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AB249597

Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free

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Rabbit Recombinant Monoclonal Calpain 15/SOLH antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

SOLH, CAPN15, Calpain-15, Small optic lobes homolog

3 Images
Flow Cytometry (Intracellular) - Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free (AB249597)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free (AB249597)

This data was developed using ab170926, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling Calpain 15/SOLH with ab170926 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free (AB249597)
  • WB

Supplier Data

Western blot - Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free (AB249597)

This data was developed using ab170926, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calpain 15/SOLH antibody [EPR11078] (<a href='/en-us/products/primary-antibodies/calpain-15-solh-antibody-epr11078-ab170926'>ab170926</a>) at 1/1000 dilution

All lanes:

SH SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 117 kDa

false

Western blot - Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free (AB249597)
  • WB

Supplier Data

Western blot - Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free (AB249597)

This data was developed using ab170926, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calpain 15/SOLH antibody [EPR11078] (<a href='/en-us/products/primary-antibodies/calpain-15-solh-antibody-epr11078-ab170926'>ab170926</a>) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 117 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11078

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249597 is the carrier-free version of ab170926.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 15 also known as SOLH (small optic lobes homolog) is a member of the calpain family of proteins which are calcium-dependent cysteine proteases. Calpain 15/SOLH has a molecular mass approximately 75 kDa. Researchers have found its expression in various tissues including the brain heart and skeletal muscle. As with other calpain proteins its function hinges on calcium concentration where it undergoes conformational changes to become active and facilitate the cleavage of its substrates.
Biological function summary

Calpain 15 functions as a calcium-regulated enzyme that participates in the modulation of cytoskeletal dynamics. This protein plays roles in cellular processes like migration signal transduction and apoptosis. It does not specifically associate with well-defined complexes but interacts with multiple cellular structures due to its protease activity. By controlling the degradation of specific proteins Calpain 15 regulates the restructuring of the actin cytoskeleton influencing cell shape and movement.

Pathways

Calpain 15 influences both the integrin signaling and apoptosis pathways. In integrin signaling it modifies the cytoskeleton structure for cell movement and stability. It also plays a role in apoptosis by regulating proteins such as Bcl-2 and caspases which are important for cell survival or death decisions. These interactions indicate its part in complex pathway networks that adjust cellular responses to numerous external stimuli.

Researchers have linked Calpain 15 to neurological disorders including Alzheimer's disease by its involvement in neuronal cell death pathways. Its interaction with proteins like caspase-3 can amplify apoptotic signals contributing to neurodegeneration. Additionally aberrant Calpain 15 activity is connected to muscular dystrophy. The proteolytic imbalance caused by altered Calpain 15 levels impacts muscle integrity and function relating it to proteins like dystrophin which is essential for muscle stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CAPN15
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com