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AB174649

Anti-Calpain 15/SOLH antibody

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(1 Publication)

Rabbit Polyclonal Calpain 15/SOLH antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CAPN15 aa 1-100.

View Alternative Names

SOLH, CAPN15, Calpain-15, Small optic lobes homolog

1 Images
Western blot - Anti-Calpain 15/SOLH antibody (AB174649)
  • WB

Supplier Data

Western blot - Anti-Calpain 15/SOLH antibody (AB174649)

All lanes:

Western blot - Anti-Calpain 15/SOLH antibody (ab174649) at 0.2 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 117 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CAPN15 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O75808

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 15 also known as SOLH (small optic lobes homolog) is a member of the calpain family of proteins which are calcium-dependent cysteine proteases. Calpain 15/SOLH has a molecular mass approximately 75 kDa. Researchers have found its expression in various tissues including the brain heart and skeletal muscle. As with other calpain proteins its function hinges on calcium concentration where it undergoes conformational changes to become active and facilitate the cleavage of its substrates.
Biological function summary

Calpain 15 functions as a calcium-regulated enzyme that participates in the modulation of cytoskeletal dynamics. This protein plays roles in cellular processes like migration signal transduction and apoptosis. It does not specifically associate with well-defined complexes but interacts with multiple cellular structures due to its protease activity. By controlling the degradation of specific proteins Calpain 15 regulates the restructuring of the actin cytoskeleton influencing cell shape and movement.

Pathways

Calpain 15 influences both the integrin signaling and apoptosis pathways. In integrin signaling it modifies the cytoskeleton structure for cell movement and stability. It also plays a role in apoptosis by regulating proteins such as Bcl-2 and caspases which are important for cell survival or death decisions. These interactions indicate its part in complex pathway networks that adjust cellular responses to numerous external stimuli.

Researchers have linked Calpain 15 to neurological disorders including Alzheimer's disease by its involvement in neuronal cell death pathways. Its interaction with proteins like caspase-3 can amplify apoptotic signals contributing to neurodegeneration. Additionally aberrant Calpain 15 activity is connected to muscular dystrophy. The proteolytic imbalance caused by altered Calpain 15 levels impacts muscle integrity and function relating it to proteins like dystrophin which is essential for muscle stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CAPN15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 8:747802 PubMed34595225

2021

LncRNA PVT1 Knockdown Ameliorates Myocardial Ischemia Reperfusion Damage via Suppressing Gasdermin D-Mediated Pyroptosis in Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Cuizhi Li,Huafeng Song,Chunlin Chen,Shaoxian Chen,Qiyu Zhang,Dehui Liu,Jinglong Li,Haojian Dong,Yueheng Wu,Youbin Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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