AB39170

Anti-Calpain 1 antibody

Name: Anti-Calpain 1 antibody (ab39170) | Abcam
Brand: Abcam Limited
SKU: AB39170
Price: 565 USD
Availability: InStock

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(38 Publications)

Rabbit Polyclonal Calpain 1 antibody. Suitable for WB, ICC/IF, IHC (PFA fixed) and reacts with Human, Mouse, Rat samples. Cited in 38 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN1.

View Alternative Names

CANPL1, PIG30, CAPN1, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB39170)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB39170)

ICC/IF image of ab39170 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC (PFA fixed), ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CAPN1. The exact immunogen used to generate this antibody is proprietary information.

P07384

Specificity

This antibody binds to reduced and non reduced Calpain 1. It reacts with Domain IV of Calpain 1, and detects both latent and aminoprocessed (activated) Calpain 1. It but does not cross react with the other calpain family members (Calpain 2, calpain 94, ncl 2, ncl 3, etc.).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.

Biological function summary

Calpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.

Pathways

Calpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.

Calpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed : 19617626, PubMed : 21531719, PubMed : 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed : 23707407). Cleaves and activates caspase-7 (CASP7) (PubMed : 19617626).

See full target information CAPN1

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 10:e10737 PubMed40060768

2025

Ultrasound-mediated mechanical forces activate selective tumor cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ajay Tijore,Felix Margadant,Nehal Dwivedi,Leslie Morgan,Mingxi Yao,Anushya Hariharan,Claire Alexandra Zhen Chew,Simon Powell,Glenn Kunnath Bonney,Michael Sheetz

Cell communication and signaling : CCS 22:92 PubMed38303059

2024

T-type voltage-gated channels, Na/Ca-exchanger, and calpain-2 promote photoreceptor cell death in inherited retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yan,Lan Wang,Qian-Lu Yang,Qian-Xi Yang,Xinyi He,Yujie Dong,Zhulin Hu,Mathias W Seeliger,Kangwei Jiao,François Paquet-Durand

Frontiers in cellular neuroscience 17:1199656 PubMed37484825

2023

Prevention of noise-induced hearing loss by calpain inhibitor MDL-28170 is associated with upregulation of PI3K/Akt survival signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruosha Lai,Qiaojun Fang,Fan Wu,Song Pan,Khujista Haque,Su-Hua Sha

Cancer cell international 23:49 PubMed36932402

2023

Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Maren Nicole Stillger,Chia-Yi Chen,Zon Weng Lai,Mujia Li,Agnes Schäfer,Axel Pagenstecher,Christopher Nimsky,Jörg Walter Bartsch,Oliver Schilling

International journal of molecular sciences 23: PubMed36233068

2022

Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model.

Applications

Unspecified application

Species

Unspecified reactive species

Elsayed Metwally,Hatim A Al-Abbadi,Mohamed A Hashem,Yasmina K Mahmoud,Eman A Ahmed,Ahmed I Maaty,Ibrahim E Helal,Mahmoud F Ahmed

Antioxidants (Basel, Switzerland) 11: PubMed35883722

2022

Mitochondrial Oxidative Stress Promotes Cardiac Remodeling in Myocardial Infarction through the Activation of Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco V Souza-Neto,Fabian Islas,Sara Jiménez-González,María Luaces,Bunty Ramchandani,Ana Romero-Miranda,Beatriz Delgado-Valero,Elena Roldan-Molina,Melchor Saiz-Pardo,Mª Ángeles Cerón-Nieto,Luis Ortega-Medina,Ernesto Martínez-Martínez,Victoria Cachofeiro

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5102-5114 PubMed35606145

2022

Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lee-Hsin Wang,Chien-Yu Lin,Yu-Mei Lin,Luc Buée,Nicolas Sergeant,David Blum,Yijuang Chern,Guey-Shin Wang

Bioengineering & translational medicine 6:e10233 PubMed34589605

2021

Enhanced tumor cell killing by ultrasound after microtubule depolymerization.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Singh,Ajay Tijore,Felix Margadant,Chloe Simpson,Deepak Chitkara,Boon Chuan Low,Michael Sheetz

Arteriosclerosis, thrombosis, and vascular biology 41:1694-1709 PubMed33761765

2021

Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Latha Muniappan,Michihiro Okuyama,Aida Javidan,Devi Thiagarajan,Weihua Jiang,Jessica J Moorleghen,Lihua Yang,Anju Balakrishnan,Deborah A Howatt,Haruhito A Uchida,Takaomi C Saido,Venkateswaran Subramanian

Kidney international 100:90-106 PubMed33675847

2021

Calpastatin prevents Angiotensin II-mediated podocyte injury through maintenance of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Imane Bensaada,Blaise Robin,Joëlle Perez,Yann Salemkour,Anna Chipont,Marine Camus,Mathilde Lemoine,Lea Guyonnet,Hélène Lazareth,Emmanuel Letavernier,Carole Hénique,Pierre-Louis Tharaux,Olivia Lenoir

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Calpain 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB39170)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

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