Rabbit Recombinant Monoclonal Calpain 1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected | Expected |
Rat | Tested | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed:19617626, PubMed:21531719, PubMed:2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed:23707407). Cleaves and activates caspase-7 (CASP7) (PubMed:19617626).
CANPL1, PIG30, CAPN1, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP
Rabbit Recombinant Monoclonal Calpain 1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR3319
Affinity purification Protein A
This antibody recognises the large subunit of Calpain
Blue Ice
+4°C
+4°C
Do Not Freeze
ab239930 is the carrier-free version of Anti-Calpain 1 antibody [EPR3319] ab108400.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Calpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.
Calpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.
Calpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.
Calpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calpain 1 antibody [EPR3319] ab108400).
Anti-Calpain 1 antibody [EPR3319] ab108400 was shown to recognize Calpain 1 in wild-type HAP1. A faint cross-reactive band was observed in CAPN1 (Calpain 1) knockout cells at 82 kDa when over-exposed. Due to the immunogen's homology with CAPN2 (Calpain 2 Uniprot: P17655), this band could correspond to the Calpain 2 protein. Please note that cross-reactivity with this protein has not been confirmed experimentally.
All lanes: Western blot - Anti-Calpain 1 antibody [EPR3319] - BSA and Azide free (ab239930)
Lane 1: Wild type HAP1 whole cell lysate
Lane 2: CAPN1 (Calpain 1) knockout HAP1 whole cell lysate
Lane 3: A431 whole cell lysate
Predicted band size: 82 kDa
Overlay histogram showing Jurkat cells stained with Anti-Calpain 1 antibody [EPR3319] ab108400 (red line) at 1/380 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calpain 1 antibody [EPR3319] ab108400).
Anti-Calpain 1 antibody [EPR3319] ab108400 staining Calpain 1 in the T-47D cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/70). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calpain 1 antibody [EPR3319] ab108400).
Anti-Calpain 1 antibody [EPR3319] ab108400 staining Calpain 1 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/200). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calpain 1 antibody [EPR3319] ab108400).
Anti-Calpain 1 antibody [EPR3319] ab108400, unpurified, at 1/500 dilution staining Calpain in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calpain 1 antibody [EPR3319] ab108400).
Anti-Calpain 1 antibody [EPR3319] ab108400, unpurified, at 1/250 dilution staining Calpain in HeLa cells by Immunofluorescence.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Calpain 1 antibody [EPR3319] ab108400).
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