308174 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (AB308174)

Immunohistochemical analysis of paraffin-embedded cat mammary gland tissue labelling Calnexin with ab308174 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (ab308174) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Calnexin antibody [HL1598] (ab308174) at 1/1000 dilution

Lane 1:

MDCK cell lysate at 30 µg

Lane 2:

PG-4 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67.7 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1598

Isotype

IgG

Carrier free

Yes

Reacts with

Dog, Cat, Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CANX. The exact immunogen used to generate this antibody is proprietary information.

P27824

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calnexin antibody [HL1598]

Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (AB308174)

Immunohistochemistry paraffin embedded sections - Anti-Calnexin antibody [HL1598] (AB308174)

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (AB308174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [HL1598] (AB308174)

Western blot - Anti-Calnexin antibody [HL1598] (AB308174)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise