Anti-Calpain 2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 2 antibody (AB236650)

Paraffin-embedded human appendix tissue stained for Calpain 2 using ab236650 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 2 antibody (AB236650)

Paraffin-embedded human tonsil tissue stained for Calpain 2 using ab236650 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 2 antibody (AB236650)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Calpain 2 (green) using ab236650 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

• IP

Supplier Data

Immunoprecipitation - Anti-Calpain 2 antibody (AB236650)

Calpain 2 was immunoprecipitated from 500 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab236650 at 1/200 dilution.

Lane 1 : Rabbit control IgG IP in HeLa whole cell lysate.

Lane 2 : ab236650 IP in HeLa whole cell lysate.

Lane 3 : HeLa whole cell lysate 10 μg (Input).

For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-Calpain 2 antibody (ab236650)

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-Calpain 2 antibody (AB236650)

All lanes:

Western blot - Anti-Calpain 2 antibody (ab236650) at 1/1000 dilution

Lane 1:

Mouse lung lysate

Lane 2:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 4:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 6:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 7:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-Calpain 2 antibody (AB236650)

All lanes:

Western blot - Anti-Calpain 2 antibody (ab236650) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 80 kDa

false