AB13504

Anti-Calnexin - ER membrane marker antibody - ER Marker

Name: Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504) | Abcam
Brand: Abcam Limited
SKU: AB13504
Price: 400 USD
Availability: InStock
Rating: 4 (8 reviews)

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What is this?

(8 Reviews)

(39 Publications)

Rabbit Polyclonal Calnexin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Dog CANX aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed 2% Formaldehyde for 20 min at RT. Secondary Antibody : R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody : FITC Goat Anti-Rabbit (green) at 1/200 for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1 : 40000 for 2 hours at RT. Heat Shocked at 42°C for 1h.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/100 for 12 hours at 4°C in HaCaT cells. Fixed by Cold 100% methanol at -20C for 10 minutes. Secondary Antibody : FITC Goat Anti-Rabbit at 1/50 for 1-2 hours at RT in dark. /

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Immunohistochemistry analysis using ab13504 at 1/100 for 1 hour at RT in mouse backskin. Fixed by Bouin's Fixative Solution. Secondary Antibody : FITC Goat Anti-Rabbit (green) at 1/50 for 1 hour at RT.

Unknown

Western blot - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

All lanes:

Western blot - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504) at 1/2000 dilution

All lanes:

Mixture of rat tissue lysates

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Dog CANX aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P24643

Specificity

Weak reactivity with Chicken, Fruitfly (Drosophila melanogaster) and Xenopus laevis.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

The Kaohsiung journal of medical sciences 40:660-670 PubMed38801488

2024

The value of urinary exosomal microRNA-21 in the early diagnosis and prognosis of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Kan Yang,Chao Tian,Lin-Xiong Zhou,Tian-Yu Guan,Gui-Liu Chen,Yi-Ying Zheng,Zheng-Guo Cao

Neurobiology of aging 132:154-174 PubMed37837732

2023

Protein retention in the endoplasmic reticulum rescues Aβ toxicity in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

James H Catterson,Lucy Minkley,Salomé Aspe,Sebastian Judd-Mole,Sofia Moura,Miranda C Dyson,Arjunan Rajasingam,Nathaniel S Woodling,Magda L Atilano,Mumtaz Ahmad,Claire S Durrant,Tara L Spires-Jones,Linda Partridge

Reviews in cardiovascular medicine 23:206 PubMed39077165

2022

MicroRNA Expression Profiles of Epicardial Adipose Tissue-Derived Exosomes in Patients with Coronary Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxing Liu,Ang Gao,Yan Liu,Yan Sun,Dai Zhang,Xuze Lin,Chengping Hu,Yong Zhu,Yu Du,Hongya Han,Yang Li,Shijun Xu,Taoshuai Liu,Chenhan Zhang,Junming Zhu,Ran Dong,Yujie Zhou,Yingxin Zhao

Cell reports 38:110487 PubMed35294880

2022

Cell-autonomous control of intracellular temperature by unsaturation of phospholipid acyl chains.

Applications

Unspecified application

Species

Unspecified reactive species

Akira Murakami,Kohjiro Nagao,Reiko Sakaguchi,Keisuke Kida,Yuji Hara,Yasuo Mori,Kohki Okabe,Yoshie Harada,Masato Umeda

Journal of virology 95:e0078121 PubMed34132567

2021

Fatty Acid Synthase Is Involved in Classical Swine Fever Virus Replication by Interaction with NS4B.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Yun Liu,Xiao-Dong Liang,Chun-Chun Liu,Yan Cheng,Huan Chen,Abdul Sattar Baloch,Jin Zhang,Yun Young Go,Bin Zhou

Cellular and molecular neurobiology 42:2305-2319 PubMed34037901

2021

Comprehensive Analysis of Proteasomal Complexes in Mouse Brain Regions Detects ENO2 as a Potential Partner of the Proteasome in the Striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Niki Esfahanian,Morgan Nelson,Rebecca Autenried,J Scott Pattison,Eduardo Callegari,Khosrow Rezvani

Stem cell research & therapy 11:511 PubMed33246507

2020

Comprehensive proteomic analysis of exosomes derived from human bone marrow, adipose tissue, and umbilical cord mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Gang Wang,Zhi-Yi He,Shuang Liang,Qing Yang,Peng Cheng,An-Min Chen

Oncology reports 45:151-158 PubMed33169801

2020

S100A8 promotes chemoresistance via augmenting autophagy in B‑cell lymphoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhang,Shixia Zhou,Tiejun Zhou,Kaifeng Yuan,Xiaoming Li,Junling Tang

OncoTargets and therapy 13:9701-9719 PubMed33061450

2020

G-MDSCs-Derived Exosomal miRNA-143-3p Promotes Proliferation via Targeting of ITM2B in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Hua Zhou,Zhi-Xian Yao,Zhong Zheng,Jun Yang,Rui Wang,Shi-Jie Fu,Xu-Feng Pan,Zhi-Hong Liu,Ke Wu

Nature communications 11:4837 PubMed32973183

2020

MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzhong Li,Nikola A Ivica,Ting Dong,Dimitrios P Papageorgiou,Yanpu He,Douglas R Brown,Marianna Kleyman,Guangan Hu,Walter W Chen,Lucas B Sullivan,Amanda Del Rosario,Paula T Hammond,Matthew G Vander Heiden,Jianzhu Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calnexin - ER membrane marker antibody - ER Marker

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Western blot - Anti-Calnexin - ER membrane marker antibody - ER Marker (AB13504)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

Product promise