Rabbit Polyclonal Calpain 10 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN10.
View Alternative Names
KIAA1845, CAPN10, Calpain-10, Calcium-activated neutral proteinase 10, CANP 10
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 10 antibody (AB28226)
IHC image of ab28226 staining in normal human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28226, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Calpain 10 antibody (AB28226)
ICC/IF image of ab28226 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28226, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Primary Antibodies
AB108400
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20ul selling size|RabMAb|Recombinant|KO Validated
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Reactivity data
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Calpain 10 contributes to the regulation of intracellular signaling pathways and cellular homeostasis. It plays a role in cytoskeletal remodeling extricating proteins from multiprotein complexes and influencing glucose metabolism. Compared to other calpains Calpain 10 differs structurally as it lacks the typical EF-hand calcium-binding domains. This characteristic suggests its unique mechanism in modulating physiological processes within cells.
Pathways
Calpain 10 interacts with stress-related signaling and metabolic pathways. Particularly it functions within the insulin signaling pathway essential for glucose uptake and metabolism. Calpain 10’s activity affects the insulin receptor substrate (IRS) and is related to the phosphatidylinositol 3-kinase (PI3K) pathway which is important for cellular metabolism and survival. These pathways highlight the relationship between Calpain 10 and metabolic control.
Product protocols
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Target data
Publications (11)
Recent publications for all applications. Explore the full list and refine your search
Oncology reports 43:2093-2104 PubMed32236617
2020
Applications
Unspecified application
Species
Unspecified reactive species
Archives of medical research 50:451-460 PubMed31791003
2019
Applications
Unspecified application
Species
Unspecified reactive species
Diabetes, metabolic syndrome and obesity : targets and therapy 12:1811-1820 PubMed31571956
2019
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Unspecified application
Species
Unspecified reactive species
Toxicology and applied pharmacology 380:114700 PubMed31398423
2019
Applications
Unspecified application
Species
Unspecified reactive species
Journal of the American Heart Association 7:e009167 PubMed30571388
2018
Applications
Unspecified application
Species
Unspecified reactive species
American journal of physiology. Lung cellular and 315:L595-L608 PubMed30024306
2018
Applications
Unspecified application
Species
Unspecified reactive species
Cancer letters 357:186-195 PubMed25434795
2014
Applications
IHC-P
Species
Human
Archives of biochemistry and biophysics 517:144-52 PubMed22179018
2011
Applications
Unspecified application
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Unspecified reactive species
The Journal of biological chemistry 284:13542-50 PubMed19269960
2009
Applications
WB
Species
Mouse
American journal of physiology. Renal physiology 296:F478-86 PubMed19144693
2009
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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